Isolation and Characterization of 21 Novel Expressed DNA Sequences from the Distal Region of Human Chromosome 4p

We have established an approach to the isolation of expressed DNA sequences from a defined region of the human chromosome. The method relies on the direct screening of cDNA libraries using pooled single-copy microclones generated by a laser chromosome microdissection in conjunction with a single uni...

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Veröffentlicht in:	Genomics 1994-07, Vol.22 (2), p.302-312
Hauptverfasser:	Ishida, Yoshikazu, Hadano, Shinji, Nagayama, Tomiko, Tomiyasu, Hitoshi, Wakasa, Kyo, Ikeda, Joh-E
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences BIOLOGY AND MEDICINE, BASIC STUDIES Chromosome Mapping Chromosomes, Human, Pair 4 Classical genetics, quantitative genetics, hybrids Cloning, Molecular DNA HYBRIDIZATION DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Gene Expression GENETIC MAPPING Genetics of eukaryotes. Biological and molecular evolution Human HUMAN CHROMOSOMES Humans Molecular Sequence Data OPTIMIZATION Polymerase Chain Reaction RNA, Messenger - genetics Sequence Tagged Sites
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