Estimation of vitamin D3 and 25-hydroxyvitamin D3 in muscle and adipose tissue of rats and man
An assay is described based on a high pressure liquid chromatography system for measurement of vitamin D3 and 25-hydroxyvitamin D3 concentration in adipose tissue and muscle. The sensitivity of the assay is less than 1 ng/g of tissue. Neither vitamin D3 nor 25-hydroxyvitamin D3 were found in tissues...
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| Veröffentlicht in: | Clinica chimica acta 1986-06, Vol.157 (2), p.175-181 |
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| container_title | Clinica chimica acta |
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| creator | LAWSON, D. E. M DOUGLAS, J LEAN, M SEDRANI, S |
| description | An assay is described based on a high pressure liquid chromatography system for measurement of vitamin D3 and 25-hydroxyvitamin D3 concentration in adipose tissue and muscle. The sensitivity of the assay is less than 1 ng/g of tissue. Neither vitamin D3 nor 25-hydroxyvitamin D3 were found in tissues of vitamin D-deficient rats using this method. 25-Hydroxyvitamin D3 could not be detected in adipose tissue and very little was found in muscle of normal rats. The mean vitamin D3 concentrations in 15 samples of human perirenal adipose tissue was 45.3 ng/g +/- 22.2 (SD) and in 6 samples of axillary tissue 115.6 ng/g +/- 52.4 (SD). Human adipose tissue contains a substantial amount of vitamin D3 and its contribution to the maintenance of vitamin D status is discussed. |
| doi_str_mv | 10.1016/0009-8981(86)90223-8 |
| format | Article |
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Human adipose tissue contains a substantial amount of vitamin D3 and its contribution to the maintenance of vitamin D status is discussed.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(86)90223-8</identifier><identifier>PMID: 3013467</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier</publisher><subject>Adipose Tissue - analysis ; Adolescent ; Adult ; Aged ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Calcifediol - analysis ; Child ; Cholecalciferol - analysis ; Chromatography, High Pressure Liquid ; Coenzymes, vitamins ; Female ; Fundamental and applied biological sciences. 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E. M</creatorcontrib><creatorcontrib>DOUGLAS, J</creatorcontrib><creatorcontrib>LEAN, M</creatorcontrib><creatorcontrib>SEDRANI, S</creatorcontrib><title>Estimation of vitamin D3 and 25-hydroxyvitamin D3 in muscle and adipose tissue of rats and man</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>An assay is described based on a high pressure liquid chromatography system for measurement of vitamin D3 and 25-hydroxyvitamin D3 concentration in adipose tissue and muscle. The sensitivity of the assay is less than 1 ng/g of tissue. Neither vitamin D3 nor 25-hydroxyvitamin D3 were found in tissues of vitamin D-deficient rats using this method. 25-Hydroxyvitamin D3 could not be detected in adipose tissue and very little was found in muscle of normal rats. The mean vitamin D3 concentrations in 15 samples of human perirenal adipose tissue was 45.3 ng/g +/- 22.2 (SD) and in 6 samples of axillary tissue 115.6 ng/g +/- 52.4 (SD). Human adipose tissue contains a substantial amount of vitamin D3 and its contribution to the maintenance of vitamin D status is discussed.</description><subject>Adipose Tissue - analysis</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcifediol - analysis</subject><subject>Child</subject><subject>Cholecalciferol - analysis</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Coenzymes, vitamins</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Muscles - analysis</subject><subject>Other biological molecules</subject><subject>Rats</subject><subject>Vitamin D Deficiency - metabolism</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkFtLwzAYhoMoc07_gUIvRPSimtOa5FLmPMDAG701pEmKkR5mv1bcvzfdyvAiCeF9vpfkQeic4FuCSXaHMVapVJJcy-xGYUpZKg_QlEjBUsYVPUTTPXKMTgC-4pXjjEzQhGHCeCam6GMJXahMF5o6aYrkJ3SmCnXywBJTu4TO08-Na5vfzb8g7lUPtvRbxLiwbsAnXQDo_dDRmg62UWXqU3RUmBL82XjO0Pvj8m3xnK5en14W96vUMka6NHeMey-IMyKjkscHz0meW0Jzzh0W1FoplXIE27jmuaFUSEykw55ZkkvDZuhq17tum-_eQ6erANaXpal904MWmVRCYR5BvgNt2wC0vtDrNv6_3WiC9aBVD8704EzLTG-1ahnHLsb-Pq-82w-NHmN-OeYGrCmL1tQ2wB6TYiiS7A89bn5Q</recordid><startdate>19860615</startdate><enddate>19860615</enddate><creator>LAWSON, D. E. 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Psychology</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Muscles - analysis</topic><topic>Other biological molecules</topic><topic>Rats</topic><topic>Vitamin D Deficiency - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LAWSON, D. E. M</creatorcontrib><creatorcontrib>DOUGLAS, J</creatorcontrib><creatorcontrib>LEAN, M</creatorcontrib><creatorcontrib>SEDRANI, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LAWSON, D. E. 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The mean vitamin D3 concentrations in 15 samples of human perirenal adipose tissue was 45.3 ng/g +/- 22.2 (SD) and in 6 samples of axillary tissue 115.6 ng/g +/- 52.4 (SD). Human adipose tissue contains a substantial amount of vitamin D3 and its contribution to the maintenance of vitamin D status is discussed.</abstract><cop>Shannon</cop><pub>Elsevier</pub><pmid>3013467</pmid><doi>10.1016/0009-8981(86)90223-8</doi><tpages>7</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1986-06, Vol.157 (2), p.175-181 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adipose Tissue - analysis Adolescent Adult Aged Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Calcifediol - analysis Child Cholecalciferol - analysis Chromatography, High Pressure Liquid Coenzymes, vitamins Female Fundamental and applied biological sciences. Psychology Humans Male Middle Aged Muscles - analysis Other biological molecules Rats Vitamin D Deficiency - metabolism |
| title | Estimation of vitamin D3 and 25-hydroxyvitamin D3 in muscle and adipose tissue of rats and man |
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