Group-selective immunoassay
A new solid-phase immunoassay technique has been applied for anti-digoxin monoclonal antibodies (Mabs) detection. At the first assay step, anti-digoxin Mabs (IgG1, K aff = 9.2 × 10 9 M −1) were bound to a specially prepared immunosorbent, the microtiter plates coated with digoxin-human serum albumin...
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| Veröffentlicht in: | Immunology letters 1994-07, Vol.41 (2), p.235-239 |
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| container_title | Immunology letters |
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| creator | Yazynin, Sergey A. Deyev, Sergey M. |
| description | A new solid-phase immunoassay technique has been applied for anti-digoxin monoclonal antibodies (Mabs) detection. At the first assay step, anti-digoxin Mabs (IgG1, K
aff = 9.2 × 10
9 M
−1) were bound to a specially prepared immunosorbent, the microtiter plates coated with digoxin-human serum albumin conjugate (Dig-HSA), in which free amino groups were protected by a glutaraldehyde cross-linking modification. The modification did not essentially influence the antibody-binding capacity of the immunosorbent. After antigen-antibody reaction, free amino groups were located only on the anti-digoxin Mabs, bound to chemically modified immunosorbent. At the second assay step, free amino groups of anti-digoxin Mabs were biotinylated by
N-hydroxysuccinimide-biotin ester. Then the biotin residues were detected by the streptavidin-peroxidase conjugate. The method does not require second labeled antibodies and may be used for anti-hapten hybridoma screening. |
| doi_str_mv | 10.1016/0165-2478(94)90139-2 |
| format | Article |
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aff = 9.2 × 10
9 M
−1) were bound to a specially prepared immunosorbent, the microtiter plates coated with digoxin-human serum albumin conjugate (Dig-HSA), in which free amino groups were protected by a glutaraldehyde cross-linking modification. The modification did not essentially influence the antibody-binding capacity of the immunosorbent. After antigen-antibody reaction, free amino groups were located only on the anti-digoxin Mabs, bound to chemically modified immunosorbent. At the second assay step, free amino groups of anti-digoxin Mabs were biotinylated by
N-hydroxysuccinimide-biotin ester. Then the biotin residues were detected by the streptavidin-peroxidase conjugate. The method does not require second labeled antibodies and may be used for anti-hapten hybridoma screening.</description><identifier>ISSN: 0165-2478</identifier><identifier>EISSN: 1879-0542</identifier><identifier>DOI: 10.1016/0165-2478(94)90139-2</identifier><identifier>PMID: 8002044</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antibodies, Monoclonal - analysis ; Digoxin ; Digoxin - immunology ; Enzyme-Linked Immunosorbent Assay - methods ; Group-selective immunoassay ; Humans ; Immunoassay - methods ; Immunoenzyme Techniques ; Monoclonal antibody ; Streptavidin-biotin system</subject><ispartof>Immunology letters, 1994-07, Vol.41 (2), p.235-239</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-a016d2ab729ae6b4389175dca581d10612f3763acc79ad8ff3f6e3a638d3a66a3</citedby><cites>FETCH-LOGICAL-c388t-a016d2ab729ae6b4389175dca581d10612f3763acc79ad8ff3f6e3a638d3a66a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0165247894901392$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8002044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yazynin, Sergey A.</creatorcontrib><creatorcontrib>Deyev, Sergey M.</creatorcontrib><title>Group-selective immunoassay</title><title>Immunology letters</title><addtitle>Immunol Lett</addtitle><description>A new solid-phase immunoassay technique has been applied for anti-digoxin monoclonal antibodies (Mabs) detection. At the first assay step, anti-digoxin Mabs (IgG1, K
aff = 9.2 × 10
9 M
−1) were bound to a specially prepared immunosorbent, the microtiter plates coated with digoxin-human serum albumin conjugate (Dig-HSA), in which free amino groups were protected by a glutaraldehyde cross-linking modification. The modification did not essentially influence the antibody-binding capacity of the immunosorbent. After antigen-antibody reaction, free amino groups were located only on the anti-digoxin Mabs, bound to chemically modified immunosorbent. At the second assay step, free amino groups of anti-digoxin Mabs were biotinylated by
N-hydroxysuccinimide-biotin ester. Then the biotin residues were detected by the streptavidin-peroxidase conjugate. The method does not require second labeled antibodies and may be used for anti-hapten hybridoma screening.</description><subject>Antibodies, Monoclonal - analysis</subject><subject>Digoxin</subject><subject>Digoxin - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Group-selective immunoassay</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Immunoenzyme Techniques</subject><subject>Monoclonal antibody</subject><subject>Streptavidin-biotin system</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEUhYMotVZ_gQquRBfRvCaPjSBFq1Bwo-uQJncgMtOpSUfovzdjS5e6uPcuzrnnwIfQBSV3lFB5X6bCTCh9Y8StIZQbzA7QmGplMKkEO0TjveUYneT8SQituOAjNNKEMCLEGJ3PUtevcIYG_Dp-w1Vs237ZuZzd5hQd1a7JcLa7E_Tx_PQ-fcHzt9nr9HGOPdd6jV1pCcwtFDMO5EJwbaiqgneVpoESSVnNleTOe2Vc0HXNawncSa5D2dLxCbre5q5S99VDXts2Zg9N45bQ9dkqaUhVQv81Uik1oYQVo9gafepyTlDbVYqtSxtLiR3g2YGMHchYI-wvPDu8Xe7y-0ULYf-0o1X0h60OhcZ3hGSzj7D0EGIq-Gzo4t8FP0sJfDQ</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>Yazynin, Sergey A.</creator><creator>Deyev, Sergey M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940701</creationdate><title>Group-selective immunoassay</title><author>Yazynin, Sergey A. ; Deyev, Sergey M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-a016d2ab729ae6b4389175dca581d10612f3763acc79ad8ff3f6e3a638d3a66a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antibodies, Monoclonal - analysis</topic><topic>Digoxin</topic><topic>Digoxin - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Group-selective immunoassay</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Immunoenzyme Techniques</topic><topic>Monoclonal antibody</topic><topic>Streptavidin-biotin system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yazynin, Sergey A.</creatorcontrib><creatorcontrib>Deyev, Sergey M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yazynin, Sergey A.</au><au>Deyev, Sergey M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Group-selective immunoassay</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>1994-07-01</date><risdate>1994</risdate><volume>41</volume><issue>2</issue><spage>235</spage><epage>239</epage><pages>235-239</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><abstract>A new solid-phase immunoassay technique has been applied for anti-digoxin monoclonal antibodies (Mabs) detection. At the first assay step, anti-digoxin Mabs (IgG1, K
aff = 9.2 × 10
9 M
−1) were bound to a specially prepared immunosorbent, the microtiter plates coated with digoxin-human serum albumin conjugate (Dig-HSA), in which free amino groups were protected by a glutaraldehyde cross-linking modification. The modification did not essentially influence the antibody-binding capacity of the immunosorbent. After antigen-antibody reaction, free amino groups were located only on the anti-digoxin Mabs, bound to chemically modified immunosorbent. At the second assay step, free amino groups of anti-digoxin Mabs were biotinylated by
N-hydroxysuccinimide-biotin ester. Then the biotin residues were detected by the streptavidin-peroxidase conjugate. The method does not require second labeled antibodies and may be used for anti-hapten hybridoma screening.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8002044</pmid><doi>10.1016/0165-2478(94)90139-2</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2478 |
| ispartof | Immunology letters, 1994-07, Vol.41 (2), p.235-239 |
| issn | 0165-2478 1879-0542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76905891 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antibodies, Monoclonal - analysis Digoxin Digoxin - immunology Enzyme-Linked Immunosorbent Assay - methods Group-selective immunoassay Humans Immunoassay - methods Immunoenzyme Techniques Monoclonal antibody Streptavidin-biotin system |
| title | Group-selective immunoassay |
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