Liquid Chromatography/Mass Spectrometry of Phospholipids using Electrospray Ionization
An improved technique for phospholipid molecular species analysis was developed using high-performance liquid chromatography/mass spectrometry with the electrospray interface. Using the 0.5% ammonium hydroxide in a water-methanol-hexane mixture and a C-18 column, complex mixtures of phospholipid mol...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1994-11, Vol.66 (22), p.3977-3982 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Kim, Hee-Yong Wang, Tao-Chin L Ma, Yee-Chung |
| description | An improved technique for phospholipid molecular species analysis was developed using high-performance liquid chromatography/mass spectrometry with the electrospray interface. Using the 0.5% ammonium hydroxide in a water-methanol-hexane mixture and a C-18 column, complex mixtures of phospholipid molecular species were separated and detected mainly as protonated or natriated molecular species. The response was linear over 2 orders of magnitude, allowing quantification of each molecular species. In comparison to the existing LC/MS techniques, marked improvement in sensitivity was observed. The present quantification limit is approximately 0.5 pmol before split (5 fmol after 1/100 split). The relative responses were more dependent on the head group identity rather than fatty acyl composition within a phospholipid class. In general, phosphatidylcholine (PC) species are most sensitively detected followed by phosphatidylethanolamine (PE) species. The sensitivity of phosphatidylserine (PS) in the positive ion mode is approximately 20 times less in comparison to PC under our condition. |
| doi_str_mv | 10.1021/ac00094a020 |
| format | Article |
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Using the 0.5% ammonium hydroxide in a water-methanol-hexane mixture and a C-18 column, complex mixtures of phospholipid molecular species were separated and detected mainly as protonated or natriated molecular species. The response was linear over 2 orders of magnitude, allowing quantification of each molecular species. In comparison to the existing LC/MS techniques, marked improvement in sensitivity was observed. The present quantification limit is approximately 0.5 pmol before split (5 fmol after 1/100 split). The relative responses were more dependent on the head group identity rather than fatty acyl composition within a phospholipid class. In general, phosphatidylcholine (PC) species are most sensitively detected followed by phosphatidylethanolamine (PE) species. The sensitivity of phosphatidylserine (PS) in the positive ion mode is approximately 20 times less in comparison to PC under our condition.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac00094a020</identifier><identifier>PMID: 7810900</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Biochemistry ; Chromatography, High Pressure Liquid ; Docosahexaenoic Acids - chemistry ; Glioma - metabolism ; Glioma - pathology ; Ions ; Mass Spectrometry ; Phosphatidylcholines - analysis ; Phosphatidylethanolamines - analysis ; Phosphatidylserines - analysis ; Rats ; Reproducibility of Results ; Scientific imaging ; Tumor Cells, Cultured</subject><ispartof>Analytical chemistry (Washington), 1994-11, Vol.66 (22), p.3977-3982</ispartof><rights>Copyright American Chemical Society Nov 15, 1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a447t-f4bebfe76d8c07e6e3f96774c72e5d3cb354fab390979d6092ea8a5ee0d406fe3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac00094a020$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac00094a020$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56717,56767</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7810900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hee-Yong</creatorcontrib><creatorcontrib>Wang, Tao-Chin L</creatorcontrib><creatorcontrib>Ma, Yee-Chung</creatorcontrib><title>Liquid Chromatography/Mass Spectrometry of Phospholipids using Electrospray Ionization</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. 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The sensitivity of phosphatidylserine (PS) in the positive ion mode is approximately 20 times less in comparison to PC under our condition.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Docosahexaenoic Acids - chemistry</subject><subject>Glioma - metabolism</subject><subject>Glioma - pathology</subject><subject>Ions</subject><subject>Mass Spectrometry</subject><subject>Phosphatidylcholines - analysis</subject><subject>Phosphatidylethanolamines - analysis</subject><subject>Phosphatidylserines - analysis</subject><subject>Rats</subject><subject>Reproducibility of Results</subject><subject>Scientific imaging</subject><subject>Tumor Cells, Cultured</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM1P3DAQxa2qFSy0p54rRT2UA0oZf8SOj9VCt0hbFQnaQy-Wk0xYQzYOdiKx_PU13RVFqKeR5v00780j5D2FzxQYPbE1AGhhgcErMqMFg1yWJXtNZmnPc6YA9slBjDcAlAKVe2RPlRQ0wIz8Wrq7yTXZfBX82o7-OthhtTn5bmPMLgesx7TGMWwy32YXKx-Hle_c4JqYTdH119lZ95eJQ7Cb7Nz37sGOzvdvyZvWdhHf7eYh-fn17Gr-LV_-WJzPvyxzK4Qa81ZUWLWoZFPWoFAib7VUStSKYdHwuuKFaG3FNWilGwmaoS1tgQiNANkiPySftneH4O8mjKNZu1hj19ke_RSNkpoxwVUCP74Ab_wU-pTNMKpKkVx1go63UJ0-igFbMwS3tmFjKJjHqs2zqhP9YXdyqtbYPLG7bpOeb3UXR7x_km24NVJxVZiri0uzOFXzU774bcrEH215W8d_6f7n_Ad6qZWi</recordid><startdate>19941115</startdate><enddate>19941115</enddate><creator>Kim, Hee-Yong</creator><creator>Wang, Tao-Chin L</creator><creator>Ma, Yee-Chung</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19941115</creationdate><title>Liquid Chromatography/Mass Spectrometry of Phospholipids using Electrospray Ionization</title><author>Kim, Hee-Yong ; Wang, Tao-Chin L ; Ma, Yee-Chung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a447t-f4bebfe76d8c07e6e3f96774c72e5d3cb354fab390979d6092ea8a5ee0d406fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Docosahexaenoic Acids - chemistry</topic><topic>Glioma - metabolism</topic><topic>Glioma - pathology</topic><topic>Ions</topic><topic>Mass Spectrometry</topic><topic>Phosphatidylcholines - analysis</topic><topic>Phosphatidylethanolamines - analysis</topic><topic>Phosphatidylserines - analysis</topic><topic>Rats</topic><topic>Reproducibility of Results</topic><topic>Scientific imaging</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hee-Yong</creatorcontrib><creatorcontrib>Wang, Tao-Chin L</creatorcontrib><creatorcontrib>Ma, Yee-Chung</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hee-Yong</au><au>Wang, Tao-Chin L</au><au>Ma, Yee-Chung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid Chromatography/Mass Spectrometry of Phospholipids using Electrospray Ionization</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1994-11-15</date><risdate>1994</risdate><volume>66</volume><issue>22</issue><spage>3977</spage><epage>3982</epage><pages>3977-3982</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>An improved technique for phospholipid molecular species analysis was developed using high-performance liquid chromatography/mass spectrometry with the electrospray interface. Using the 0.5% ammonium hydroxide in a water-methanol-hexane mixture and a C-18 column, complex mixtures of phospholipid molecular species were separated and detected mainly as protonated or natriated molecular species. The response was linear over 2 orders of magnitude, allowing quantification of each molecular species. In comparison to the existing LC/MS techniques, marked improvement in sensitivity was observed. The present quantification limit is approximately 0.5 pmol before split (5 fmol after 1/100 split). The relative responses were more dependent on the head group identity rather than fatty acyl composition within a phospholipid class. In general, phosphatidylcholine (PC) species are most sensitively detected followed by phosphatidylethanolamine (PE) species. The sensitivity of phosphatidylserine (PS) in the positive ion mode is approximately 20 times less in comparison to PC under our condition.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>7810900</pmid><doi>10.1021/ac00094a020</doi><tpages>6</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 1994-11, Vol.66 (22), p.3977-3982 |
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| source | MEDLINE; ACS Publications |
| subjects | Animals Biochemistry Chromatography, High Pressure Liquid Docosahexaenoic Acids - chemistry Glioma - metabolism Glioma - pathology Ions Mass Spectrometry Phosphatidylcholines - analysis Phosphatidylethanolamines - analysis Phosphatidylserines - analysis Rats Reproducibility of Results Scientific imaging Tumor Cells, Cultured |
| title | Liquid Chromatography/Mass Spectrometry of Phospholipids using Electrospray Ionization |
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