An autonomously propagating luciferase-encoding vector for Dictyostelium discoideum
We have constructed a luc reporter vector for Dictyostelium discoideum using a 626-bp fragment from the nuclear-associated plasmid Ddp2. The ori from Ddp2 is localized within this fragment and was used to provide an autonomous replication sequence for the reporter vector. This reporter vector was st...
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| Veröffentlicht in: | Gene 1994, Vol.150 (2), p.293-298 |
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| container_title | Gene |
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| creator | Yin, Yizhong Williamson, Brian D. Rutherford, Charles L. |
| description | We have constructed a
luc reporter vector for
Dictyostelium discoideum using a 626-bp fragment from the nuclear-associated plasmid Ddp2. The
ori from Ddp2 is localized within this fragment and was used to provide an autonomous replication sequence for the reporter vector. This reporter vector was stably retained in
D. discoideum AX3K cells without alteration. The vector molecule was also found to exist in relatively low copy number compared to other
Dictyostelium vectors in the transformed cells. We demonstrated the utility of this vector as a reporter vector with glycogen synthase promoter/
luc fusions of varying sizes. |
| doi_str_mv | 10.1016/0378-1119(94)90440-5 |
| format | Article |
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luc reporter vector for
Dictyostelium discoideum using a 626-bp fragment from the nuclear-associated plasmid Ddp2. The
ori from Ddp2 is localized within this fragment and was used to provide an autonomous replication sequence for the reporter vector. This reporter vector was stably retained in
D. discoideum AX3K cells without alteration. The vector molecule was also found to exist in relatively low copy number compared to other
Dictyostelium vectors in the transformed cells. We demonstrated the utility of this vector as a reporter vector with glycogen synthase promoter/
luc fusions of varying sizes.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(94)90440-5</identifier><identifier>PMID: 7821794</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Cloning, Molecular - methods ; Dictyostelium ; Dictyostelium discoideum ; Genetic Vectors ; Glycogen Synthase - genetics ; Glycogen synthase promoter ; Luciferases - biosynthesis ; Luciferases - genetics ; Molecular Sequence Data ; nuclear-associated plasmid ; Plasmids ; Promoter Regions, Genetic ; Recombinant Proteins - biosynthesis ; reporter gene ; Restriction Mapping ; Sequence Deletion</subject><ispartof>Gene, 1994, Vol.150 (2), p.293-298</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-f041b46cf06bea2f0318106d3a8f0fd33b5f35b633014e7cc082235f23e3f7063</citedby><cites>FETCH-LOGICAL-c388t-f041b46cf06bea2f0318106d3a8f0fd33b5f35b633014e7cc082235f23e3f7063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(94)90440-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7821794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Yizhong</creatorcontrib><creatorcontrib>Williamson, Brian D.</creatorcontrib><creatorcontrib>Rutherford, Charles L.</creatorcontrib><title>An autonomously propagating luciferase-encoding vector for Dictyostelium discoideum</title><title>Gene</title><addtitle>Gene</addtitle><description>We have constructed a
luc reporter vector for
Dictyostelium discoideum using a 626-bp fragment from the nuclear-associated plasmid Ddp2. The
ori from Ddp2 is localized within this fragment and was used to provide an autonomous replication sequence for the reporter vector. This reporter vector was stably retained in
D. discoideum AX3K cells without alteration. The vector molecule was also found to exist in relatively low copy number compared to other
Dictyostelium vectors in the transformed cells. We demonstrated the utility of this vector as a reporter vector with glycogen synthase promoter/
luc fusions of varying sizes.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular - methods</subject><subject>Dictyostelium</subject><subject>Dictyostelium discoideum</subject><subject>Genetic Vectors</subject><subject>Glycogen Synthase - genetics</subject><subject>Glycogen synthase promoter</subject><subject>Luciferases - biosynthesis</subject><subject>Luciferases - genetics</subject><subject>Molecular Sequence Data</subject><subject>nuclear-associated plasmid</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>reporter gene</subject><subject>Restriction Mapping</subject><subject>Sequence Deletion</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LJDEQhsOi6Oj6D1bok-ih3UonnaQvwuDXLgh70D2HdLoike7OmHQL8-_NOINHLSgKqt76egj5ReGSAhW_gUlVUkqb84ZfNMA5lPUPsqBKNiUAU3tk8Sk5JEcpvUC2uq4OyIFUFZUNX5DH5ViYeQpjGMKc-nWximFlns3kx-ein613GE3CEkcbuk3uDe0UYuGy33g7rUOasPfzUHQ-2eA7nIefZN-ZPuHJLh6T_3e3T9d_yod_93-vlw-lZUpNpQNOWy6sA9GiqRwwqiiIjhnlwHWMtbVjdSsYA8pRWguqqljtKobMSRDsmJxt5-abX2dMkx7yDdj3ZsT8jJai4Vxw9a2QCsmoBJ6FfCu0MaQU0elV9IOJa01Bb6DrDVG9Iaobrj-g6zq3ne7mz-2A3WfTjnKuX23rmGm8eYw6WZ-JYudjxqm74L9e8A5aCpF0</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Yin, Yizhong</creator><creator>Williamson, Brian D.</creator><creator>Rutherford, Charles L.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>An autonomously propagating luciferase-encoding vector for Dictyostelium discoideum</title><author>Yin, Yizhong ; Williamson, Brian D. ; Rutherford, Charles L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-f041b46cf06bea2f0318106d3a8f0fd33b5f35b633014e7cc082235f23e3f7063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular - methods</topic><topic>Dictyostelium</topic><topic>Dictyostelium discoideum</topic><topic>Genetic Vectors</topic><topic>Glycogen Synthase - genetics</topic><topic>Glycogen synthase promoter</topic><topic>Luciferases - biosynthesis</topic><topic>Luciferases - genetics</topic><topic>Molecular Sequence Data</topic><topic>nuclear-associated plasmid</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>reporter gene</topic><topic>Restriction Mapping</topic><topic>Sequence Deletion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Yizhong</creatorcontrib><creatorcontrib>Williamson, Brian D.</creatorcontrib><creatorcontrib>Rutherford, Charles L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Yizhong</au><au>Williamson, Brian D.</au><au>Rutherford, Charles L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An autonomously propagating luciferase-encoding vector for Dictyostelium discoideum</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1994</date><risdate>1994</risdate><volume>150</volume><issue>2</issue><spage>293</spage><epage>298</epage><pages>293-298</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>We have constructed a
luc reporter vector for
Dictyostelium discoideum using a 626-bp fragment from the nuclear-associated plasmid Ddp2. The
ori from Ddp2 is localized within this fragment and was used to provide an autonomous replication sequence for the reporter vector. This reporter vector was stably retained in
D. discoideum AX3K cells without alteration. The vector molecule was also found to exist in relatively low copy number compared to other
Dictyostelium vectors in the transformed cells. We demonstrated the utility of this vector as a reporter vector with glycogen synthase promoter/
luc fusions of varying sizes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7821794</pmid><doi>10.1016/0378-1119(94)90440-5</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1994, Vol.150 (2), p.293-298 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76944648 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Base Sequence Cloning, Molecular - methods Dictyostelium Dictyostelium discoideum Genetic Vectors Glycogen Synthase - genetics Glycogen synthase promoter Luciferases - biosynthesis Luciferases - genetics Molecular Sequence Data nuclear-associated plasmid Plasmids Promoter Regions, Genetic Recombinant Proteins - biosynthesis reporter gene Restriction Mapping Sequence Deletion |
| title | An autonomously propagating luciferase-encoding vector for Dictyostelium discoideum |
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