Prostaglandin production during spontaneous labor and after treatment with RU486 in pregnant rhesus macaques
The production of prostaglandins from amnion, chorion, decidua, and myometrium was studied in a superfusion system to determine the level of prostaglandin production during late pregnancy, during spontaneous labor, and after in vivo treatment with RU486. Tissues were divided into three groups: those...
Gespeichert in:
| Veröffentlicht in: | Biology of reproduction 1994-10, Vol.51 (4), p.760-765 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 765 |
|---|---|
| container_issue | 4 |
| container_start_page | 760 |
| container_title | Biology of reproduction |
| container_volume | 51 |
| creator | Haluska, G J Kaler, C A Cook, M J Novy, M J |
| description | The production of prostaglandins from amnion, chorion, decidua, and myometrium was studied in a superfusion system to determine
the level of prostaglandin production during late pregnancy, during spontaneous labor, and after in vivo treatment with RU486.
Tissues were divided into three groups: those from pregnant control animals, those from animals receiving RU486 (20 mg/kg/day
for 3 days) in vivo, and those from animals in spontaneous term labor. Tissues were collected at cesarean section and placed
in the superfusion system. After a 30-min equilibration period, fractions of media were collected after passing through the
tissue chambers and were assayed for prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E2 (PGE2). The results showed a
significant increase in PGF2 alpha from decidua of animals treated with RU486 compared to pregnant controls (pregnant: 3.01
+/- 0.45 ng/g/min; RU486: 4.45 +/- 0.28 ng/g/min; p < 0.05). There was a dramatic increase in PGE2 production by amnion from
animals in spontaneous labor but not after RU486 treatment (pregnant: 0.21 +/- 0.06 ng/g/min; RU486: 0.56 +/- 0.09 ng/g/min;
spontaneous labor: 5.83 +/- 0.43 ng/g/min; p < 0.01). We conclude that while progesterone is important for maintaining uterine
quiescence during pregnancy, progesterone receptor blockade by RU486 does not lead to an increase in PGE2 production by amnion
as is shown during normal spontaneous labor. |
| doi_str_mv | 10.1095/biolreprod51.4.760 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_76939563</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76939563</sourcerecordid><originalsourceid>FETCH-LOGICAL-h236t-feddc5202d80b27553525f741d6799c2d811c6393b7a474729b0880871c27cb33</originalsourceid><addsrcrecordid>eNotkEtLxDAUhYMo4zj6BwQhG911zKNJmqWILxhQxFmXtE3bSJqOSUrx35txZnXhnO-ey7kAXGO0xkiy-8qM1uudHxuG1_lacHQClpgRmQnCi1OwRAjxjFJOz8FFCN8I4ZwSugALUWCZs2IJ7IcfQ1SdVa4xDu6zpjqa0cFm8sZ1MOxGF5XT4xSgVdXoYSKhaqP2MHqt4qBdhLOJPfzc5gWH_ym6cyrJvtch7Q2qVj-TDpfgrFU26KvjXIHt89PX42u2eX95e3zYZD2hPGatbpqaEUSaAlVEMEYZYa3IccOFlHWSMa45lbQSKhe5ILJCRYEKgWsi6orSFbg75KY6-7uxHEyotbWHHqXgkkrG9-DNEZyqQTflzptB-d_y-J7k3x783nT9bLwuw6CsTTQt53lmuMxTGKJ_bnJ3UQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76939563</pqid></control><display><type>article</type><title>Prostaglandin production during spontaneous labor and after treatment with RU486 in pregnant rhesus macaques</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Haluska, G J ; Kaler, C A ; Cook, M J ; Novy, M J</creator><creatorcontrib>Haluska, G J ; Kaler, C A ; Cook, M J ; Novy, M J</creatorcontrib><description>The production of prostaglandins from amnion, chorion, decidua, and myometrium was studied in a superfusion system to determine
the level of prostaglandin production during late pregnancy, during spontaneous labor, and after in vivo treatment with RU486.
Tissues were divided into three groups: those from pregnant control animals, those from animals receiving RU486 (20 mg/kg/day
for 3 days) in vivo, and those from animals in spontaneous term labor. Tissues were collected at cesarean section and placed
in the superfusion system. After a 30-min equilibration period, fractions of media were collected after passing through the
tissue chambers and were assayed for prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E2 (PGE2). The results showed a
significant increase in PGF2 alpha from decidua of animals treated with RU486 compared to pregnant controls (pregnant: 3.01
+/- 0.45 ng/g/min; RU486: 4.45 +/- 0.28 ng/g/min; p < 0.05). There was a dramatic increase in PGE2 production by amnion from
animals in spontaneous labor but not after RU486 treatment (pregnant: 0.21 +/- 0.06 ng/g/min; RU486: 0.56 +/- 0.09 ng/g/min;
spontaneous labor: 5.83 +/- 0.43 ng/g/min; p < 0.01). We conclude that while progesterone is important for maintaining uterine
quiescence during pregnancy, progesterone receptor blockade by RU486 does not lead to an increase in PGE2 production by amnion
as is shown during normal spontaneous labor.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod51.4.760</identifier><identifier>PMID: 7819458</identifier><language>eng</language><publisher>United States: Society for the Study of Reproduction</publisher><subject>Amnion - drug effects ; Amnion - metabolism ; Animals ; Chorion - drug effects ; Chorion - metabolism ; Decidua - drug effects ; Decidua - metabolism ; Dinoprost - biosynthesis ; Dinoprostone - biosynthesis ; Female ; Kinetics ; Labor, Obstetric - physiology ; Macaca mulatta ; Mifepristone - pharmacology ; Myometrium - drug effects ; Myometrium - metabolism ; Pregnancy ; Prostaglandins - biosynthesis</subject><ispartof>Biology of reproduction, 1994-10, Vol.51 (4), p.760-765</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7819458$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haluska, G J</creatorcontrib><creatorcontrib>Kaler, C A</creatorcontrib><creatorcontrib>Cook, M J</creatorcontrib><creatorcontrib>Novy, M J</creatorcontrib><title>Prostaglandin production during spontaneous labor and after treatment with RU486 in pregnant rhesus macaques</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The production of prostaglandins from amnion, chorion, decidua, and myometrium was studied in a superfusion system to determine
the level of prostaglandin production during late pregnancy, during spontaneous labor, and after in vivo treatment with RU486.
Tissues were divided into three groups: those from pregnant control animals, those from animals receiving RU486 (20 mg/kg/day
for 3 days) in vivo, and those from animals in spontaneous term labor. Tissues were collected at cesarean section and placed
in the superfusion system. After a 30-min equilibration period, fractions of media were collected after passing through the
tissue chambers and were assayed for prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E2 (PGE2). The results showed a
significant increase in PGF2 alpha from decidua of animals treated with RU486 compared to pregnant controls (pregnant: 3.01
+/- 0.45 ng/g/min; RU486: 4.45 +/- 0.28 ng/g/min; p < 0.05). There was a dramatic increase in PGE2 production by amnion from
animals in spontaneous labor but not after RU486 treatment (pregnant: 0.21 +/- 0.06 ng/g/min; RU486: 0.56 +/- 0.09 ng/g/min;
spontaneous labor: 5.83 +/- 0.43 ng/g/min; p < 0.01). We conclude that while progesterone is important for maintaining uterine
quiescence during pregnancy, progesterone receptor blockade by RU486 does not lead to an increase in PGE2 production by amnion
as is shown during normal spontaneous labor.</description><subject>Amnion - drug effects</subject><subject>Amnion - metabolism</subject><subject>Animals</subject><subject>Chorion - drug effects</subject><subject>Chorion - metabolism</subject><subject>Decidua - drug effects</subject><subject>Decidua - metabolism</subject><subject>Dinoprost - biosynthesis</subject><subject>Dinoprostone - biosynthesis</subject><subject>Female</subject><subject>Kinetics</subject><subject>Labor, Obstetric - physiology</subject><subject>Macaca mulatta</subject><subject>Mifepristone - pharmacology</subject><subject>Myometrium - drug effects</subject><subject>Myometrium - metabolism</subject><subject>Pregnancy</subject><subject>Prostaglandins - biosynthesis</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkEtLxDAUhYMo4zj6BwQhG911zKNJmqWILxhQxFmXtE3bSJqOSUrx35txZnXhnO-ey7kAXGO0xkiy-8qM1uudHxuG1_lacHQClpgRmQnCi1OwRAjxjFJOz8FFCN8I4ZwSugALUWCZs2IJ7IcfQ1SdVa4xDu6zpjqa0cFm8sZ1MOxGF5XT4xSgVdXoYSKhaqP2MHqt4qBdhLOJPfzc5gWH_ym6cyrJvtch7Q2qVj-TDpfgrFU26KvjXIHt89PX42u2eX95e3zYZD2hPGatbpqaEUSaAlVEMEYZYa3IccOFlHWSMa45lbQSKhe5ILJCRYEKgWsi6orSFbg75KY6-7uxHEyotbWHHqXgkkrG9-DNEZyqQTflzptB-d_y-J7k3x783nT9bLwuw6CsTTQt53lmuMxTGKJ_bnJ3UQ</recordid><startdate>19941001</startdate><enddate>19941001</enddate><creator>Haluska, G J</creator><creator>Kaler, C A</creator><creator>Cook, M J</creator><creator>Novy, M J</creator><general>Society for the Study of Reproduction</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19941001</creationdate><title>Prostaglandin production during spontaneous labor and after treatment with RU486 in pregnant rhesus macaques</title><author>Haluska, G J ; Kaler, C A ; Cook, M J ; Novy, M J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h236t-feddc5202d80b27553525f741d6799c2d811c6393b7a474729b0880871c27cb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amnion - drug effects</topic><topic>Amnion - metabolism</topic><topic>Animals</topic><topic>Chorion - drug effects</topic><topic>Chorion - metabolism</topic><topic>Decidua - drug effects</topic><topic>Decidua - metabolism</topic><topic>Dinoprost - biosynthesis</topic><topic>Dinoprostone - biosynthesis</topic><topic>Female</topic><topic>Kinetics</topic><topic>Labor, Obstetric - physiology</topic><topic>Macaca mulatta</topic><topic>Mifepristone - pharmacology</topic><topic>Myometrium - drug effects</topic><topic>Myometrium - metabolism</topic><topic>Pregnancy</topic><topic>Prostaglandins - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haluska, G J</creatorcontrib><creatorcontrib>Kaler, C A</creatorcontrib><creatorcontrib>Cook, M J</creatorcontrib><creatorcontrib>Novy, M J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haluska, G J</au><au>Kaler, C A</au><au>Cook, M J</au><au>Novy, M J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prostaglandin production during spontaneous labor and after treatment with RU486 in pregnant rhesus macaques</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>51</volume><issue>4</issue><spage>760</spage><epage>765</epage><pages>760-765</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>The production of prostaglandins from amnion, chorion, decidua, and myometrium was studied in a superfusion system to determine
the level of prostaglandin production during late pregnancy, during spontaneous labor, and after in vivo treatment with RU486.
Tissues were divided into three groups: those from pregnant control animals, those from animals receiving RU486 (20 mg/kg/day
for 3 days) in vivo, and those from animals in spontaneous term labor. Tissues were collected at cesarean section and placed
in the superfusion system. After a 30-min equilibration period, fractions of media were collected after passing through the
tissue chambers and were assayed for prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E2 (PGE2). The results showed a
significant increase in PGF2 alpha from decidua of animals treated with RU486 compared to pregnant controls (pregnant: 3.01
+/- 0.45 ng/g/min; RU486: 4.45 +/- 0.28 ng/g/min; p < 0.05). There was a dramatic increase in PGE2 production by amnion from
animals in spontaneous labor but not after RU486 treatment (pregnant: 0.21 +/- 0.06 ng/g/min; RU486: 0.56 +/- 0.09 ng/g/min;
spontaneous labor: 5.83 +/- 0.43 ng/g/min; p < 0.01). We conclude that while progesterone is important for maintaining uterine
quiescence during pregnancy, progesterone receptor blockade by RU486 does not lead to an increase in PGE2 production by amnion
as is shown during normal spontaneous labor.</abstract><cop>United States</cop><pub>Society for the Study of Reproduction</pub><pmid>7819458</pmid><doi>10.1095/biolreprod51.4.760</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 1994-10, Vol.51 (4), p.760-765 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76939563 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Amnion - drug effects Amnion - metabolism Animals Chorion - drug effects Chorion - metabolism Decidua - drug effects Decidua - metabolism Dinoprost - biosynthesis Dinoprostone - biosynthesis Female Kinetics Labor, Obstetric - physiology Macaca mulatta Mifepristone - pharmacology Myometrium - drug effects Myometrium - metabolism Pregnancy Prostaglandins - biosynthesis |
| title | Prostaglandin production during spontaneous labor and after treatment with RU486 in pregnant rhesus macaques |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T19%3A48%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prostaglandin%20production%20during%20spontaneous%20labor%20and%20after%20treatment%20with%20RU486%20in%20pregnant%20rhesus%20macaques&rft.jtitle=Biology%20of%20reproduction&rft.au=Haluska,%20G%20J&rft.date=1994-10-01&rft.volume=51&rft.issue=4&rft.spage=760&rft.epage=765&rft.pages=760-765&rft.issn=0006-3363&rft.eissn=1529-7268&rft_id=info:doi/10.1095/biolreprod51.4.760&rft_dat=%3Cproquest_pubme%3E76939563%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76939563&rft_id=info:pmid/7819458&rfr_iscdi=true |