Ontogeny of glucocorticoid and D2 receptors in the rat pituitary: an in situ hybridization study
The expression of glucocorticoid and D2 dopamine receptors (GR and D2R) during rat pituitary ontogenesis was studied by in situ hybridization (ISH). On early stages, E13–E14, a weak specific signal for GR mRNA was obvious in the whole Rathke's pouch (RP) whereas subsequently, from E17–E18, stro...
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| Veröffentlicht in: | Molecular and cellular endocrinology 1994-10, Vol.105 (1), p.65-75 |
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| creator | René, F. Hindelang, C. Stoeckel, M.E. Félix, J.M. |
| description | The expression of glucocorticoid and D2 dopamine receptors (GR and D2R) during rat pituitary ontogenesis was studied by in situ hybridization (ISH). On early stages, E13–E14, a weak specific signal for GR mRNA was obvious in the whole Rathke's pouch (RP) whereas subsequently, from E17–E18, strong labelling was restricted to the anterior lobe (AL) and the neural lobe (NL). At the same time, D2R mRNAs appeared in the intermediate lobe (IL) and the long isoform of the D2R (D2R 444) was detectable with specific probes. On the postnatal stages, until adult, GR mRNA, if present, was always undetectable in the IL using the conventional ISH technique. These data indicate a possible early regulation of POMC gene expression by glucocorticoid in corticotrophic cells of the AL and by dopamine in the melanotrophic cells of the IL. The possibility of a negative regulation of GR mRNA by dopamine (DA) in the IL as soon as E17 is discussed. |
| doi_str_mv | 10.1016/0303-7207(94)90036-1 |
| format | Article |
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On early stages, E13–E14, a weak specific signal for GR mRNA was obvious in the whole Rathke's pouch (RP) whereas subsequently, from E17–E18, strong labelling was restricted to the anterior lobe (AL) and the neural lobe (NL). At the same time, D2R mRNAs appeared in the intermediate lobe (IL) and the long isoform of the D2R (D2R 444) was detectable with specific probes. On the postnatal stages, until adult, GR mRNA, if present, was always undetectable in the IL using the conventional ISH technique. These data indicate a possible early regulation of POMC gene expression by glucocorticoid in corticotrophic cells of the AL and by dopamine in the melanotrophic cells of the IL. The possibility of a negative regulation of GR mRNA by dopamine (DA) in the IL as soon as E17 is discussed.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/0303-7207(94)90036-1</identifier><identifier>PMID: 7821719</identifier><identifier>CODEN: MCEND6</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cell receptors ; Cell structures and functions ; Development ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors ; In Situ Hybridization ; Intermediate lobe ; Molecular and cellular biology ; Pituitary Gland - embryology ; Pituitary Gland - growth & development ; Pituitary Gland - metabolism ; Pregnancy ; Pro-Opiomelanocortin - genetics ; Rats ; Rats, Wistar ; Receptor ; Receptors, Dopamine D2 - genetics ; Receptors, Glucocorticoid - genetics ; RNA, Messenger - metabolism</subject><ispartof>Molecular and cellular endocrinology, 1994-10, Vol.105 (1), p.65-75</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-7e6918682af6af55bbf897e3c133dfc98d72af4295e91629573b2e079db86b063</citedby><cites>FETCH-LOGICAL-c386t-7e6918682af6af55bbf897e3c133dfc98d72af4295e91629573b2e079db86b063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0303720794900361$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4263548$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7821719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>René, F.</creatorcontrib><creatorcontrib>Hindelang, C.</creatorcontrib><creatorcontrib>Stoeckel, M.E.</creatorcontrib><creatorcontrib>Félix, J.M.</creatorcontrib><title>Ontogeny of glucocorticoid and D2 receptors in the rat pituitary: an in situ hybridization study</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>The expression of glucocorticoid and D2 dopamine receptors (GR and D2R) during rat pituitary ontogenesis was studied by in situ hybridization (ISH). On early stages, E13–E14, a weak specific signal for GR mRNA was obvious in the whole Rathke's pouch (RP) whereas subsequently, from E17–E18, strong labelling was restricted to the anterior lobe (AL) and the neural lobe (NL). At the same time, D2R mRNAs appeared in the intermediate lobe (IL) and the long isoform of the D2R (D2R 444) was detectable with specific probes. On the postnatal stages, until adult, GR mRNA, if present, was always undetectable in the IL using the conventional ISH technique. These data indicate a possible early regulation of POMC gene expression by glucocorticoid in corticotrophic cells of the AL and by dopamine in the melanotrophic cells of the IL. The possibility of a negative regulation of GR mRNA by dopamine (DA) in the IL as soon as E17 is discussed.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Development</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</subject><subject>In Situ Hybridization</subject><subject>Intermediate lobe</subject><subject>Molecular and cellular biology</subject><subject>Pituitary Gland - embryology</subject><subject>Pituitary Gland - growth & development</subject><subject>Pituitary Gland - metabolism</subject><subject>Pregnancy</subject><subject>Pro-Opiomelanocortin - genetics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptor</subject><subject>Receptors, Dopamine D2 - genetics</subject><subject>Receptors, Glucocorticoid - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vEzEQhi1EVdKWfwCSDwiVw4I_dv3BAQm1QCtV6qU9u157NjHarIPtRUp_fR0S5chppHmfGc08CL2j5DMlVHwhnPBGMiIvdftJE8JFQ1-hBVWSNYp08jVaHJE36Czn34QQ2TF1ik6lYlRSvUBP91OJS5i2OA54Oc4uuphKcDF4bCePrxlO4GBTYso4TLisACdb8CaUORSbtl8rtgtybeDVtk_Bh2dbQqytMvvtBToZ7Jjh7aGeo8efPx6ubpq7-1-3V9_vGseVKI0EoakSitlB2KHr-n5QWgJ3lHM_OK28rFHLdAeailok7xkQqX2vRE8EP0cf93s3Kf6ZIRezDtnBONoJ4pyNFLolvNMVbPegSzHnBIPZpLCunxhKzE6s2VkzO2tGt-afWEPr2PvD_rlfgz8OHUzW_MMht9nZcUh2ciEfsZYJ3rWqYt_2GFQXfwMkk12AyYEP1XMxPob_3_ECqUSUVg</recordid><startdate>19941001</startdate><enddate>19941001</enddate><creator>René, F.</creator><creator>Hindelang, C.</creator><creator>Stoeckel, M.E.</creator><creator>Félix, J.M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941001</creationdate><title>Ontogeny of glucocorticoid and D2 receptors in the rat pituitary: an in situ hybridization study</title><author>René, F. ; Hindelang, C. ; Stoeckel, M.E. ; Félix, J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-7e6918682af6af55bbf897e3c133dfc98d72af4295e91629573b2e079db86b063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Development</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</topic><topic>In Situ Hybridization</topic><topic>Intermediate lobe</topic><topic>Molecular and cellular biology</topic><topic>Pituitary Gland - embryology</topic><topic>Pituitary Gland - growth & development</topic><topic>Pituitary Gland - metabolism</topic><topic>Pregnancy</topic><topic>Pro-Opiomelanocortin - genetics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptor</topic><topic>Receptors, Dopamine D2 - genetics</topic><topic>Receptors, Glucocorticoid - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>René, F.</creatorcontrib><creatorcontrib>Hindelang, C.</creatorcontrib><creatorcontrib>Stoeckel, M.E.</creatorcontrib><creatorcontrib>Félix, J.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>René, F.</au><au>Hindelang, C.</au><au>Stoeckel, M.E.</au><au>Félix, J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ontogeny of glucocorticoid and D2 receptors in the rat pituitary: an in situ hybridization study</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>105</volume><issue>1</issue><spage>65</spage><epage>75</epage><pages>65-75</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><coden>MCEND6</coden><abstract>The expression of glucocorticoid and D2 dopamine receptors (GR and D2R) during rat pituitary ontogenesis was studied by in situ hybridization (ISH). On early stages, E13–E14, a weak specific signal for GR mRNA was obvious in the whole Rathke's pouch (RP) whereas subsequently, from E17–E18, strong labelling was restricted to the anterior lobe (AL) and the neural lobe (NL). At the same time, D2R mRNAs appeared in the intermediate lobe (IL) and the long isoform of the D2R (D2R 444) was detectable with specific probes. On the postnatal stages, until adult, GR mRNA, if present, was always undetectable in the IL using the conventional ISH technique. These data indicate a possible early regulation of POMC gene expression by glucocorticoid in corticotrophic cells of the AL and by dopamine in the melanotrophic cells of the IL. The possibility of a negative regulation of GR mRNA by dopamine (DA) in the IL as soon as E17 is discussed.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>7821719</pmid><doi>10.1016/0303-7207(94)90036-1</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cell receptors Cell structures and functions Development Female Fundamental and applied biological sciences. Psychology Gene Expression Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors In Situ Hybridization Intermediate lobe Molecular and cellular biology Pituitary Gland - embryology Pituitary Gland - growth & development Pituitary Gland - metabolism Pregnancy Pro-Opiomelanocortin - genetics Rats Rats, Wistar Receptor Receptors, Dopamine D2 - genetics Receptors, Glucocorticoid - genetics RNA, Messenger - metabolism |
| title | Ontogeny of glucocorticoid and D2 receptors in the rat pituitary: an in situ hybridization study |
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