A single-step isolation of K99 pili from B-44 strain of Escherichia coli

A single-step procedure has been developed to isolate pili from enterotoxigenic Escherichia coli strain B44 of calf origin. Pili, removed from the bacteria by heat shock treatment, were allowed to aggregate at 4°C for 16 h. The precipitated pili, isolated by centrifugation, had (a) typical pili morp...

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Veröffentlicht in:	Analytical biochemistry 1986-05, Vol.155 (1), p.51-55
Hauptverfasser:	Karkhanis, Yashwant D., Bhogal, Balbir S.
Format:	Artikel
Sprache:	eng
Schlagworte:	adhesion factor Adhesiveness Amino Acids - analysis Animals Antigens, Bacterial - isolation & purification Applied sciences Bacteriology Biological and medical sciences Cell Fractionation - methods Electrophoresis, Polyacrylamide Gel enterotoxigenicity Escherichia coli Escherichia coli - immunology Escherichia coli - ultrastructure Exact sciences and technology fimbriae Fimbriae, Bacterial - analysis Fimbriae, Bacterial - ultrastructure Fundamental and applied biological sciences. Psychology In Vitro Techniques Intestine, Small - microbiology membrane component Microbiology Microscopy, Electron Microvilli - microbiology Morphology, structure, chemical composition Other techniques and industries pili protein proteins Swine
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