Identification as β-Adducin of a Protein Interacting with Rabphilin-3A in the Presence of Ca2+ and Phosphatidylserine
Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein implicated in neurotransmitter release. We have previously identified a Rabphilin-3A-interacting protein with a Mr of about 115 kDa in bovine brain. We have attempted here to purify this protein and to determine its primar...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1994-11, Vol.205 (1), p.460-466 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 466 |
|---|---|
| container_issue | 1 |
| container_start_page | 460 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 205 |
| creator | Miyazaki, M. Shirataki, H. Kohno, H. Kaibuchi, K. Tsugita, A. Takai, Y. |
| description | Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein implicated in neurotransmitter release. We have previously identified a Rabphilin-3A-interacting protein with a Mr of about 115 kDa in bovine brain. We have attempted here to purify this protein and to determine its primary structure. Amino acid sequence analysis has revealed that this protein is a bovine counterpart of human β-adducin which is known to be a good substrate for protein kinase C. The Rabphilin-3A-interacting protein also binds to protein kinase C in the presence of Ca2+ and phosphatidylserine. These results indicate that Rabphilin-3A binds to β-adducin in the presence of Ca2+ and phosphatidylserine. |
| doi_str_mv | 10.1006/bbrc.1994.2688 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76891117</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X8472688X</els_id><sourcerecordid>76891117</sourcerecordid><originalsourceid>FETCH-LOGICAL-c271t-70daf0a174977ea867ea5b0306550b3c2385f22e4fc2c9dfc9ad134422a883d23</originalsourceid><addsrcrecordid>eNp1kMtKAzEUhoMotVa37oSs3MjUJDOdmSxL8VIoWETBXcgkZ5xIm6lJptLX8kF8JjO0uHOTC-c7_0k-hC4pGVNC8tuqcmpMOc_GLC_LIzSkhJOEUZIdoyGJRMI4fTtFZ95_EEJplvMBGhScc5JPhmg712CDqY2SwbQWS49_vpOp1p0yFrc1lnjp2gDxMrcBnFTB2Hf8ZUKDn2W1aczK2CSd4giEBiIMHqyCvnUm2Q2WVuNl0_pNEwfo3cqDMxbO0Ukt4_nisI_Q6_3dy-wxWTw9zGfTRaJYQUNSEC1rImmR8aIAWeZxmVQkjU-fkCpVLC0nNWOQ1YoprmvFpaZpljEmyzLVLB2h633uxrWfHfgg1sYrWK2khbbzoshLTiktIjjeg8q13juoxcaZtXQ7QYnoRYtetOhFi150bLg6JHfVGvQffjAb6-W-DvF7WwNOeGV6M9o4UEHo1vwX_QtENY2C</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76891117</pqid></control><display><type>article</type><title>Identification as β-Adducin of a Protein Interacting with Rabphilin-3A in the Presence of Ca2+ and Phosphatidylserine</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Miyazaki, M. ; Shirataki, H. ; Kohno, H. ; Kaibuchi, K. ; Tsugita, A. ; Takai, Y.</creator><creatorcontrib>Miyazaki, M. ; Shirataki, H. ; Kohno, H. ; Kaibuchi, K. ; Tsugita, A. ; Takai, Y.</creatorcontrib><description>Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein implicated in neurotransmitter release. We have previously identified a Rabphilin-3A-interacting protein with a Mr of about 115 kDa in bovine brain. We have attempted here to purify this protein and to determine its primary structure. Amino acid sequence analysis has revealed that this protein is a bovine counterpart of human β-adducin which is known to be a good substrate for protein kinase C. The Rabphilin-3A-interacting protein also binds to protein kinase C in the presence of Ca2+ and phosphatidylserine. These results indicate that Rabphilin-3A binds to β-adducin in the presence of Ca2+ and phosphatidylserine.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1994.2688</identifier><identifier>PMID: 7999065</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adaptor Proteins, Signal Transducing ; Amino Acid Sequence ; Animals ; Brain - metabolism ; Calcium - metabolism ; Calmodulin-Binding Proteins - metabolism ; Cattle ; GTP-Binding Proteins - chemistry ; GTP-Binding Proteins - metabolism ; Humans ; Molecular Sequence Data ; Nerve Tissue Proteins - chemistry ; Nerve Tissue Proteins - metabolism ; Phosphatidylserines - metabolism ; Protein Conformation ; rab GTP-Binding Proteins ; Rabphilin-3A ; Rats ; Sequence Homology, Amino Acid ; Vesicular Transport Proteins</subject><ispartof>Biochemical and biophysical research communications, 1994-11, Vol.205 (1), p.460-466</ispartof><rights>1994 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c271t-70daf0a174977ea867ea5b0306550b3c2385f22e4fc2c9dfc9ad134422a883d23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1994.2688$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7999065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyazaki, M.</creatorcontrib><creatorcontrib>Shirataki, H.</creatorcontrib><creatorcontrib>Kohno, H.</creatorcontrib><creatorcontrib>Kaibuchi, K.</creatorcontrib><creatorcontrib>Tsugita, A.</creatorcontrib><creatorcontrib>Takai, Y.</creatorcontrib><title>Identification as β-Adducin of a Protein Interacting with Rabphilin-3A in the Presence of Ca2+ and Phosphatidylserine</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein implicated in neurotransmitter release. We have previously identified a Rabphilin-3A-interacting protein with a Mr of about 115 kDa in bovine brain. We have attempted here to purify this protein and to determine its primary structure. Amino acid sequence analysis has revealed that this protein is a bovine counterpart of human β-adducin which is known to be a good substrate for protein kinase C. The Rabphilin-3A-interacting protein also binds to protein kinase C in the presence of Ca2+ and phosphatidylserine. These results indicate that Rabphilin-3A binds to β-adducin in the presence of Ca2+ and phosphatidylserine.</description><subject>Adaptor Proteins, Signal Transducing</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Calcium - metabolism</subject><subject>Calmodulin-Binding Proteins - metabolism</subject><subject>Cattle</subject><subject>GTP-Binding Proteins - chemistry</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Nerve Tissue Proteins - chemistry</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Phosphatidylserines - metabolism</subject><subject>Protein Conformation</subject><subject>rab GTP-Binding Proteins</subject><subject>Rabphilin-3A</subject><subject>Rats</subject><subject>Sequence Homology, Amino Acid</subject><subject>Vesicular Transport Proteins</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKAzEUhoMotVa37oSs3MjUJDOdmSxL8VIoWETBXcgkZ5xIm6lJptLX8kF8JjO0uHOTC-c7_0k-hC4pGVNC8tuqcmpMOc_GLC_LIzSkhJOEUZIdoyGJRMI4fTtFZ95_EEJplvMBGhScc5JPhmg712CDqY2SwbQWS49_vpOp1p0yFrc1lnjp2gDxMrcBnFTB2Hf8ZUKDn2W1aczK2CSd4giEBiIMHqyCvnUm2Q2WVuNl0_pNEwfo3cqDMxbO0Ukt4_nisI_Q6_3dy-wxWTw9zGfTRaJYQUNSEC1rImmR8aIAWeZxmVQkjU-fkCpVLC0nNWOQ1YoprmvFpaZpljEmyzLVLB2h633uxrWfHfgg1sYrWK2khbbzoshLTiktIjjeg8q13juoxcaZtXQ7QYnoRYtetOhFi150bLg6JHfVGvQffjAb6-W-DvF7WwNOeGV6M9o4UEHo1vwX_QtENY2C</recordid><startdate>19941130</startdate><enddate>19941130</enddate><creator>Miyazaki, M.</creator><creator>Shirataki, H.</creator><creator>Kohno, H.</creator><creator>Kaibuchi, K.</creator><creator>Tsugita, A.</creator><creator>Takai, Y.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941130</creationdate><title>Identification as β-Adducin of a Protein Interacting with Rabphilin-3A in the Presence of Ca2+ and Phosphatidylserine</title><author>Miyazaki, M. ; Shirataki, H. ; Kohno, H. ; Kaibuchi, K. ; Tsugita, A. ; Takai, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c271t-70daf0a174977ea867ea5b0306550b3c2385f22e4fc2c9dfc9ad134422a883d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adaptor Proteins, Signal Transducing</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Calcium - metabolism</topic><topic>Calmodulin-Binding Proteins - metabolism</topic><topic>Cattle</topic><topic>GTP-Binding Proteins - chemistry</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Nerve Tissue Proteins - chemistry</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Phosphatidylserines - metabolism</topic><topic>Protein Conformation</topic><topic>rab GTP-Binding Proteins</topic><topic>Rabphilin-3A</topic><topic>Rats</topic><topic>Sequence Homology, Amino Acid</topic><topic>Vesicular Transport Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyazaki, M.</creatorcontrib><creatorcontrib>Shirataki, H.</creatorcontrib><creatorcontrib>Kohno, H.</creatorcontrib><creatorcontrib>Kaibuchi, K.</creatorcontrib><creatorcontrib>Tsugita, A.</creatorcontrib><creatorcontrib>Takai, Y.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyazaki, M.</au><au>Shirataki, H.</au><au>Kohno, H.</au><au>Kaibuchi, K.</au><au>Tsugita, A.</au><au>Takai, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification as β-Adducin of a Protein Interacting with Rabphilin-3A in the Presence of Ca2+ and Phosphatidylserine</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-11-30</date><risdate>1994</risdate><volume>205</volume><issue>1</issue><spage>460</spage><epage>466</epage><pages>460-466</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein implicated in neurotransmitter release. We have previously identified a Rabphilin-3A-interacting protein with a Mr of about 115 kDa in bovine brain. We have attempted here to purify this protein and to determine its primary structure. Amino acid sequence analysis has revealed that this protein is a bovine counterpart of human β-adducin which is known to be a good substrate for protein kinase C. The Rabphilin-3A-interacting protein also binds to protein kinase C in the presence of Ca2+ and phosphatidylserine. These results indicate that Rabphilin-3A binds to β-adducin in the presence of Ca2+ and phosphatidylserine.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7999065</pmid><doi>10.1006/bbrc.1994.2688</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1994-11, Vol.205 (1), p.460-466 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76891117 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Brain - metabolism Calcium - metabolism Calmodulin-Binding Proteins - metabolism Cattle GTP-Binding Proteins - chemistry GTP-Binding Proteins - metabolism Humans Molecular Sequence Data Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - metabolism Phosphatidylserines - metabolism Protein Conformation rab GTP-Binding Proteins Rabphilin-3A Rats Sequence Homology, Amino Acid Vesicular Transport Proteins |
| title | Identification as β-Adducin of a Protein Interacting with Rabphilin-3A in the Presence of Ca2+ and Phosphatidylserine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T08%3A29%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20as%20%CE%B2-Adducin%20of%20a%20Protein%20Interacting%20with%20Rabphilin-3A%20in%20the%20Presence%20of%20Ca2+%20and%20Phosphatidylserine&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Miyazaki,%20M.&rft.date=1994-11-30&rft.volume=205&rft.issue=1&rft.spage=460&rft.epage=466&rft.pages=460-466&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1006/bbrc.1994.2688&rft_dat=%3Cproquest_cross%3E76891117%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76891117&rft_id=info:pmid/7999065&rft_els_id=S0006291X8472688X&rfr_iscdi=true |