A flow cytometric method for intracellular labeling and purification of rare neuronal populations: Isolation of fixed neurophysin neurons

A method is described for flow cytometric analysis and fluorescence-activated cell sorting of small populations of neurons following dissociation of fixed brain tissue and immunofluorescent labeling of intracellular antigens. This method has been successfully applied to neurophysin-containing magnoc...

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Veröffentlicht in:	Brain research 1986-06, Vol.376 (2), p.310-319
Hauptverfasser:	Paden, Charles M., Berglund, Deborah L., Hapner, Sharon J., Welsh, Clem J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Separation Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Flow Cytometry fluorescence-activated cell sorting Fundamental and applied biological sciences. Psychology Neurophysins - metabolism neurphysin Paraventricular Hypothalamic Nucleus - cytology Paraventricular Hypothalamic Nucleus - metabolism paraventricular nucleus Rats supraoptic nucleus Supraoptic Nucleus - cytology Supraoptic Nucleus - metabolism Vertebrates: nervous system and sense organs
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