Autologous red cell agglutination test for antibodies to feline immunodeficiency virus
The T-lymphotropic lentivirus, feline immunodeficiency virus (FIV) is now recognised as a major viral pathogen affecting domestic cat populations worldwide. A rapid, autologous red cell agglutination test for antibodies to FIV has been developed. A synthetic peptide analog corresponding to the immun...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 1994-09, Vol.42 (3-4), p.253-263 |
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| creator | Wilson, K.M. Robinson, W.F. Mitchelhill, K.I. Bundesen, P.G. Kemp, B.E. |
| description | The T-lymphotropic lentivirus, feline immunodeficiency virus (FIV) is now recognised as a major viral pathogen affecting domestic cat populations worldwide. A rapid, autologous red cell agglutination test for antibodies to FIV has been developed. A synthetic peptide analog corresponding to the immunodominant epitope within the FIV transmembrane glycoprotein gp40 residues (680–715) KVEAMEKFLYTAFAMQELGC (Acm)NQNQFFK(BrAc)KIPLELWTR was conjugated to an anti-feline erythrocyte antibody using a thio-ether linkage. Within 3 min of adding this reagent to 20 μl of whole blood, circulating antibody to the peptide epitope caused agglutination of the red blood cells. The performance of this simple test is comparable with the two commercially available enzyme immunoassay (EIA) kits and an EIA based on this peptide. A variant of the gp40 (680–715) peptide corresponding to the FIV, PPR strain gp40 (678–716) sequence was also synthesised and no difference in reactivity was observed in an EIA on 211 seropositive samples, indicating that the peptide-based test may be applicable to other known strains of the virus. |
| doi_str_mv | 10.1016/0165-2427(94)90071-X |
| format | Article |
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A rapid, autologous red cell agglutination test for antibodies to FIV has been developed. A synthetic peptide analog corresponding to the immunodominant epitope within the FIV transmembrane glycoprotein gp40 residues (680–715) KVEAMEKFLYTAFAMQELGC (Acm)NQNQFFK(BrAc)KIPLELWTR was conjugated to an anti-feline erythrocyte antibody using a thio-ether linkage. Within 3 min of adding this reagent to 20 μl of whole blood, circulating antibody to the peptide epitope caused agglutination of the red blood cells. The performance of this simple test is comparable with the two commercially available enzyme immunoassay (EIA) kits and an EIA based on this peptide. A variant of the gp40 (680–715) peptide corresponding to the FIV, PPR strain gp40 (678–716) sequence was also synthesised and no difference in reactivity was observed in an EIA on 211 seropositive samples, indicating that the peptide-based test may be applicable to other known strains of the virus.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/0165-2427(94)90071-X</identifier><identifier>PMID: 7810059</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>AGGLUTINATION TESTS ; AIDS/HIV ; Amino Acid Sequence ; Animals ; ANTIBODIES ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - analysis ; ANTICORPS ; ANTICUERPOS ; Biological and medical sciences ; CATS ; CHAT ; Enzyme-Linked Immunosorbent Assay - veterinary ; Erythrocytes - immunology ; Feline Acquired Immunodeficiency Syndrome - diagnosis ; feline immunodeficiency virus ; Fundamental and applied biological sciences. Psychology ; GATO ; Hemagglutination - immunology ; Hemagglutination Tests - veterinary ; Immunodeficiency Virus, Feline - immunology ; Immunodominant Epitopes - immunology ; LENTIVIRINAE ; Microbiology ; Molecular Sequence Data ; REACCIONES DE AGLUTINACION ; REACTION D'AGGLUTINATION ; Techniques used in virology ; Viral Envelope Proteins - chemical synthesis ; Viral Envelope Proteins - immunology ; Virology</subject><ispartof>Veterinary immunology and immunopathology, 1994-09, Vol.42 (3-4), p.253-263</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-756c2ca7eab716e6082eab506a0fde3fee24248d94cf491ef9ba68286a34710a3</citedby><cites>FETCH-LOGICAL-c436t-756c2ca7eab716e6082eab506a0fde3fee24248d94cf491ef9ba68286a34710a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/016524279490071X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4236196$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7810059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wilson, K.M.</creatorcontrib><creatorcontrib>Robinson, W.F.</creatorcontrib><creatorcontrib>Mitchelhill, K.I.</creatorcontrib><creatorcontrib>Bundesen, P.G.</creatorcontrib><creatorcontrib>Kemp, B.E.</creatorcontrib><title>Autologous red cell agglutination test for antibodies to feline immunodeficiency virus</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>The T-lymphotropic lentivirus, feline immunodeficiency virus (FIV) is now recognised as a major viral pathogen affecting domestic cat populations worldwide. A rapid, autologous red cell agglutination test for antibodies to FIV has been developed. A synthetic peptide analog corresponding to the immunodominant epitope within the FIV transmembrane glycoprotein gp40 residues (680–715) KVEAMEKFLYTAFAMQELGC (Acm)NQNQFFK(BrAc)KIPLELWTR was conjugated to an anti-feline erythrocyte antibody using a thio-ether linkage. Within 3 min of adding this reagent to 20 μl of whole blood, circulating antibody to the peptide epitope caused agglutination of the red blood cells. The performance of this simple test is comparable with the two commercially available enzyme immunoassay (EIA) kits and an EIA based on this peptide. A variant of the gp40 (680–715) peptide corresponding to the FIV, PPR strain gp40 (678–716) sequence was also synthesised and no difference in reactivity was observed in an EIA on 211 seropositive samples, indicating that the peptide-based test may be applicable to other known strains of the virus.</description><subject>AGGLUTINATION TESTS</subject><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>ANTIBODIES</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral - analysis</subject><subject>ANTICORPS</subject><subject>ANTICUERPOS</subject><subject>Biological and medical sciences</subject><subject>CATS</subject><subject>CHAT</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Erythrocytes - immunology</subject><subject>Feline Acquired Immunodeficiency Syndrome - diagnosis</subject><subject>feline immunodeficiency virus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GATO</subject><subject>Hemagglutination - immunology</subject><subject>Hemagglutination Tests - veterinary</subject><subject>Immunodeficiency Virus, Feline - immunology</subject><subject>Immunodominant Epitopes - immunology</subject><subject>LENTIVIRINAE</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>REACCIONES DE AGLUTINACION</subject><subject>REACTION D'AGGLUTINATION</subject><subject>Techniques used in virology</subject><subject>Viral Envelope Proteins - chemical synthesis</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Virology</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9rFTEUxUNR6mvtFxALWYjoYjTJZPJnI5RSq_BoNyrdhbzMzSMyM6lJptBvb6bv8ZbtIuTC-d3DuQeh95R8oYSKr_V1DeNMftL8syZE0ubuCK2okm3Dupa_QqsD8gad5PyXENJppY7RsVR0mVfoz8Vc4hC3cc44QY8dDAO22-0wlzDZEuKEC-SCfUzYTiVsYh8g4xKxhyFMgMM4zlPswQcXYHKP-CGkOb9Fr70dMpzt_1P0-_vVr8sfzfr2-uflxbpxvBWlkZ1wzFkJdiOpAEEUq2NHhCW-h9YD1PBc9Zo7zzUFrzdWKKaEbbmkxLan6OPO9z7Ff3MNasaQlxvsBPUkI4VmlCvxIkiF7JhoaQX5DnQp5pzAm_sURpseDSVm6d0spZqlVKO5eerd3NW1873_vBmhPyzti676h71us7ODT3ZyIR8wzlpB9RLz3Q7zNhq7TRW5WeuuWhBSxW87EWqjDwGSyU-dQx8SuGL6GJ4P-R_R36fZ</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Wilson, K.M.</creator><creator>Robinson, W.F.</creator><creator>Mitchelhill, K.I.</creator><creator>Bundesen, P.G.</creator><creator>Kemp, B.E.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940901</creationdate><title>Autologous red cell agglutination test for antibodies to feline immunodeficiency virus</title><author>Wilson, K.M. ; Robinson, W.F. ; Mitchelhill, K.I. ; Bundesen, P.G. ; Kemp, B.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-756c2ca7eab716e6082eab506a0fde3fee24248d94cf491ef9ba68286a34710a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>AGGLUTINATION TESTS</topic><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>ANTIBODIES</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral - analysis</topic><topic>ANTICORPS</topic><topic>ANTICUERPOS</topic><topic>Biological and medical sciences</topic><topic>CATS</topic><topic>CHAT</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Erythrocytes - immunology</topic><topic>Feline Acquired Immunodeficiency Syndrome - diagnosis</topic><topic>feline immunodeficiency virus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GATO</topic><topic>Hemagglutination - immunology</topic><topic>Hemagglutination Tests - veterinary</topic><topic>Immunodeficiency Virus, Feline - immunology</topic><topic>Immunodominant Epitopes - immunology</topic><topic>LENTIVIRINAE</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>REACCIONES DE AGLUTINACION</topic><topic>REACTION D'AGGLUTINATION</topic><topic>Techniques used in virology</topic><topic>Viral Envelope Proteins - chemical synthesis</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilson, K.M.</creatorcontrib><creatorcontrib>Robinson, W.F.</creatorcontrib><creatorcontrib>Mitchelhill, K.I.</creatorcontrib><creatorcontrib>Bundesen, P.G.</creatorcontrib><creatorcontrib>Kemp, B.E.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wilson, K.M.</au><au>Robinson, W.F.</au><au>Mitchelhill, K.I.</au><au>Bundesen, P.G.</au><au>Kemp, B.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autologous red cell agglutination test for antibodies to feline immunodeficiency virus</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>42</volume><issue>3-4</issue><spage>253</spage><epage>263</epage><pages>253-263</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>The T-lymphotropic lentivirus, feline immunodeficiency virus (FIV) is now recognised as a major viral pathogen affecting domestic cat populations worldwide. A rapid, autologous red cell agglutination test for antibodies to FIV has been developed. A synthetic peptide analog corresponding to the immunodominant epitope within the FIV transmembrane glycoprotein gp40 residues (680–715) KVEAMEKFLYTAFAMQELGC (Acm)NQNQFFK(BrAc)KIPLELWTR was conjugated to an anti-feline erythrocyte antibody using a thio-ether linkage. Within 3 min of adding this reagent to 20 μl of whole blood, circulating antibody to the peptide epitope caused agglutination of the red blood cells. The performance of this simple test is comparable with the two commercially available enzyme immunoassay (EIA) kits and an EIA based on this peptide. A variant of the gp40 (680–715) peptide corresponding to the FIV, PPR strain gp40 (678–716) sequence was also synthesised and no difference in reactivity was observed in an EIA on 211 seropositive samples, indicating that the peptide-based test may be applicable to other known strains of the virus.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>7810059</pmid><doi>10.1016/0165-2427(94)90071-X</doi><tpages>11</tpages></addata></record> |
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| ispartof | Veterinary immunology and immunopathology, 1994-09, Vol.42 (3-4), p.253-263 |
| issn | 0165-2427 1873-2534 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | AGGLUTINATION TESTS AIDS/HIV Amino Acid Sequence Animals ANTIBODIES Antibodies, Monoclonal - immunology Antibodies, Viral - analysis ANTICORPS ANTICUERPOS Biological and medical sciences CATS CHAT Enzyme-Linked Immunosorbent Assay - veterinary Erythrocytes - immunology Feline Acquired Immunodeficiency Syndrome - diagnosis feline immunodeficiency virus Fundamental and applied biological sciences. Psychology GATO Hemagglutination - immunology Hemagglutination Tests - veterinary Immunodeficiency Virus, Feline - immunology Immunodominant Epitopes - immunology LENTIVIRINAE Microbiology Molecular Sequence Data REACCIONES DE AGLUTINACION REACTION D'AGGLUTINATION Techniques used in virology Viral Envelope Proteins - chemical synthesis Viral Envelope Proteins - immunology Virology |
| title | Autologous red cell agglutination test for antibodies to feline immunodeficiency virus |
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