Mutations downstream of the polyadenylation site of a Xenopus β-globin mRNA affect the position but not the efficiency of 3′ processing
In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis β1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacemen...
Gespeichert in:
| Veröffentlicht in: | Cell 1986-07, Vol.46 (2), p.263-270 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 270 |
|---|---|
| container_issue | 2 |
| container_start_page | 263 |
| container_title | Cell |
| container_volume | 46 |
| creator | Mason, Philip J. Elkington, Jane A. Lloyd, Malgorzata M. Jones, Michael B. Williams, Jeffrey G. |
| description | In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis β1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacement mutations, which lie in the region from 8 to 39 bp downstream of the AATAAA sequence and which effectively remove previously identified second components of the polyadenylation signal, do not greatly reduce the efficiency of processing, but in some cases alter the precise site of cleavage. We conclude that sequences downstream of the polyadenylation site affect the position of 3′ RNA processing, but have minimal effects on its efficiency. |
| doi_str_mv | 10.1016/0092-8674(86)90743-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76891816</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0092867486907439</els_id><sourcerecordid>76891816</sourcerecordid><originalsourceid>FETCH-LOGICAL-c332t-172b558ced4528187cf65c543e0cd65a45bdd4a7c0abeddd2afe36408989f46f3</originalsourceid><addsrcrecordid>eNqFkc-K1TAYxYMo453RN1DIQmRcVJM2fzfCMPgPRgVRcBfS5MsYaZNr0yp359rHmQfxIXwS23vLXeomgZzfOUm-g9ADSp5SQsUzQnRdKSHZuRJPNJGsqfQttKFEy4pRWd9GmyNyF52W8pUQojjnJ-ikVrLWkmzQr7fTaMeYU8E-_0hlHMD2OAc8fgG8zd3Oeki7bo_gEkdYNIs_Q8rbqeDfN9V1l9uYcP_h3QW2IYAbV-9ML6Z2GnHKh0MIIboIye2WmObPzxu8HbKDUmK6vofuBNsVuL_uZ-jTyxcfL19XV-9fvbm8uKpc09RjNf-s5Vw58IzXiirpguCOswaI84JbxlvvmZWO2Ba897UN0AhGlFY6MBGaM_T4kDtf_W2CMpo-FgddZxPkqRgplKaKiv-ClDFRayVnkB1AN-RSBghmO8TeDjtDiVm6MksRZiliXsy-K6Nn28M1f2p78EfTWs6sP1p1W5ztwmCTi-WIKVoT1dAZe37AYB7a9wiDKfsZg4_D3IbxOf77HX8BWbizxA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14462987</pqid></control><display><type>article</type><title>Mutations downstream of the polyadenylation site of a Xenopus β-globin mRNA affect the position but not the efficiency of 3′ processing</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Mason, Philip J. ; Elkington, Jane A. ; Lloyd, Malgorzata M. ; Jones, Michael B. ; Williams, Jeffrey G.</creator><creatorcontrib>Mason, Philip J. ; Elkington, Jane A. ; Lloyd, Malgorzata M. ; Jones, Michael B. ; Williams, Jeffrey G.</creatorcontrib><description>In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis β1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacement mutations, which lie in the region from 8 to 39 bp downstream of the AATAAA sequence and which effectively remove previously identified second components of the polyadenylation signal, do not greatly reduce the efficiency of processing, but in some cases alter the precise site of cleavage. We conclude that sequences downstream of the polyadenylation site affect the position of 3′ RNA processing, but have minimal effects on its efficiency.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(86)90743-9</identifier><identifier>PMID: 2872970</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; DNA - genetics ; Fundamental and applied biological sciences. Psychology ; Globins - genetics ; Molecular and cellular biology ; Molecular genetics ; Mutation ; Nucleic Acid Hybridization ; Poly A - metabolism ; RNA Processing, Post-Transcriptional ; RNA, Messenger - metabolism ; Transcription. Transcription factor. Splicing. Rna processing ; Xenopus laevis</subject><ispartof>Cell, 1986-07, Vol.46 (2), p.263-270</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-172b558ced4528187cf65c543e0cd65a45bdd4a7c0abeddd2afe36408989f46f3</citedby><cites>FETCH-LOGICAL-c332t-172b558ced4528187cf65c543e0cd65a45bdd4a7c0abeddd2afe36408989f46f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(86)90743-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8120831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2872970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mason, Philip J.</creatorcontrib><creatorcontrib>Elkington, Jane A.</creatorcontrib><creatorcontrib>Lloyd, Malgorzata M.</creatorcontrib><creatorcontrib>Jones, Michael B.</creatorcontrib><creatorcontrib>Williams, Jeffrey G.</creatorcontrib><title>Mutations downstream of the polyadenylation site of a Xenopus β-globin mRNA affect the position but not the efficiency of 3′ processing</title><title>Cell</title><addtitle>Cell</addtitle><description>In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis β1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacement mutations, which lie in the region from 8 to 39 bp downstream of the AATAAA sequence and which effectively remove previously identified second components of the polyadenylation signal, do not greatly reduce the efficiency of processing, but in some cases alter the precise site of cleavage. We conclude that sequences downstream of the polyadenylation site affect the position of 3′ RNA processing, but have minimal effects on its efficiency.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Globins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Nucleic Acid Hybridization</subject><subject>Poly A - metabolism</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Xenopus laevis</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-K1TAYxYMo453RN1DIQmRcVJM2fzfCMPgPRgVRcBfS5MsYaZNr0yp359rHmQfxIXwS23vLXeomgZzfOUm-g9ADSp5SQsUzQnRdKSHZuRJPNJGsqfQttKFEy4pRWd9GmyNyF52W8pUQojjnJ-ikVrLWkmzQr7fTaMeYU8E-_0hlHMD2OAc8fgG8zd3Oeki7bo_gEkdYNIs_Q8rbqeDfN9V1l9uYcP_h3QW2IYAbV-9ML6Z2GnHKh0MIIboIye2WmObPzxu8HbKDUmK6vofuBNsVuL_uZ-jTyxcfL19XV-9fvbm8uKpc09RjNf-s5Vw58IzXiirpguCOswaI84JbxlvvmZWO2Ba897UN0AhGlFY6MBGaM_T4kDtf_W2CMpo-FgddZxPkqRgplKaKiv-ClDFRayVnkB1AN-RSBghmO8TeDjtDiVm6MksRZiliXsy-K6Nn28M1f2p78EfTWs6sP1p1W5ztwmCTi-WIKVoT1dAZe37AYB7a9wiDKfsZg4_D3IbxOf77HX8BWbizxA</recordid><startdate>19860718</startdate><enddate>19860718</enddate><creator>Mason, Philip J.</creator><creator>Elkington, Jane A.</creator><creator>Lloyd, Malgorzata M.</creator><creator>Jones, Michael B.</creator><creator>Williams, Jeffrey G.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19860718</creationdate><title>Mutations downstream of the polyadenylation site of a Xenopus β-globin mRNA affect the position but not the efficiency of 3′ processing</title><author>Mason, Philip J. ; Elkington, Jane A. ; Lloyd, Malgorzata M. ; Jones, Michael B. ; Williams, Jeffrey G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-172b558ced4528187cf65c543e0cd65a45bdd4a7c0abeddd2afe36408989f46f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Globins - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutation</topic><topic>Nucleic Acid Hybridization</topic><topic>Poly A - metabolism</topic><topic>RNA Processing, Post-Transcriptional</topic><topic>RNA, Messenger - metabolism</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mason, Philip J.</creatorcontrib><creatorcontrib>Elkington, Jane A.</creatorcontrib><creatorcontrib>Lloyd, Malgorzata M.</creatorcontrib><creatorcontrib>Jones, Michael B.</creatorcontrib><creatorcontrib>Williams, Jeffrey G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mason, Philip J.</au><au>Elkington, Jane A.</au><au>Lloyd, Malgorzata M.</au><au>Jones, Michael B.</au><au>Williams, Jeffrey G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutations downstream of the polyadenylation site of a Xenopus β-globin mRNA affect the position but not the efficiency of 3′ processing</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1986-07-18</date><risdate>1986</risdate><volume>46</volume><issue>2</issue><spage>263</spage><epage>270</epage><pages>263-270</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis β1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacement mutations, which lie in the region from 8 to 39 bp downstream of the AATAAA sequence and which effectively remove previously identified second components of the polyadenylation signal, do not greatly reduce the efficiency of processing, but in some cases alter the precise site of cleavage. We conclude that sequences downstream of the polyadenylation site affect the position of 3′ RNA processing, but have minimal effects on its efficiency.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>2872970</pmid><doi>10.1016/0092-8674(86)90743-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 1986-07, Vol.46 (2), p.263-270 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76891816 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Base Sequence Biological and medical sciences DNA - genetics Fundamental and applied biological sciences. Psychology Globins - genetics Molecular and cellular biology Molecular genetics Mutation Nucleic Acid Hybridization Poly A - metabolism RNA Processing, Post-Transcriptional RNA, Messenger - metabolism Transcription. Transcription factor. Splicing. Rna processing Xenopus laevis |
| title | Mutations downstream of the polyadenylation site of a Xenopus β-globin mRNA affect the position but not the efficiency of 3′ processing |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T03%3A34%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mutations%20downstream%20of%20the%20polyadenylation%20site%20of%20a%20Xenopus%20%CE%B2-globin%20mRNA%20affect%20the%20position%20but%20not%20the%20efficiency%20of%203%E2%80%B2%20processing&rft.jtitle=Cell&rft.au=Mason,%20Philip%20J.&rft.date=1986-07-18&rft.volume=46&rft.issue=2&rft.spage=263&rft.epage=270&rft.pages=263-270&rft.issn=0092-8674&rft.eissn=1097-4172&rft.coden=CELLB5&rft_id=info:doi/10.1016/0092-8674(86)90743-9&rft_dat=%3Cproquest_cross%3E76891816%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14462987&rft_id=info:pmid/2872970&rft_els_id=0092867486907439&rfr_iscdi=true |