Characterizing “difficult” acute leukemias. a combined electron microscopic and immunological marker study
The techniques of transmission electron microscopy (TEM), including ultrastructural myeloperoxidase cytochemistry (MPO), and immunological marker analysis, have been used to classify 58 “difficult” cases of acute leukemia where a precise diagnosis could not be made on the basis of conventional light...
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| Veröffentlicht in: | Pathology 1986, Vol.18 (1), p.99-110 |
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| container_end_page | 110 |
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| container_title | Pathology |
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| creator | Hewson, John W. Bradstock, K.F. Kerr, A. Rose, R.G. |
| description | The techniques of transmission electron microscopy (TEM), including ultrastructural myeloperoxidase cytochemistry (MPO), and immunological marker analysis, have been used to classify 58 “difficult” cases of acute leukemia where a precise diagnosis could not be made on the basis of conventional light microscopy and cytochemistry. TEM with MPO proved most valuable in characterizing 15 cases of acute myeloid leukemia and its variants, as well as defining complex cellular subpopulations in 11 cases of chronic myeloid leukemia in blast crisis. Immunological marker studies provided conclusive evidence of lymphoid differentiation in 18 cases of acute lymphoblastic leukemia and related disorders. In addition, the combined techniques were used to document 14 cases of terminal transferase-positive acute myeloid leukemia. This study demonstrates that these 2 techniques provide overlapping and complementary information for accurate diagnosis and classification of morphologically difficult hematological malignancies. |
| doi_str_mv | 10.3109/00313028609090835 |
| format | Article |
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TEM with MPO proved most valuable in characterizing 15 cases of acute myeloid leukemia and its variants, as well as defining complex cellular subpopulations in 11 cases of chronic myeloid leukemia in blast crisis. Immunological marker studies provided conclusive evidence of lymphoid differentiation in 18 cases of acute lymphoblastic leukemia and related disorders. In addition, the combined techniques were used to document 14 cases of terminal transferase-positive acute myeloid leukemia. This study demonstrates that these 2 techniques provide overlapping and complementary information for accurate diagnosis and classification of morphologically difficult hematological malignancies.</description><identifier>ISSN: 0031-3025</identifier><identifier>EISSN: 1465-3931</identifier><identifier>DOI: 10.3109/00313028609090835</identifier><identifier>PMID: 3014425</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Acute leukemia ; Adolescent ; Adult ; Aged ; Antigens, Neoplasm - analysis ; Antigens, Surface - analysis ; Child ; DNA Nucleotidylexotransferase - metabolism ; Female ; Humans ; Infant ; Leukemia - classification ; Leukemia - immunology ; Leukemia - ultrastructure ; leukemia antigens ; Male ; Microscopy, Electron ; Middle Aged ; monoclonal antibodies ; myeloperoxidase ; Peroxidase - metabolism ; terminal transferase ; ultrastructure</subject><ispartof>Pathology, 1986, Vol.18 (1), p.99-110</ispartof><rights>1986 Royal College of Pathologists of Australasia</rights><rights>1986 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1986</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-89b42612e6fc48356219ca4884dbd20750250752a98315c9837df8b9a5f6e4313</citedby><cites>FETCH-LOGICAL-c400t-89b42612e6fc48356219ca4884dbd20750250752a98315c9837df8b9a5f6e4313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/00313028609090835$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/00313028609090835$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4023,27922,27923,27924,61220,61401</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3014425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hewson, John W.</creatorcontrib><creatorcontrib>Bradstock, K.F.</creatorcontrib><creatorcontrib>Kerr, A.</creatorcontrib><creatorcontrib>Rose, R.G.</creatorcontrib><title>Characterizing “difficult” acute leukemias. a combined electron microscopic and immunological marker study</title><title>Pathology</title><addtitle>Pathology</addtitle><description>The techniques of transmission electron microscopy (TEM), including ultrastructural myeloperoxidase cytochemistry (MPO), and immunological marker analysis, have been used to classify 58 “difficult” cases of acute leukemia where a precise diagnosis could not be made on the basis of conventional light microscopy and cytochemistry. TEM with MPO proved most valuable in characterizing 15 cases of acute myeloid leukemia and its variants, as well as defining complex cellular subpopulations in 11 cases of chronic myeloid leukemia in blast crisis. Immunological marker studies provided conclusive evidence of lymphoid differentiation in 18 cases of acute lymphoblastic leukemia and related disorders. In addition, the combined techniques were used to document 14 cases of terminal transferase-positive acute myeloid leukemia. This study demonstrates that these 2 techniques provide overlapping and complementary information for accurate diagnosis and classification of morphologically difficult hematological malignancies.</description><subject>Acute leukemia</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Surface - analysis</subject><subject>Child</subject><subject>DNA Nucleotidylexotransferase - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>Leukemia - classification</subject><subject>Leukemia - immunology</subject><subject>Leukemia - ultrastructure</subject><subject>leukemia antigens</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Middle Aged</subject><subject>monoclonal antibodies</subject><subject>myeloperoxidase</subject><subject>Peroxidase - metabolism</subject><subject>terminal transferase</subject><subject>ultrastructure</subject><issn>0031-3025</issn><issn>1465-3931</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1OHDEUha0IRBaSB0gRyRXdEHvGM2uLKloFgoREA7Xlse-wBv8s9jgSVDxI8nI8SbzaVZpIyNJ1cc65uudD6AslZx0l4hshHe1Iywci6uNd_wEtKBv6phMdPUCLrd5UQ_8RHef8QAhhnPMjdNQRyljbL1BYrVVSeoZkX2y4x2-vv42dJquLm99e_2ClywzYQXkEb1U-wwrr6EcbwGBwoOcUA_ZWp5h13FiNVTDYel9CdPHeauWwV-kREs5zMc-f0OGkXIbP-_8E3V38uF39bK5vLq9W368bzQiZGy5G1g60hWHSrNYaWiq0qrczM5qWLPtaqc5WCd7RXte5NBMfheqnAVhlcoJOd3s3KT4VyLP0NmtwTgWIJcvlIKhgLa9GujNuG-QEk9wkWy9-lpTILWP5H-Oa-bpfXkYP5l9iD7Xq5zvdhikmr9ag3LzWKoF8iCWFWvzd7fs0VD6_LCSZtYWgwdhUeUsT7Tvpv2SGngM</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Hewson, John W.</creator><creator>Bradstock, K.F.</creator><creator>Kerr, A.</creator><creator>Rose, R.G.</creator><general>Elsevier B.V</general><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1986</creationdate><title>Characterizing “difficult” acute leukemias. a combined electron microscopic and immunological marker study</title><author>Hewson, John W. ; Bradstock, K.F. ; Kerr, A. ; Rose, R.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-89b42612e6fc48356219ca4884dbd20750250752a98315c9837df8b9a5f6e4313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Acute leukemia</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Surface - analysis</topic><topic>Child</topic><topic>DNA Nucleotidylexotransferase - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Infant</topic><topic>Leukemia - classification</topic><topic>Leukemia - immunology</topic><topic>Leukemia - ultrastructure</topic><topic>leukemia antigens</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Middle Aged</topic><topic>monoclonal antibodies</topic><topic>myeloperoxidase</topic><topic>Peroxidase - metabolism</topic><topic>terminal transferase</topic><topic>ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hewson, John W.</creatorcontrib><creatorcontrib>Bradstock, K.F.</creatorcontrib><creatorcontrib>Kerr, A.</creatorcontrib><creatorcontrib>Rose, R.G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hewson, John W.</au><au>Bradstock, K.F.</au><au>Kerr, A.</au><au>Rose, R.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterizing “difficult” acute leukemias. a combined electron microscopic and immunological marker study</atitle><jtitle>Pathology</jtitle><addtitle>Pathology</addtitle><date>1986</date><risdate>1986</risdate><volume>18</volume><issue>1</issue><spage>99</spage><epage>110</epage><pages>99-110</pages><issn>0031-3025</issn><eissn>1465-3931</eissn><abstract>The techniques of transmission electron microscopy (TEM), including ultrastructural myeloperoxidase cytochemistry (MPO), and immunological marker analysis, have been used to classify 58 “difficult” cases of acute leukemia where a precise diagnosis could not be made on the basis of conventional light microscopy and cytochemistry. TEM with MPO proved most valuable in characterizing 15 cases of acute myeloid leukemia and its variants, as well as defining complex cellular subpopulations in 11 cases of chronic myeloid leukemia in blast crisis. Immunological marker studies provided conclusive evidence of lymphoid differentiation in 18 cases of acute lymphoblastic leukemia and related disorders. In addition, the combined techniques were used to document 14 cases of terminal transferase-positive acute myeloid leukemia. This study demonstrates that these 2 techniques provide overlapping and complementary information for accurate diagnosis and classification of morphologically difficult hematological malignancies.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>3014425</pmid><doi>10.3109/00313028609090835</doi><tpages>12</tpages></addata></record> |
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| ispartof | Pathology, 1986, Vol.18 (1), p.99-110 |
| issn | 0031-3025 1465-3931 |
| language | eng |
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| source | MEDLINE; Taylor & Francis Journals Complete; Alma/SFX Local Collection |
| subjects | Acute leukemia Adolescent Adult Aged Antigens, Neoplasm - analysis Antigens, Surface - analysis Child DNA Nucleotidylexotransferase - metabolism Female Humans Infant Leukemia - classification Leukemia - immunology Leukemia - ultrastructure leukemia antigens Male Microscopy, Electron Middle Aged monoclonal antibodies myeloperoxidase Peroxidase - metabolism terminal transferase ultrastructure |
| title | Characterizing “difficult” acute leukemias. a combined electron microscopic and immunological marker study |
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