Interaction of cAMP with the Cell-Surface Receptor Induces Cell-Type-Specific mRNA Accumulation in Dictyostelium discoideum
The accumulation of many postaggregative mRNA species in Dictyostelium discoideum is dependent upon the continuous presence of elevated levels of cAMP. We have analyzed the cyclic nucleotide specificity of this requirement and show that it is similar to that of the cell-surface receptor and distinct...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1986-07, Vol.83 (13), p.4819-4823 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4823 |
|---|---|
| container_issue | 13 |
| container_start_page | 4819 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 83 |
| creator | Oyama, Masakazu Blumberg, Daphne D. |
| description | The accumulation of many postaggregative mRNA species in Dictyostelium discoideum is dependent upon the continuous presence of elevated levels of cAMP. We have analyzed the cyclic nucleotide specificity of this requirement and show that it is similar to that of the cell-surface receptor and distinct from the specificity displayed by the cAMP-dependent protein kinase. The same specificity is displayed for the accumulation of two classes of prespore mRNAs (class I, early; class II, late) and a prestalk mRNA and for the shutoff of a growth-phase mRNA. Under conditions in which cAMP phosphodiesterase activity is competitively inhibited, half-maximal accumulation of prestalk mRNA can be obtained at cAMP concentrations of 320-520 nM, whereas a higher concentration, 1-2 μ M, is required for half-maximal accumulation of the prespore mRNAs and shutoff of the growth-phase mRNA. These effects of cAMP and its analogues on gene expression have been obtained under conditions in which cAMP-mediated activation of adenylate cyclase is completely inhibited. We conclude that cAMP acts to stimulate postaggregative gene expression by interacting at the cell-surface receptor. |
| doi_str_mv | 10.1073/pnas.83.13.4819 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_76921262</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>27599</jstor_id><sourcerecordid>27599</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4059-c8cffdd002d856e2cb61eff90678d5dc4ba91fa59159f4ab36a4aa294f654bbf3</originalsourceid><addsrcrecordid>eNp9kctv1DAYxC0EKkvhjIQE8gHBKVu_ktgHDqstj5XKQ205W45js66SOLUdyop_Hi8brcqFkw_zm_k8GgCeY7TEqKZn46DiktMlpkvGsXgAFhgJXFRMoIdggRCpC84IewyexHiDEBIlRyfghCLMSkwW4PdmSCYonZwfoLdQrz5_g3cubWHaGrg2XVdcTcEqbeCl0WZMPsDN0E7axIN6vRtNcTUa7azTsL_8soIrrad-6tTfTDfAc6fTzsdkOjf1sHVRe9eaqX8KHlnVRfNsfk_B9w_vr9efiouvHzfr1UWhGSpFobm2tm1zl5aXlSG6qbCxVqCq5m3ZatYoga0qBS6FZaqhlWJKEcFsVbKmsfQUvDvkjlPTm1abIQXVyTG4XoWd9MrJf5XBbeUP_1NSQjml2f9m9gd_O5mYZJ875PJqMH6Ksq4EwaQiGTw7gDr4GIOxxxsYyf1ccj-X5FRiKvdzZcfL-1878vM-WX896ypq1dmgBu3iEeN1xZlgGXs1Y_v8o3r_ztv_AtJOXZfMr5TJFwfyJuatjyipSyHoH0fVwfk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76921262</pqid></control><display><type>article</type><title>Interaction of cAMP with the Cell-Surface Receptor Induces Cell-Type-Specific mRNA Accumulation in Dictyostelium discoideum</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Oyama, Masakazu ; Blumberg, Daphne D.</creator><creatorcontrib>Oyama, Masakazu ; Blumberg, Daphne D.</creatorcontrib><description>The accumulation of many postaggregative mRNA species in Dictyostelium discoideum is dependent upon the continuous presence of elevated levels of cAMP. We have analyzed the cyclic nucleotide specificity of this requirement and show that it is similar to that of the cell-surface receptor and distinct from the specificity displayed by the cAMP-dependent protein kinase. The same specificity is displayed for the accumulation of two classes of prespore mRNAs (class I, early; class II, late) and a prestalk mRNA and for the shutoff of a growth-phase mRNA. Under conditions in which cAMP phosphodiesterase activity is competitively inhibited, half-maximal accumulation of prestalk mRNA can be obtained at cAMP concentrations of 320-520 nM, whereas a higher concentration, 1-2 μ M, is required for half-maximal accumulation of the prespore mRNAs and shutoff of the growth-phase mRNA. These effects of cAMP and its analogues on gene expression have been obtained under conditions in which cAMP-mediated activation of adenylate cyclase is completely inhibited. We conclude that cAMP acts to stimulate postaggregative gene expression by interacting at the cell-surface receptor.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.83.13.4819</identifier><identifier>PMID: 3014512</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Adenylyl Cyclases - physiology ; Aggregation ; Albumins ; Biological and medical sciences ; Carrier proteins ; Cell aggregates ; Cell Differentiation ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cells ; Cellular receptors ; Cyclic AMP - physiology ; Developmental biology ; Dictyostelium - cytology ; Dictyostelium - physiology ; Enzyme Activation ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation ; Messenger RNA ; Molecular and cellular biology ; Receptors ; Receptors, Cell Surface - physiology ; Receptors, Cyclic AMP - physiology ; RNA, Messenger - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1986-07, Vol.83 (13), p.4819-4823</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4059-c8cffdd002d856e2cb61eff90678d5dc4ba91fa59159f4ab36a4aa294f654bbf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/83/13.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/27599$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/27599$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8768494$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3014512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oyama, Masakazu</creatorcontrib><creatorcontrib>Blumberg, Daphne D.</creatorcontrib><title>Interaction of cAMP with the Cell-Surface Receptor Induces Cell-Type-Specific mRNA Accumulation in Dictyostelium discoideum</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The accumulation of many postaggregative mRNA species in Dictyostelium discoideum is dependent upon the continuous presence of elevated levels of cAMP. We have analyzed the cyclic nucleotide specificity of this requirement and show that it is similar to that of the cell-surface receptor and distinct from the specificity displayed by the cAMP-dependent protein kinase. The same specificity is displayed for the accumulation of two classes of prespore mRNAs (class I, early; class II, late) and a prestalk mRNA and for the shutoff of a growth-phase mRNA. Under conditions in which cAMP phosphodiesterase activity is competitively inhibited, half-maximal accumulation of prestalk mRNA can be obtained at cAMP concentrations of 320-520 nM, whereas a higher concentration, 1-2 μ M, is required for half-maximal accumulation of the prespore mRNAs and shutoff of the growth-phase mRNA. These effects of cAMP and its analogues on gene expression have been obtained under conditions in which cAMP-mediated activation of adenylate cyclase is completely inhibited. We conclude that cAMP acts to stimulate postaggregative gene expression by interacting at the cell-surface receptor.</description><subject>Adenylyl Cyclases - physiology</subject><subject>Aggregation</subject><subject>Albumins</subject><subject>Biological and medical sciences</subject><subject>Carrier proteins</subject><subject>Cell aggregates</subject><subject>Cell Differentiation</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cells</subject><subject>Cellular receptors</subject><subject>Cyclic AMP - physiology</subject><subject>Developmental biology</subject><subject>Dictyostelium - cytology</subject><subject>Dictyostelium - physiology</subject><subject>Enzyme Activation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Messenger RNA</subject><subject>Molecular and cellular biology</subject><subject>Receptors</subject><subject>Receptors, Cell Surface - physiology</subject><subject>Receptors, Cyclic AMP - physiology</subject><subject>RNA, Messenger - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctv1DAYxC0EKkvhjIQE8gHBKVu_ktgHDqstj5XKQ205W45js66SOLUdyop_Hi8brcqFkw_zm_k8GgCeY7TEqKZn46DiktMlpkvGsXgAFhgJXFRMoIdggRCpC84IewyexHiDEBIlRyfghCLMSkwW4PdmSCYonZwfoLdQrz5_g3cubWHaGrg2XVdcTcEqbeCl0WZMPsDN0E7axIN6vRtNcTUa7azTsL_8soIrrad-6tTfTDfAc6fTzsdkOjf1sHVRe9eaqX8KHlnVRfNsfk_B9w_vr9efiouvHzfr1UWhGSpFobm2tm1zl5aXlSG6qbCxVqCq5m3ZatYoga0qBS6FZaqhlWJKEcFsVbKmsfQUvDvkjlPTm1abIQXVyTG4XoWd9MrJf5XBbeUP_1NSQjml2f9m9gd_O5mYZJ875PJqMH6Ksq4EwaQiGTw7gDr4GIOxxxsYyf1ccj-X5FRiKvdzZcfL-1878vM-WX896ypq1dmgBu3iEeN1xZlgGXs1Y_v8o3r_ztv_AtJOXZfMr5TJFwfyJuatjyipSyHoH0fVwfk</recordid><startdate>19860701</startdate><enddate>19860701</enddate><creator>Oyama, Masakazu</creator><creator>Blumberg, Daphne D.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19860701</creationdate><title>Interaction of cAMP with the Cell-Surface Receptor Induces Cell-Type-Specific mRNA Accumulation in Dictyostelium discoideum</title><author>Oyama, Masakazu ; Blumberg, Daphne D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4059-c8cffdd002d856e2cb61eff90678d5dc4ba91fa59159f4ab36a4aa294f654bbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adenylyl Cyclases - physiology</topic><topic>Aggregation</topic><topic>Albumins</topic><topic>Biological and medical sciences</topic><topic>Carrier proteins</topic><topic>Cell aggregates</topic><topic>Cell Differentiation</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cells</topic><topic>Cellular receptors</topic><topic>Cyclic AMP - physiology</topic><topic>Developmental biology</topic><topic>Dictyostelium - cytology</topic><topic>Dictyostelium - physiology</topic><topic>Enzyme Activation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Messenger RNA</topic><topic>Molecular and cellular biology</topic><topic>Receptors</topic><topic>Receptors, Cell Surface - physiology</topic><topic>Receptors, Cyclic AMP - physiology</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oyama, Masakazu</creatorcontrib><creatorcontrib>Blumberg, Daphne D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oyama, Masakazu</au><au>Blumberg, Daphne D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of cAMP with the Cell-Surface Receptor Induces Cell-Type-Specific mRNA Accumulation in Dictyostelium discoideum</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1986-07-01</date><risdate>1986</risdate><volume>83</volume><issue>13</issue><spage>4819</spage><epage>4823</epage><pages>4819-4823</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The accumulation of many postaggregative mRNA species in Dictyostelium discoideum is dependent upon the continuous presence of elevated levels of cAMP. We have analyzed the cyclic nucleotide specificity of this requirement and show that it is similar to that of the cell-surface receptor and distinct from the specificity displayed by the cAMP-dependent protein kinase. The same specificity is displayed for the accumulation of two classes of prespore mRNAs (class I, early; class II, late) and a prestalk mRNA and for the shutoff of a growth-phase mRNA. Under conditions in which cAMP phosphodiesterase activity is competitively inhibited, half-maximal accumulation of prestalk mRNA can be obtained at cAMP concentrations of 320-520 nM, whereas a higher concentration, 1-2 μ M, is required for half-maximal accumulation of the prespore mRNAs and shutoff of the growth-phase mRNA. These effects of cAMP and its analogues on gene expression have been obtained under conditions in which cAMP-mediated activation of adenylate cyclase is completely inhibited. We conclude that cAMP acts to stimulate postaggregative gene expression by interacting at the cell-surface receptor.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3014512</pmid><doi>10.1073/pnas.83.13.4819</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1986-07, Vol.83 (13), p.4819-4823 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76921262 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adenylyl Cyclases - physiology Aggregation Albumins Biological and medical sciences Carrier proteins Cell aggregates Cell Differentiation Cell differentiation, maturation, development, hematopoiesis Cell physiology Cells Cellular receptors Cyclic AMP - physiology Developmental biology Dictyostelium - cytology Dictyostelium - physiology Enzyme Activation Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation Messenger RNA Molecular and cellular biology Receptors Receptors, Cell Surface - physiology Receptors, Cyclic AMP - physiology RNA, Messenger - genetics |
| title | Interaction of cAMP with the Cell-Surface Receptor Induces Cell-Type-Specific mRNA Accumulation in Dictyostelium discoideum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T09%3A34%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Interaction%20of%20cAMP%20with%20the%20Cell-Surface%20Receptor%20Induces%20Cell-Type-Specific%20mRNA%20Accumulation%20in%20Dictyostelium%20discoideum&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Oyama,%20Masakazu&rft.date=1986-07-01&rft.volume=83&rft.issue=13&rft.spage=4819&rft.epage=4823&rft.pages=4819-4823&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.83.13.4819&rft_dat=%3Cjstor_proqu%3E27599%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76921262&rft_id=info:pmid/3014512&rft_jstor_id=27599&rfr_iscdi=true |