Affinity isolation of transcriptionally active DNA
Chicken erythrocyte nuclei were nick translated with the chemically cleavable biotinylated nucleotide. Bio-12-SS-dUTP. DNA was purified, digested with restriction endonucleases, and applied to an avidin-agarose affinity column. Seventy percent of the nick translated DNA bound to the column. This DNA...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1986-05, Vol.137 (1), p.474-479 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Roseman, Barry Lough, John Houkom, Everin Herman, Tim |
| description | Chicken erythrocyte nuclei were nick translated with the chemically cleavable biotinylated nucleotide. Bio-12-SS-dUTP. DNA was purified, digested with restriction endonucleases, and applied to an avidin-agarose affinity column. Seventy percent of the nick translated DNA bound to the column. This DNA was recovered from the column by chemical cleavage of the linker arm joining biotin to the DNA. Dot hybridization analysis of this DNA revealed a significant enrichment of the alpha-D-globin gene. This result suggests an approach to isolate transcriptionally active genes. |
| doi_str_mv | 10.1016/0006-291X(86)91234-9 |
| format | Article |
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Bio-12-SS-dUTP. DNA was purified, digested with restriction endonucleases, and applied to an avidin-agarose affinity column. Seventy percent of the nick translated DNA bound to the column. This DNA was recovered from the column by chemical cleavage of the linker arm joining biotin to the DNA. Dot hybridization analysis of this DNA revealed a significant enrichment of the alpha-D-globin gene. This result suggests an approach to isolate transcriptionally active genes.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(86)91234-9</identifier><identifier>PMID: 3718516</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Applied sciences ; Biotin ; Chickens ; Chromatography, Affinity - methods ; Deoxyribonuclease I ; DNA - genetics ; DNA - isolation & purification ; Erythrocytes ; Exact sciences and technology ; Gene Expression Regulation ; Genes ; Globins - genetics ; Other techniques and industries ; Transcription, Genetic</subject><ispartof>Biochemical and biophysical research communications, 1986-05, Vol.137 (1), p.474-479</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-6aa1d432f7616764978c89237fce29582b7e8e754d8393650ce11f5a0644831d3</citedby><cites>FETCH-LOGICAL-c386t-6aa1d432f7616764978c89237fce29582b7e8e754d8393650ce11f5a0644831d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(86)91234-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8185224$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3718516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roseman, Barry</creatorcontrib><creatorcontrib>Lough, John</creatorcontrib><creatorcontrib>Houkom, Everin</creatorcontrib><creatorcontrib>Herman, Tim</creatorcontrib><title>Affinity isolation of transcriptionally active DNA</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Chicken erythrocyte nuclei were nick translated with the chemically cleavable biotinylated nucleotide. 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This result suggests an approach to isolate transcriptionally active genes.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Biotin</subject><subject>Chickens</subject><subject>Chromatography, Affinity - methods</subject><subject>Deoxyribonuclease I</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Erythrocytes</subject><subject>Exact sciences and technology</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Globins - genetics</subject><subject>Other techniques and industries</subject><subject>Transcription, Genetic</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcIeRPSwmkmy-bgIpX5C0YuCt5BmE4hsd2uyLfTfu2uXHj0NzDzvMPMgdA74FjDwO4wxz4mCr2vJbxQQynJ1gMaAFc4JYHaIxnvkGJ2k9I0xAONqhEZUgCyAjxGZeh_q0G6zkJrKtKGps8ZnbTR1sjGs-oapqm1mbBs2Lnt4m56iI2-q5M6GOkGfT48fs5d8_v78OpvOc0slb3NuDJSMEi84cMGZEtJKRajw1hFVSLIQTjpRsFJSRXmBrQPwhcGcMUmhpBN0tdu7is3P2qVWL0OyrqpM7Zp10oJLKbtnO5DtQBublKLzehXD0sStBqx7Vbr3oHsPWnL9p0qrLnYx7F8vlq7chwY33fxymJtkTeU7JTakPSY7ihDWYfc7zHUuNsFFnWxwtXVliM62umzC_3f8Amwsguc</recordid><startdate>19860529</startdate><enddate>19860529</enddate><creator>Roseman, Barry</creator><creator>Lough, John</creator><creator>Houkom, Everin</creator><creator>Herman, Tim</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860529</creationdate><title>Affinity isolation of transcriptionally active DNA</title><author>Roseman, Barry ; Lough, John ; Houkom, Everin ; Herman, Tim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-6aa1d432f7616764978c89237fce29582b7e8e754d8393650ce11f5a0644831d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Biotin</topic><topic>Chickens</topic><topic>Chromatography, Affinity - methods</topic><topic>Deoxyribonuclease I</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Erythrocytes</topic><topic>Exact sciences and technology</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Globins - genetics</topic><topic>Other techniques and industries</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roseman, Barry</creatorcontrib><creatorcontrib>Lough, John</creatorcontrib><creatorcontrib>Houkom, Everin</creatorcontrib><creatorcontrib>Herman, Tim</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roseman, Barry</au><au>Lough, John</au><au>Houkom, Everin</au><au>Herman, Tim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Affinity isolation of transcriptionally active DNA</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1986-05-29</date><risdate>1986</risdate><volume>137</volume><issue>1</issue><spage>474</spage><epage>479</epage><pages>474-479</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Chicken erythrocyte nuclei were nick translated with the chemically cleavable biotinylated nucleotide. Bio-12-SS-dUTP. DNA was purified, digested with restriction endonucleases, and applied to an avidin-agarose affinity column. Seventy percent of the nick translated DNA bound to the column. This DNA was recovered from the column by chemical cleavage of the linker arm joining biotin to the DNA. Dot hybridization analysis of this DNA revealed a significant enrichment of the alpha-D-globin gene. This result suggests an approach to isolate transcriptionally active genes.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3718516</pmid><doi>10.1016/0006-291X(86)91234-9</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1986-05, Vol.137 (1), p.474-479 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76888090 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Applied sciences Biotin Chickens Chromatography, Affinity - methods Deoxyribonuclease I DNA - genetics DNA - isolation & purification Erythrocytes Exact sciences and technology Gene Expression Regulation Genes Globins - genetics Other techniques and industries Transcription, Genetic |
| title | Affinity isolation of transcriptionally active DNA |
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