16S rDNA sequence and phylogenetic position of an uncultivated spirochete from the hindgut of the termite Mastotermes darwiniensis Froggatt
We have analyzed the 16S rDNA sequence and the phylogenetic position of an uncultivated spirochete from the hindgut contents of the Australian termite Mastotermes darwiniensis Froggatt. The 16S rRNA genes of bacteria from the hindgut contents of Mastotermes darwiniensis were amplified by polymerase...
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| Veröffentlicht in: | FEMS microbiology letters 1994-11, Vol.123 (3), p.269-273 |
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| creator | Berchtold, M Ludwig, W Konig, H |
| description | We have analyzed the 16S rDNA sequence and the phylogenetic position of an uncultivated spirochete from the hindgut contents of the Australian termite Mastotermes darwiniensis Froggatt. The 16S rRNA genes of bacteria from the hindgut contents of Mastotermes darwiniensis were amplified by polymerase chain reaction. The amplification products were cloned and sequenced. The sequences were compared to known homologous primary structures. Two of the clones (MDS1 and MDS3) had an insert of 1498 nucleotides showing typical signatures of spirochete 16S rRNA sequences. The sequences of the two clones were most similar to the 16S rRNA sequence of Spirochaeta stenostrepta (89.8%) and Treponema sp. strain HI (90.7%). Phylogenetical analysis positioned the hindgut spirochete sequence with that of the free-living anaerobic Spirochaeta stenostrepta and Treponema sp. strain HI as its nearest relatives within the cluster of the spirochetes. We conclude that the analyzed SSU rDNA sequences originate from a spirochete related to the genus Treponema. It is possibly one of the uncultivated unique spirochetes symbiotic in termite hindguts. |
| doi_str_mv | 10.1111/j.1574-6968.1994.tb07235.x |
| format | Article |
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The 16S rRNA genes of bacteria from the hindgut contents of Mastotermes darwiniensis were amplified by polymerase chain reaction. The amplification products were cloned and sequenced. The sequences were compared to known homologous primary structures. Two of the clones (MDS1 and MDS3) had an insert of 1498 nucleotides showing typical signatures of spirochete 16S rRNA sequences. The sequences of the two clones were most similar to the 16S rRNA sequence of Spirochaeta stenostrepta (89.8%) and Treponema sp. strain HI (90.7%). Phylogenetical analysis positioned the hindgut spirochete sequence with that of the free-living anaerobic Spirochaeta stenostrepta and Treponema sp. strain HI as its nearest relatives within the cluster of the spirochetes. We conclude that the analyzed SSU rDNA sequences originate from a spirochete related to the genus Treponema. It is possibly one of the uncultivated unique spirochetes symbiotic in termite hindguts.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1994.tb07235.x</identifier><identifier>PMID: 7527363</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>16S rRNA sequence ; Animal, plant and microbial ecology ; Animals ; Bacteriology ; Base Sequence ; Biological and medical sciences ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; genbank/x79548 ; hindgut ; Insecta - microbiology ; intestinal microorganisms ; Isoptera ; Microbial ecology ; Microbiology ; Molecular Sequence Data ; Normal microflora of man and animals. Rumen ; nucleotide sequences ; Phylogeny ; ribosomal DNA ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; Spirochaeta stenostrepta ; Spirochaetaceae ; Spirochaetales - genetics ; Spirochete ; Systematics ; Termite ; Treponema</subject><ispartof>FEMS microbiology letters, 1994-11, Vol.123 (3), p.269-273</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4229-e1a600fa98b445c6c572e449d2a02bc386a8d1076f016423e462da44b3d20543</citedby><cites>FETCH-LOGICAL-c4229-e1a600fa98b445c6c572e449d2a02bc386a8d1076f016423e462da44b3d20543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1994.tb07235.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1994.tb07235.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3321415$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7527363$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Berchtold, M</creatorcontrib><creatorcontrib>Ludwig, W</creatorcontrib><creatorcontrib>Konig, H</creatorcontrib><title>16S rDNA sequence and phylogenetic position of an uncultivated spirochete from the hindgut of the termite Mastotermes darwiniensis Froggatt</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>We have analyzed the 16S rDNA sequence and the phylogenetic position of an uncultivated spirochete from the hindgut contents of the Australian termite Mastotermes darwiniensis Froggatt. The 16S rRNA genes of bacteria from the hindgut contents of Mastotermes darwiniensis were amplified by polymerase chain reaction. The amplification products were cloned and sequenced. The sequences were compared to known homologous primary structures. Two of the clones (MDS1 and MDS3) had an insert of 1498 nucleotides showing typical signatures of spirochete 16S rRNA sequences. The sequences of the two clones were most similar to the 16S rRNA sequence of Spirochaeta stenostrepta (89.8%) and Treponema sp. strain HI (90.7%). Phylogenetical analysis positioned the hindgut spirochete sequence with that of the free-living anaerobic Spirochaeta stenostrepta and Treponema sp. strain HI as its nearest relatives within the cluster of the spirochetes. We conclude that the analyzed SSU rDNA sequences originate from a spirochete related to the genus Treponema. It is possibly one of the uncultivated unique spirochetes symbiotic in termite hindguts.</description><subject>16S rRNA sequence</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/x79548</subject><subject>hindgut</subject><subject>Insecta - microbiology</subject><subject>intestinal microorganisms</subject><subject>Isoptera</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Normal microflora of man and animals. Rumen</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>ribosomal DNA</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Spirochaeta stenostrepta</subject><subject>Spirochaetaceae</subject><subject>Spirochaetales - genetics</subject><subject>Spirochete</subject><subject>Systematics</subject><subject>Termite</subject><subject>Treponema</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc1u1DAUhSMEKkPhERAWQuwS_BcnYYFUFaYgTWHRsrYc5ybjUWIH26GdZ-Clm2ii2eONdXW-e699TpK8Jzgj8_l0yEhe8FRUosxIVfEs1rigLM8enyWbs_Q82WBWlCnBVfEyeRXCAWPMKRYXyUWR04IJtkn-EXGH_NefVyjAnwmsBqRsg8b9sXcdWIhGo9EFE42zyLWziCarpz6avypCg8JovNN7iIBa7wYU94D2xjbdFBd8KSP4wcz6rQrRLQUE1Cj_YKwBG0xAW--6TsX4OnnRqj7Am_W-TO633-6vv6e7Xzc_rq92qeaUVikQJTBuVVXWnOda6LygwHnVUIVprVkpVNkQXIgWE8EpAy5oozivWUNxztll8vE0dvRu_nKIcjBBQ98rC24KshBlSUlVzuDnE6i9C8FDK0dvBuWPkmC5BCEPcnFbLm7LJQi5BiEf5-a365apHqA5t67Oz_qHVVdBq771ymoTzhhjlHCSz9iXE_Zgejj-xwPk9nZHRTUPeHca0ConVefnHb_vKCYME14JRhl7Agn9sGU</recordid><startdate>199411</startdate><enddate>199411</enddate><creator>Berchtold, M</creator><creator>Ludwig, W</creator><creator>Konig, H</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199411</creationdate><title>16S rDNA sequence and phylogenetic position of an uncultivated spirochete from the hindgut of the termite Mastotermes darwiniensis Froggatt</title><author>Berchtold, M ; Ludwig, W ; Konig, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4229-e1a600fa98b445c6c572e449d2a02bc386a8d1076f016423e462da44b3d20543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>16S rRNA sequence</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/x79548</topic><topic>hindgut</topic><topic>Insecta - microbiology</topic><topic>intestinal microorganisms</topic><topic>Isoptera</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Normal microflora of man and animals. Rumen</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>ribosomal DNA</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Spirochaeta stenostrepta</topic><topic>Spirochaetaceae</topic><topic>Spirochaetales - genetics</topic><topic>Spirochete</topic><topic>Systematics</topic><topic>Termite</topic><topic>Treponema</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Berchtold, M</creatorcontrib><creatorcontrib>Ludwig, W</creatorcontrib><creatorcontrib>Konig, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Berchtold, M</au><au>Ludwig, W</au><au>Konig, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>16S rDNA sequence and phylogenetic position of an uncultivated spirochete from the hindgut of the termite Mastotermes darwiniensis Froggatt</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1994-11</date><risdate>1994</risdate><volume>123</volume><issue>3</issue><spage>269</spage><epage>273</epage><pages>269-273</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>We have analyzed the 16S rDNA sequence and the phylogenetic position of an uncultivated spirochete from the hindgut contents of the Australian termite Mastotermes darwiniensis Froggatt. The 16S rRNA genes of bacteria from the hindgut contents of Mastotermes darwiniensis were amplified by polymerase chain reaction. The amplification products were cloned and sequenced. The sequences were compared to known homologous primary structures. Two of the clones (MDS1 and MDS3) had an insert of 1498 nucleotides showing typical signatures of spirochete 16S rRNA sequences. The sequences of the two clones were most similar to the 16S rRNA sequence of Spirochaeta stenostrepta (89.8%) and Treponema sp. strain HI (90.7%). Phylogenetical analysis positioned the hindgut spirochete sequence with that of the free-living anaerobic Spirochaeta stenostrepta and Treponema sp. strain HI as its nearest relatives within the cluster of the spirochetes. We conclude that the analyzed SSU rDNA sequences originate from a spirochete related to the genus Treponema. It is possibly one of the uncultivated unique spirochetes symbiotic in termite hindguts.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7527363</pmid><doi>10.1111/j.1574-6968.1994.tb07235.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | FEMS microbiology letters, 1994-11, Vol.123 (3), p.269-273 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection; Oxford University Press Journals Digital Archive Legacy |
| subjects | 16S rRNA sequence Animal, plant and microbial ecology Animals Bacteriology Base Sequence Biological and medical sciences DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology genbank/x79548 hindgut Insecta - microbiology intestinal microorganisms Isoptera Microbial ecology Microbiology Molecular Sequence Data Normal microflora of man and animals. Rumen nucleotide sequences Phylogeny ribosomal DNA RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Spirochaeta stenostrepta Spirochaetaceae Spirochaetales - genetics Spirochete Systematics Termite Treponema |
| title | 16S rDNA sequence and phylogenetic position of an uncultivated spirochete from the hindgut of the termite Mastotermes darwiniensis Froggatt |
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