A single-step isolation of K99 pili from B-44 strain of Escherichia coli
A single-step procedure has been developed to isolate pili from enterotoxigenic Escherichia coli strain B44 of calf origin. Pili, removed from the bacteria by heat shock treatment, were allowed to aggregate at 4°C for 16 h. The precipitated pili, isolated by centrifugation, had (a) typical pili morp...
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| Veröffentlicht in: | Analytical biochemistry 1986-05, Vol.155 (1), p.51-55 |
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| container_title | Analytical biochemistry |
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| creator | Karkhanis, Yashwant D. Bhogal, Balbir S. |
| description | A single-step procedure has been developed to isolate pili from enterotoxigenic
Escherichia coli strain B44 of calf origin. Pili, removed from the bacteria by heat shock treatment, were allowed to aggregate at 4°C for 16 h. The precipitated pili, isolated by centrifugation, had (a) typical pili morphology as shown by electron microscopy; (b) ability to bind pig brush border; (c) molecular weight greater than 6 million; and (d) predominance of hydrophobic amino acids. On sodium dodecyl sulfate gel electrophoresis, the subunit pilin migrated as a single polypeptide of molecular weight 17,000. |
| doi_str_mv | 10.1016/0003-2697(86)90223-X |
| format | Article |
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Escherichia coli strain B44 of calf origin. Pili, removed from the bacteria by heat shock treatment, were allowed to aggregate at 4°C for 16 h. The precipitated pili, isolated by centrifugation, had (a) typical pili morphology as shown by electron microscopy; (b) ability to bind pig brush border; (c) molecular weight greater than 6 million; and (d) predominance of hydrophobic amino acids. On sodium dodecyl sulfate gel electrophoresis, the subunit pilin migrated as a single polypeptide of molecular weight 17,000.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(86)90223-X</identifier><identifier>PMID: 2872836</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>adhesion factor ; Adhesiveness ; Amino Acids - analysis ; Animals ; Antigens, Bacterial - isolation & purification ; Applied sciences ; Bacteriology ; Biological and medical sciences ; Cell Fractionation - methods ; Electrophoresis, Polyacrylamide Gel ; enterotoxigenicity ; Escherichia coli ; Escherichia coli - immunology ; Escherichia coli - ultrastructure ; Exact sciences and technology ; fimbriae ; Fimbriae, Bacterial - analysis ; Fimbriae, Bacterial - ultrastructure ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Intestine, Small - microbiology ; membrane component ; Microbiology ; Microscopy, Electron ; Microvilli - microbiology ; Morphology, structure, chemical composition ; Other techniques and industries ; pili ; protein ; proteins ; Swine</subject><ispartof>Analytical biochemistry, 1986-05, Vol.155 (1), p.51-55</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-3a79ba8fb03c494e6c43d1d07972b6063b4b4cd0afe18d2fda3df13157bcfc333</citedby><cites>FETCH-LOGICAL-c446t-3a79ba8fb03c494e6c43d1d07972b6063b4b4cd0afe18d2fda3df13157bcfc333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(86)90223-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8109647$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8116225$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2872836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karkhanis, Yashwant D.</creatorcontrib><creatorcontrib>Bhogal, Balbir S.</creatorcontrib><title>A single-step isolation of K99 pili from B-44 strain of Escherichia coli</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A single-step procedure has been developed to isolate pili from enterotoxigenic
Escherichia coli strain B44 of calf origin. Pili, removed from the bacteria by heat shock treatment, were allowed to aggregate at 4°C for 16 h. The precipitated pili, isolated by centrifugation, had (a) typical pili morphology as shown by electron microscopy; (b) ability to bind pig brush border; (c) molecular weight greater than 6 million; and (d) predominance of hydrophobic amino acids. On sodium dodecyl sulfate gel electrophoresis, the subunit pilin migrated as a single polypeptide of molecular weight 17,000.</description><subject>adhesion factor</subject><subject>Adhesiveness</subject><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Antigens, Bacterial - isolation & purification</subject><subject>Applied sciences</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Fractionation - methods</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>enterotoxigenicity</subject><subject>Escherichia coli</subject><subject>Escherichia coli - immunology</subject><subject>Escherichia coli - ultrastructure</subject><subject>Exact sciences and technology</subject><subject>fimbriae</subject><subject>Fimbriae, Bacterial - analysis</subject><subject>Fimbriae, Bacterial - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Intestine, Small - microbiology</subject><subject>membrane component</subject><subject>Microbiology</subject><subject>Microscopy, Electron</subject><subject>Microvilli - microbiology</subject><subject>Morphology, structure, chemical composition</subject><subject>Other techniques and industries</subject><subject>pili</subject><subject>protein</subject><subject>proteins</subject><subject>Swine</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctKAzEUhoMoWi9voJCFiC5Gc5tMshFqqRcsuFHoLmRysZHppCZTwbd3akuXujqL_zs_h-8AcIrRNUaY3yCEaEG4rC4Fv5KIEFpMd8AAI8kLRJHcBYMtcgAOc_5ACGNW8n2wT0RFBOUD8DiEObTvjSty5xYw5NjoLsQWRg-fpYSL0AToU5zDu4IxmLukw284zmbmUjCzoKGJTTgGe1432Z1s5hF4ux-_jh6LycvD02g4KQxjvCuormStha8RNUwyxw2jFltUyYrUHHFas5oZi7R3WFjirabWY4rLqjbeUEqPwMW6d5Hi59LlTs1DNq5pdOviMquKCyG5KP8FMWNY9s09yNagSTHn5LxapDDX6VthpFam1UqjWmlUgqtf02rar51t-pf13Nnt0kZtn59vcp2NbnzSrQl5iwmMOSHl_1j_Tlb12O0ac73br-CSyia41jgbkjOdsjH8fe4PLwakkA</recordid><startdate>19860515</startdate><enddate>19860515</enddate><creator>Karkhanis, Yashwant D.</creator><creator>Bhogal, Balbir S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19860515</creationdate><title>A single-step isolation of K99 pili from B-44 strain of Escherichia coli</title><author>Karkhanis, Yashwant D. ; Bhogal, Balbir S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-3a79ba8fb03c494e6c43d1d07972b6063b4b4cd0afe18d2fda3df13157bcfc333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>adhesion factor</topic><topic>Adhesiveness</topic><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Antigens, Bacterial - isolation & purification</topic><topic>Applied sciences</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Fractionation - methods</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>enterotoxigenicity</topic><topic>Escherichia coli</topic><topic>Escherichia coli - immunology</topic><topic>Escherichia coli - ultrastructure</topic><topic>Exact sciences and technology</topic><topic>fimbriae</topic><topic>Fimbriae, Bacterial - analysis</topic><topic>Fimbriae, Bacterial - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Intestine, Small - microbiology</topic><topic>membrane component</topic><topic>Microbiology</topic><topic>Microscopy, Electron</topic><topic>Microvilli - microbiology</topic><topic>Morphology, structure, chemical composition</topic><topic>Other techniques and industries</topic><topic>pili</topic><topic>protein</topic><topic>proteins</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karkhanis, Yashwant D.</creatorcontrib><creatorcontrib>Bhogal, Balbir S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karkhanis, Yashwant D.</au><au>Bhogal, Balbir S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A single-step isolation of K99 pili from B-44 strain of Escherichia coli</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1986-05-15</date><risdate>1986</risdate><volume>155</volume><issue>1</issue><spage>51</spage><epage>55</epage><pages>51-55</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A single-step procedure has been developed to isolate pili from enterotoxigenic
Escherichia coli strain B44 of calf origin. Pili, removed from the bacteria by heat shock treatment, were allowed to aggregate at 4°C for 16 h. The precipitated pili, isolated by centrifugation, had (a) typical pili morphology as shown by electron microscopy; (b) ability to bind pig brush border; (c) molecular weight greater than 6 million; and (d) predominance of hydrophobic amino acids. On sodium dodecyl sulfate gel electrophoresis, the subunit pilin migrated as a single polypeptide of molecular weight 17,000.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2872836</pmid><doi>10.1016/0003-2697(86)90223-X</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1986-05, Vol.155 (1), p.51-55 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | adhesion factor Adhesiveness Amino Acids - analysis Animals Antigens, Bacterial - isolation & purification Applied sciences Bacteriology Biological and medical sciences Cell Fractionation - methods Electrophoresis, Polyacrylamide Gel enterotoxigenicity Escherichia coli Escherichia coli - immunology Escherichia coli - ultrastructure Exact sciences and technology fimbriae Fimbriae, Bacterial - analysis Fimbriae, Bacterial - ultrastructure Fundamental and applied biological sciences. Psychology In Vitro Techniques Intestine, Small - microbiology membrane component Microbiology Microscopy, Electron Microvilli - microbiology Morphology, structure, chemical composition Other techniques and industries pili protein proteins Swine |
| title | A single-step isolation of K99 pili from B-44 strain of Escherichia coli |
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