Cyclic AMP formation in Tetrahymena pyriformis is controlled by a K +-conductance
Responses of the cAMP generating system of Tetrahymena to changes in the concentrations of external [K +] or [Ca +] ions were examined. When Tetrahymena are equilibrated in high [K +] buffers, intracellular levels of cAMP decreased to 40% within 2 h. Hyperpolarization of the cells by dilution of ext...
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| Veröffentlicht in: | FEBS letters 1994-12, Vol.356 (2), p.322-326 |
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| creator | Schultz, Joachim E. Schönborn, Christof |
| description | Responses of the cAMP generating system of
Tetrahymena to changes in the concentrations of external [K
+] or [Ca
+] ions were examined. When
Tetrahymena are equilibrated in high [K
+] buffers, intracellular levels of cAMP decreased to 40% within 2 h. Hyperpolarization of the cells by dilution of external [K
+] to one-eighth of its original concentration instantly stimulated intracellular cAMP formation. Manipulations of the K
+ resting conductance of
Tetrahymena by equilibration in buffers of different K
+ content greatly affected the responsivity of the adenylyl cyclase. Hyperpolarization of the cell by addition of Ca
2+ also resulted in a rapid generation of cAMP. Blockade of K
+ conductances by the K
+ channel blockers tetraethylammoniwn, quinine, and Cs
+, dose-dependently inhibited hyperpolarization-stimulated cAMP formation. The data indicate that a hyperpolarization-activated K
+ current is directly coupled to adenylyl cyclase regulation. |
| doi_str_mv | 10.1016/0014-5793(94)01300-4 |
| format | Article |
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Tetrahymena to changes in the concentrations of external [K
+] or [Ca
+] ions were examined. When
Tetrahymena are equilibrated in high [K
+] buffers, intracellular levels of cAMP decreased to 40% within 2 h. Hyperpolarization of the cells by dilution of external [K
+] to one-eighth of its original concentration instantly stimulated intracellular cAMP formation. Manipulations of the K
+ resting conductance of
Tetrahymena by equilibration in buffers of different K
+ content greatly affected the responsivity of the adenylyl cyclase. Hyperpolarization of the cell by addition of Ca
2+ also resulted in a rapid generation of cAMP. Blockade of K
+ conductances by the K
+ channel blockers tetraethylammoniwn, quinine, and Cs
+, dose-dependently inhibited hyperpolarization-stimulated cAMP formation. The data indicate that a hyperpolarization-activated K
+ current is directly coupled to adenylyl cyclase regulation.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(94)01300-4</identifier><identifier>PMID: 7805864</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Calcium - pharmacology ; cAMP ; Cesium - pharmacology ; Cyclic AMP - metabolism ; Hyperpolarization ; K + channel ; Kinetics ; Potassium - pharmacology ; Potassium Channel Blockers ; Quinine - pharmacology ; Tetraethylammonium ; Tetraethylammonium Compounds - pharmacology ; Tetrahymena ; Tetrahymena pyriformis - drug effects ; Tetrahymena pyriformis - physiology ; Time Factors</subject><ispartof>FEBS letters, 1994-12, Vol.356 (2), p.322-326</ispartof><rights>1994</rights><rights>FEBS Letters 356 (1994) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4204-f125c2d847619defc14ef63a9c5bf6f8b0c7869f9cba88de1121bbb2f16186f13</citedby><cites>FETCH-LOGICAL-c4204-f125c2d847619defc14ef63a9c5bf6f8b0c7869f9cba88de1121bbb2f16186f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579394013004$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7805864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schultz, Joachim E.</creatorcontrib><creatorcontrib>Schönborn, Christof</creatorcontrib><title>Cyclic AMP formation in Tetrahymena pyriformis is controlled by a K +-conductance</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Responses of the cAMP generating system of
Tetrahymena to changes in the concentrations of external [K
+] or [Ca
+] ions were examined. When
Tetrahymena are equilibrated in high [K
+] buffers, intracellular levels of cAMP decreased to 40% within 2 h. Hyperpolarization of the cells by dilution of external [K
+] to one-eighth of its original concentration instantly stimulated intracellular cAMP formation. Manipulations of the K
+ resting conductance of
Tetrahymena by equilibration in buffers of different K
+ content greatly affected the responsivity of the adenylyl cyclase. Hyperpolarization of the cell by addition of Ca
2+ also resulted in a rapid generation of cAMP. Blockade of K
+ conductances by the K
+ channel blockers tetraethylammoniwn, quinine, and Cs
+, dose-dependently inhibited hyperpolarization-stimulated cAMP formation. The data indicate that a hyperpolarization-activated K
+ current is directly coupled to adenylyl cyclase regulation.</description><subject>Animals</subject><subject>Calcium - pharmacology</subject><subject>cAMP</subject><subject>Cesium - pharmacology</subject><subject>Cyclic AMP - metabolism</subject><subject>Hyperpolarization</subject><subject>K + channel</subject><subject>Kinetics</subject><subject>Potassium - pharmacology</subject><subject>Potassium Channel Blockers</subject><subject>Quinine - pharmacology</subject><subject>Tetraethylammonium</subject><subject>Tetraethylammonium Compounds - pharmacology</subject><subject>Tetrahymena</subject><subject>Tetrahymena pyriformis - drug effects</subject><subject>Tetrahymena pyriformis - physiology</subject><subject>Time Factors</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFFr2zAUhUVZydKu_2ADPY2W4k3XVmTpZZCGpi3t2Arts5DlK6bh2JnktPjfV05CHstAcNE55x4uHyGfgX0DBuI7Y8CzWamKc8UvGBSMZfyITEGWRVZwIT-Q6SHykZzE-JelvwQ1IZNSspkUfEoeF4NtvKXzn7-p68LK9L5rqW_pE_bB_BlW2Bq6HoIfTR9perZr-9A1Dda0Gqih9_QyS1q9sb1pLX4ix840Ec_285Q8L6-fFrfZw6-bu8X8IbM8ZzxzkM9sXkteClA1OgscnSiMsrPKCScrZksplFO2MlLWCJBDVVW5AwFSOChOyddd7zp0_zYYe53us9g0psVuE3UpFBMqL1KQ74I2dDEGdHod_MqEQQPTI0k9YtIjJq243pLUPK192fdvqhXWh6U9uuQvd_6rb3D4r069vL7KR2PUFd-qY9GPXREmWi8eg47WYyJZ-4C213Xn37_0DUVrlRU</recordid><startdate>19941219</startdate><enddate>19941219</enddate><creator>Schultz, Joachim E.</creator><creator>Schönborn, Christof</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941219</creationdate><title>Cyclic AMP formation in Tetrahymena pyriformis is controlled by a K +-conductance</title><author>Schultz, Joachim E. ; Schönborn, Christof</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4204-f125c2d847619defc14ef63a9c5bf6f8b0c7869f9cba88de1121bbb2f16186f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Calcium - pharmacology</topic><topic>cAMP</topic><topic>Cesium - pharmacology</topic><topic>Cyclic AMP - metabolism</topic><topic>Hyperpolarization</topic><topic>K + channel</topic><topic>Kinetics</topic><topic>Potassium - pharmacology</topic><topic>Potassium Channel Blockers</topic><topic>Quinine - pharmacology</topic><topic>Tetraethylammonium</topic><topic>Tetraethylammonium Compounds - pharmacology</topic><topic>Tetrahymena</topic><topic>Tetrahymena pyriformis - drug effects</topic><topic>Tetrahymena pyriformis - physiology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schultz, Joachim E.</creatorcontrib><creatorcontrib>Schönborn, Christof</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schultz, Joachim E.</au><au>Schönborn, Christof</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cyclic AMP formation in Tetrahymena pyriformis is controlled by a K +-conductance</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1994-12-19</date><risdate>1994</risdate><volume>356</volume><issue>2</issue><spage>322</spage><epage>326</epage><pages>322-326</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Responses of the cAMP generating system of
Tetrahymena to changes in the concentrations of external [K
+] or [Ca
+] ions were examined. When
Tetrahymena are equilibrated in high [K
+] buffers, intracellular levels of cAMP decreased to 40% within 2 h. Hyperpolarization of the cells by dilution of external [K
+] to one-eighth of its original concentration instantly stimulated intracellular cAMP formation. Manipulations of the K
+ resting conductance of
Tetrahymena by equilibration in buffers of different K
+ content greatly affected the responsivity of the adenylyl cyclase. Hyperpolarization of the cell by addition of Ca
2+ also resulted in a rapid generation of cAMP. Blockade of K
+ conductances by the K
+ channel blockers tetraethylammoniwn, quinine, and Cs
+, dose-dependently inhibited hyperpolarization-stimulated cAMP formation. The data indicate that a hyperpolarization-activated K
+ current is directly coupled to adenylyl cyclase regulation.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>7805864</pmid><doi>10.1016/0014-5793(94)01300-4</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1994-12, Vol.356 (2), p.322-326 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76906923 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Calcium - pharmacology cAMP Cesium - pharmacology Cyclic AMP - metabolism Hyperpolarization K + channel Kinetics Potassium - pharmacology Potassium Channel Blockers Quinine - pharmacology Tetraethylammonium Tetraethylammonium Compounds - pharmacology Tetrahymena Tetrahymena pyriformis - drug effects Tetrahymena pyriformis - physiology Time Factors |
| title | Cyclic AMP formation in Tetrahymena pyriformis is controlled by a K +-conductance |
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