A simplified technique for histologic analysis of central nervous system tissues using glycol-methacrylate plastic coupled with pre-embedding immunocytochemistry

Paraffin and some plastic embedding techniques will destroy many antigens routinely detected by immunocytochemistry performed on frozen tissue sections. However, morphologic quality is compromised to varying extents in frozen tissue, even with the use of cryoprotection. We report a simple glycol-met...

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Veröffentlicht in:Journal of neuroscience methods 1994-09, Vol.54 (1), p.23-29
Hauptverfasser: Abbott, Louise C, Conforti, Michael L, Isaacs, Krystyna R, Crawley, Jacqueline N, Sterchi, Diane
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Sprache:eng
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