The adenosine triphosphate-pyrophosphate isotopic exchange reaction: A tool for determination of tryptophan

Quantitative determination of tryptophan at the picomole level is described, using the ATP-[ 32P] PP i isotopic exchange reaction catalyzed by tryptophanyl-tRNA synthetase. Sensitivity limits of 500 fmol were obtained. The presence of other amino acids at a 1000-fold excess over tryptophan did not i...

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Veröffentlicht in:	Anal. Biochem.; (United States) 1986-05, Vol.154 (2), p.618-623
Hauptverfasser:	Trezeguet, Véronique, Labouesse, Bernard
Format:	Artikel
Sprache:	eng
Schlagworte:	400303 - Organic Chemistry- Isotope Exchange & Isotope Separation- (-1987) 550201 - Biochemistry- Tracer Techniques Adenosine Triphosphate amino acid determination AMINO ACIDS Amino Acids - analysis Aminoacids, peptides. Hormones. Neuropeptides aminoacyl-tRNA synthetase Analytical, structural and metabolic biochemistry Applied sciences AROMATICS ATP AZAARENES AZOLES BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences CARBOXYLIC ACIDS CATALYSIS CHEMICAL ANALYSIS CHROMATOGRAPHY Chromatography, High Pressure Liquid DAYS LIVING RADIOISOTOPES Diphosphates Exact sciences and technology Fundamental and applied biological sciences. Psychology HETEROCYCLIC ACIDS HETEROCYCLIC COMPOUNDS INDOLES INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ISOTOPES ISOTOPIC EXCHANGE Kinetics LIGHT NUCLEI Microchemistry Models, Chemical NUCLEI NUCLEOTIDES ODD-ODD NUCLEI ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS Other techniques and industries OXYGEN COMPOUNDS PHOSPHORUS 32 PHOSPHORUS COMPOUNDS PHOSPHORUS ISOTOPES Phosphorus Radioisotopes protein Proteins PYROPHOSPHATES PYRROLES QUANTITATIVE CHEMICAL ANALYSIS RADIOISOTOPES SEPARATION PROCESSES Spectrometry, Fluorescence TRYPTOPHAN Tryptophan - analogs & derivatives Tryptophan - analysis Tryptophan-tRNA Ligase tryptophanyl-tRNA synthetase
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