Biophysical and pharmacological properties of large conductance Ca(2+)-activated K+ channels in N1E-115 cells
The large conductance Ca(2+)-activated K+ channels in differentiated mouse neuroblastoma N1E-115 cells have been studied using patch-clamp single-channel current recording in excised membrane patches. These channels displayed a unitary conductance of 200 pS under symmetrical K+ concentrations. Effec...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-11, Vol.205 (1), p.596-602 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Diserbo, M Antonny, B Verdetti, J |
| description | The large conductance Ca(2+)-activated K+ channels in differentiated mouse neuroblastoma N1E-115 cells have been studied using patch-clamp single-channel current recording in excised membrane patches. These channels displayed a unitary conductance of 200 pS under symmetrical K+ concentrations. Effects of blockade by TEA+, Cs+ and Ba2+ were different and argued for distinct action mechanisms. The open probability of these channels increased with increasing internal calcium and membrane potential. Maximum sensitivity of these channels ranged over physiological variations of internal calcium at membrane potentials close to zero, suggesting a physiological role for these channels in regulating the membrane potential and Ca2+ influx through voltage-dependent Ca2+ channels. |
| doi_str_mv | 10.1006/bbrc.1994.2707 |
| format | Article |
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Maximum sensitivity of these channels ranged over physiological variations of internal calcium at membrane potentials close to zero, suggesting a physiological role for these channels in regulating the membrane potential and Ca2+ influx through voltage-dependent Ca2+ channels.</description><subject>Animals</subject><subject>Barium - pharmacology</subject><subject>Calcium - physiology</subject><subject>Cesium - pharmacology</subject><subject>Electrophysiology</subject><subject>Ion Channel Gating</subject><subject>Mice</subject><subject>Neuroblastoma - pathology</subject><subject>Potassium Channel Blockers</subject><subject>Potassium Channels - physiology</subject><subject>Tetraethylammonium</subject><subject>Tetraethylammonium Compounds - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0006-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkM1Lw0AUxPeg1Fq9ehP2JIqk7tt8bPaopX5g0YuCt_Dy9qWN5MtsIvS_t2phYGD4MQwjxBmoOSiV3OR5T3OwNppro8yBmKpdGmgLH0fi2PtPpQCixE7ExFhrVRpPRX1Xtt1m60vCSmLjZLfBvkZqq3b9l3V923E_lOxlW8gK-zVLahs30oANsVzgpb6-CpCG8hsHdvL5WtIGm4YrL8tGvsAyAIglcVX5E3FYYOX5dO8z8X6_fFs8BqvXh6fF7SroQMMQJC5J4sIlOYeKWIUOmGJNVtvYWWMIqXA6RMtFDHkKUWFMAahZx0zOpBzOxMV_727918h-yOrS_y7AhtvRZyZJbbTTDjzfg2Nes8u6vqyx32b7f8IfXJBmLg</recordid><startdate>19941130</startdate><enddate>19941130</enddate><creator>Diserbo, M</creator><creator>Antonny, B</creator><creator>Verdetti, J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19941130</creationdate><title>Biophysical and pharmacological properties of large conductance Ca(2+)-activated K+ channels in N1E-115 cells</title><author>Diserbo, M ; Antonny, B ; Verdetti, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p121t-6d665fd6be30ce03d1ec52c9295d977cacfd23a9ef51b814f77f1a2e25ecd78e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Barium - pharmacology</topic><topic>Calcium - physiology</topic><topic>Cesium - pharmacology</topic><topic>Electrophysiology</topic><topic>Ion Channel Gating</topic><topic>Mice</topic><topic>Neuroblastoma - pathology</topic><topic>Potassium Channel Blockers</topic><topic>Potassium Channels - physiology</topic><topic>Tetraethylammonium</topic><topic>Tetraethylammonium Compounds - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diserbo, M</creatorcontrib><creatorcontrib>Antonny, B</creatorcontrib><creatorcontrib>Verdetti, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diserbo, M</au><au>Antonny, B</au><au>Verdetti, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biophysical and pharmacological properties of large conductance Ca(2+)-activated K+ channels in N1E-115 cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-11-30</date><risdate>1994</risdate><volume>205</volume><issue>1</issue><spage>596</spage><epage>602</epage><pages>596-602</pages><issn>0006-291X</issn><abstract>The large conductance Ca(2+)-activated K+ channels in differentiated mouse neuroblastoma N1E-115 cells have been studied using patch-clamp single-channel current recording in excised membrane patches. 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| ispartof | Biochemical and biophysical research communications, 1994-11, Vol.205 (1), p.596-602 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Barium - pharmacology Calcium - physiology Cesium - pharmacology Electrophysiology Ion Channel Gating Mice Neuroblastoma - pathology Potassium Channel Blockers Potassium Channels - physiology Tetraethylammonium Tetraethylammonium Compounds - pharmacology Tumor Cells, Cultured |
| title | Biophysical and pharmacological properties of large conductance Ca(2+)-activated K+ channels in N1E-115 cells |
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