Activation of the vasopressin-sensitive water permeability pathway in the toad bladder by N-ethyl maleimide
Vasopressin stimulates transepithelial water flow in the toad urinary bladder. We report here that N-ethyl maleimide (NEM) (0.1 mM) produces a similar increase in osmotic water flow when applied to the mucosal surface of the tissue. NEM-induced water flow is sensitive to inhibitors of hormone-induce...
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| Veröffentlicht in: | Experimental physiology 1994-09, Vol.79 (5), p.775-795 |
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| creator | Marples, D Bourguet, J Taylor, A |
| description | Vasopressin stimulates transepithelial water flow in the toad urinary bladder. We report here that N-ethyl maleimide (NEM)
(0.1 mM) produces a similar increase in osmotic water flow when applied to the mucosal surface of the tissue. NEM-induced
water flow is sensitive to inhibitors of hormone-induced water flow, including serosal acidification, or exposure to quinidine
or cytoskeleton-disruptive drugs. NEM-induced water flow is additive with that induced by a submaximal, but not a maximal,
dose of vasopressin. The response to mucosal NEM is not reversed on removal of the reagent, but established NEM-induced water
flow can be inhibited by serosal acidification or quinidine. Like vasopressin, mucosal NEM induces the appearance of fusion
profiles and intramembranous particle aggregates (putative water channels) in the apical plasma membrane of the granular cells,
and the incidence of particle aggregates correlates with water flow. NEM does not cause an increase in intracellular cAMP.
Our data suggest that NEM stimulates transepithelial water flow by irreversibly activating cellular mechanisms normally triggered
by vasopressin, hence causing the insertion of water channels. |
| doi_str_mv | 10.1113/expphysiol.1994.sp003807 |
| format | Article |
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(0.1 mM) produces a similar increase in osmotic water flow when applied to the mucosal surface of the tissue. NEM-induced
water flow is sensitive to inhibitors of hormone-induced water flow, including serosal acidification, or exposure to quinidine
or cytoskeleton-disruptive drugs. NEM-induced water flow is additive with that induced by a submaximal, but not a maximal,
dose of vasopressin. The response to mucosal NEM is not reversed on removal of the reagent, but established NEM-induced water
flow can be inhibited by serosal acidification or quinidine. Like vasopressin, mucosal NEM induces the appearance of fusion
profiles and intramembranous particle aggregates (putative water channels) in the apical plasma membrane of the granular cells,
and the incidence of particle aggregates correlates with water flow. NEM does not cause an increase in intracellular cAMP.
Our data suggest that NEM stimulates transepithelial water flow by irreversibly activating cellular mechanisms normally triggered
by vasopressin, hence causing the insertion of water channels.</description><identifier>ISSN: 0958-0670</identifier><identifier>EISSN: 1469-445X</identifier><identifier>DOI: 10.1113/expphysiol.1994.sp003807</identifier><identifier>PMID: 7818866</identifier><language>eng</language><publisher>Cambridge: The Physiological Society</publisher><subject>Animals ; Biological and medical sciences ; Bufo marinus ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cell Membrane - ultrastructure ; Cell Membrane Permeability - drug effects ; Cyclic AMP - metabolism ; Epithelium - drug effects ; Epithelium - metabolism ; Epithelium - ultrastructure ; Ethylmaleimide - pharmacology ; Fundamental and applied biological sciences. Psychology ; Intracellular Fluid - drug effects ; Intracellular Fluid - metabolism ; Ion Transport - drug effects ; Maleimides - pharmacology ; Microscopy, Electron ; Mucous Membrane - drug effects ; Mucous Membrane - metabolism ; Mucous Membrane - ultrastructure ; Osmolar Concentration ; Sodium - metabolism ; Urinary Bladder - drug effects ; Urinary Bladder - metabolism ; Urinary Bladder - ultrastructure ; Vasopressins - antagonists & inhibitors ; Vasopressins - pharmacology ; Vertebrates: urinary system ; Water - metabolism</subject><ispartof>Experimental physiology, 1994-09, Vol.79 (5), p.775-795</ispartof><rights>1994 The Physiological Society</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4805-2e419608a3faea3512a60584aa34ae7501fe28e84ff57a40efc00e45f6b4e30b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1113%2Fexpphysiol.1994.sp003807$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1113%2Fexpphysiol.1994.sp003807$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4256867$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7818866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marples, D</creatorcontrib><creatorcontrib>Bourguet, J</creatorcontrib><creatorcontrib>Taylor, A</creatorcontrib><title>Activation of the vasopressin-sensitive water permeability pathway in the toad bladder by N-ethyl maleimide</title><title>Experimental physiology</title><addtitle>Exp Physiol</addtitle><description>Vasopressin stimulates transepithelial water flow in the toad urinary bladder. We report here that N-ethyl maleimide (NEM)
(0.1 mM) produces a similar increase in osmotic water flow when applied to the mucosal surface of the tissue. NEM-induced
water flow is sensitive to inhibitors of hormone-induced water flow, including serosal acidification, or exposure to quinidine
or cytoskeleton-disruptive drugs. NEM-induced water flow is additive with that induced by a submaximal, but not a maximal,
dose of vasopressin. The response to mucosal NEM is not reversed on removal of the reagent, but established NEM-induced water
flow can be inhibited by serosal acidification or quinidine. Like vasopressin, mucosal NEM induces the appearance of fusion
profiles and intramembranous particle aggregates (putative water channels) in the apical plasma membrane of the granular cells,
and the incidence of particle aggregates correlates with water flow. NEM does not cause an increase in intracellular cAMP.
Our data suggest that NEM stimulates transepithelial water flow by irreversibly activating cellular mechanisms normally triggered
by vasopressin, hence causing the insertion of water channels.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bufo marinus</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cyclic AMP - metabolism</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - metabolism</subject><subject>Epithelium - ultrastructure</subject><subject>Ethylmaleimide - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Intracellular Fluid - drug effects</subject><subject>Intracellular Fluid - metabolism</subject><subject>Ion Transport - drug effects</subject><subject>Maleimides - pharmacology</subject><subject>Microscopy, Electron</subject><subject>Mucous Membrane - drug effects</subject><subject>Mucous Membrane - metabolism</subject><subject>Mucous Membrane - ultrastructure</subject><subject>Osmolar Concentration</subject><subject>Sodium - metabolism</subject><subject>Urinary Bladder - drug effects</subject><subject>Urinary Bladder - metabolism</subject><subject>Urinary Bladder - ultrastructure</subject><subject>Vasopressins - antagonists & inhibitors</subject><subject>Vasopressins - pharmacology</subject><subject>Vertebrates: urinary system</subject><subject>Water - metabolism</subject><issn>0958-0670</issn><issn>1469-445X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1v1DAQhi0EKkvhJyD5gLhlsTf-yrFatRSpAg4gcbMmyZgYnA9sb5f8exJ2aa-cRpp53hn7IYRytuWcl-_w9zR1c_Jj2PKqEts0MVYapp-QDReqKoSQ356SDaukKZjS7Dl5kdIPxnjJjLggF9pwY5TakJ9XTfb3kP040NHR3CG9hzROEVPyQ5FwSH4BkB4hY6QTxh6h9sHnmU6QuyPM1A9_c3mEltYB2nYB65l-LDB3c6A9BPS9b_EleeYgJHx1rpfk6831l_1tcffp_Yf91V3RCMNksUPBK8UMlA4QSsl3oJg0AqAUgFoy7nBn0AjnpAbB0DWMoZBO1QJLVpeX5O1p7xTHXwdM2fY-NRgCDDgektWqKivB9AKaE9jEMaWIzk7R9xBny5ldPdtHz3b1bP95XqKvzzcOdY_tQ_Asdpm_Oc8hNRBchKHx6QETO6mMWtfsT9jRB5z_-7y9_ny7NnQltZaPH-789-7oI9pTKo2Nx7w8qrLSruAf0sOvcw</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Marples, D</creator><creator>Bourguet, J</creator><creator>Taylor, A</creator><general>The Physiological Society</general><general>Cambridge University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940901</creationdate><title>Activation of the vasopressin-sensitive water permeability pathway in the toad bladder by N-ethyl maleimide</title><author>Marples, D ; Bourguet, J ; Taylor, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4805-2e419608a3faea3512a60584aa34ae7501fe28e84ff57a40efc00e45f6b4e30b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bufo marinus</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Cyclic AMP - metabolism</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - metabolism</topic><topic>Epithelium - ultrastructure</topic><topic>Ethylmaleimide - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Intracellular Fluid - drug effects</topic><topic>Intracellular Fluid - metabolism</topic><topic>Ion Transport - drug effects</topic><topic>Maleimides - pharmacology</topic><topic>Microscopy, Electron</topic><topic>Mucous Membrane - drug effects</topic><topic>Mucous Membrane - metabolism</topic><topic>Mucous Membrane - ultrastructure</topic><topic>Osmolar Concentration</topic><topic>Sodium - metabolism</topic><topic>Urinary Bladder - drug effects</topic><topic>Urinary Bladder - metabolism</topic><topic>Urinary Bladder - ultrastructure</topic><topic>Vasopressins - antagonists & inhibitors</topic><topic>Vasopressins - pharmacology</topic><topic>Vertebrates: urinary system</topic><topic>Water - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marples, D</creatorcontrib><creatorcontrib>Bourguet, J</creatorcontrib><creatorcontrib>Taylor, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marples, D</au><au>Bourguet, J</au><au>Taylor, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of the vasopressin-sensitive water permeability pathway in the toad bladder by N-ethyl maleimide</atitle><jtitle>Experimental physiology</jtitle><addtitle>Exp Physiol</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>79</volume><issue>5</issue><spage>775</spage><epage>795</epage><pages>775-795</pages><issn>0958-0670</issn><eissn>1469-445X</eissn><abstract>Vasopressin stimulates transepithelial water flow in the toad urinary bladder. We report here that N-ethyl maleimide (NEM)
(0.1 mM) produces a similar increase in osmotic water flow when applied to the mucosal surface of the tissue. NEM-induced
water flow is sensitive to inhibitors of hormone-induced water flow, including serosal acidification, or exposure to quinidine
or cytoskeleton-disruptive drugs. NEM-induced water flow is additive with that induced by a submaximal, but not a maximal,
dose of vasopressin. The response to mucosal NEM is not reversed on removal of the reagent, but established NEM-induced water
flow can be inhibited by serosal acidification or quinidine. Like vasopressin, mucosal NEM induces the appearance of fusion
profiles and intramembranous particle aggregates (putative water channels) in the apical plasma membrane of the granular cells,
and the incidence of particle aggregates correlates with water flow. NEM does not cause an increase in intracellular cAMP.
Our data suggest that NEM stimulates transepithelial water flow by irreversibly activating cellular mechanisms normally triggered
by vasopressin, hence causing the insertion of water channels.</abstract><cop>Cambridge</cop><pub>The Physiological Society</pub><pmid>7818866</pmid><doi>10.1113/expphysiol.1994.sp003807</doi><tpages>21</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Bufo marinus Cell Membrane - drug effects Cell Membrane - metabolism Cell Membrane - ultrastructure Cell Membrane Permeability - drug effects Cyclic AMP - metabolism Epithelium - drug effects Epithelium - metabolism Epithelium - ultrastructure Ethylmaleimide - pharmacology Fundamental and applied biological sciences. Psychology Intracellular Fluid - drug effects Intracellular Fluid - metabolism Ion Transport - drug effects Maleimides - pharmacology Microscopy, Electron Mucous Membrane - drug effects Mucous Membrane - metabolism Mucous Membrane - ultrastructure Osmolar Concentration Sodium - metabolism Urinary Bladder - drug effects Urinary Bladder - metabolism Urinary Bladder - ultrastructure Vasopressins - antagonists & inhibitors Vasopressins - pharmacology Vertebrates: urinary system Water - metabolism |
| title | Activation of the vasopressin-sensitive water permeability pathway in the toad bladder by N-ethyl maleimide |
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