Behavior of intracellular glutathione during platelet thromboxane synthesis in diabetes
The time-dependent relationship between the levels of the reduced form of glutathione (GSH) and thromboxane A 2 (TXA 2) synthesis, as measured by the accumulation of TXB 2, in platelets from human diabetic and control subjects was investigated during aggregation. In platelets from control subjects,...
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| Veröffentlicht in: | Prostaglandins leukotrienes and medicine 1986-04, Vol.22 (1), p.117-128 |
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| creator | Thomas, George Lucas, Fred V. Peter Schumacher, O. Skrinska, Victor |
| description | The time-dependent relationship between the levels of the reduced form of glutathione (GSH) and thromboxane A
2 (TXA
2) synthesis, as measured by the accumulation of TXB
2, in platelets from human diabetic and control subjects was investigated during aggregation. In platelets from control subjects, the GSH level decreased to 21% of the initial level within 30 sec in response to arachidonic acid (1.65 mm) and rapidly recovered to 91% by 1 min. In platelets from diabetic subjects, the GSH level decreased to 3% of the initial level within 30 sec and recovered to only 41% by 1 min. During collagen (20 ug/ml) aggregation, platelets from control subjects had a 15 sec lag phase which was followed by a decrease in the GSH level to 21% of the initial level within 1 min and a recovery to 74% by 2 min. Platelets from diabetic subjects in response to collagen showed no lag phase and decreased to 10% of the initial level within 1 min which was followed by a recovery to 34% by 2 min. In all aggregations, the initial GSH level was significantly (p |
| doi_str_mv | 10.1016/0262-1746(86)90028-4 |
| format | Article |
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2 (TXA
2) synthesis, as measured by the accumulation of TXB
2, in platelets from human diabetic and control subjects was investigated during aggregation. In platelets from control subjects, the GSH level decreased to 21% of the initial level within 30 sec in response to arachidonic acid (1.65 mm) and rapidly recovered to 91% by 1 min. In platelets from diabetic subjects, the GSH level decreased to 3% of the initial level within 30 sec and recovered to only 41% by 1 min. During collagen (20 ug/ml) aggregation, platelets from control subjects had a 15 sec lag phase which was followed by a decrease in the GSH level to 21% of the initial level within 1 min and a recovery to 74% by 2 min. Platelets from diabetic subjects in response to collagen showed no lag phase and decreased to 10% of the initial level within 1 min which was followed by a recovery to 34% by 2 min. In all aggregations, the initial GSH level was significantly (p<.001) lower in platelets from diabetic subjects and remained significantly (p<.01) lower than GSH in platelets from control subjects throughout the aggregation. The amount of TXB
2 formed by platelets from control subjects reached a maximum in response to arachidonic acid and collagen by 1 min and 2 min, respectively, whereas, the TXB
2 continued to increase up to 4 min when platelets from diabetic subjects were aggregated. These data indicate that TXA
2 synthesis occurs during the decrease in GSH and ceases when the GSH level recovers. The continued synthesis of TXA
2 by platelets from diabetic subjects coincides with the gradual recovery of the GSH level.</description><identifier>ISSN: 0262-1746</identifier><identifier>DOI: 10.1016/0262-1746(86)90028-4</identifier><identifier>PMID: 3459196</identifier><language>eng</language><publisher>Edinburgh: Elsevier Ltd</publisher><subject>Arachidonic Acids - pharmacology ; Biological and medical sciences ; Blood Platelets - metabolism ; Blood Platelets - physiopathology ; Collagen - pharmacology ; Diabetes Mellitus, Type 1 - blood ; Diabetes Mellitus, Type 1 - physiopathology ; Diabetes Mellitus, Type 2 - blood ; Diabetes Mellitus, Type 2 - physiopathology ; Diabetes. Impaired glucose tolerance ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Female ; Glutathione - blood ; Glutathione - metabolism ; Humans ; Male ; Medical sciences ; Middle Aged ; Platelet Aggregation - drug effects ; Thromboxane A2 - biosynthesis ; Time Factors</subject><ispartof>Prostaglandins leukotrienes and medicine, 1986-04, Vol.22 (1), p.117-128</ispartof><rights>1986</rights><rights>1986 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-9a13e863497588c27f791517952fc2a52ee8ee0bdc1cae28fa48e2be28a2e2b13</citedby><cites>FETCH-LOGICAL-c386t-9a13e863497588c27f791517952fc2a52ee8ee0bdc1cae28fa48e2be28a2e2b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8634251$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3459196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomas, George</creatorcontrib><creatorcontrib>Lucas, Fred V.</creatorcontrib><creatorcontrib>Peter Schumacher, O.</creatorcontrib><creatorcontrib>Skrinska, Victor</creatorcontrib><title>Behavior of intracellular glutathione during platelet thromboxane synthesis in diabetes</title><title>Prostaglandins leukotrienes and medicine</title><addtitle>Prostaglandins Leukot Med</addtitle><description>The time-dependent relationship between the levels of the reduced form of glutathione (GSH) and thromboxane A
2 (TXA
2) synthesis, as measured by the accumulation of TXB
2, in platelets from human diabetic and control subjects was investigated during aggregation. In platelets from control subjects, the GSH level decreased to 21% of the initial level within 30 sec in response to arachidonic acid (1.65 mm) and rapidly recovered to 91% by 1 min. In platelets from diabetic subjects, the GSH level decreased to 3% of the initial level within 30 sec and recovered to only 41% by 1 min. During collagen (20 ug/ml) aggregation, platelets from control subjects had a 15 sec lag phase which was followed by a decrease in the GSH level to 21% of the initial level within 1 min and a recovery to 74% by 2 min. Platelets from diabetic subjects in response to collagen showed no lag phase and decreased to 10% of the initial level within 1 min which was followed by a recovery to 34% by 2 min. In all aggregations, the initial GSH level was significantly (p<.001) lower in platelets from diabetic subjects and remained significantly (p<.01) lower than GSH in platelets from control subjects throughout the aggregation. The amount of TXB
2 formed by platelets from control subjects reached a maximum in response to arachidonic acid and collagen by 1 min and 2 min, respectively, whereas, the TXB
2 continued to increase up to 4 min when platelets from diabetic subjects were aggregated. These data indicate that TXA
2 synthesis occurs during the decrease in GSH and ceases when the GSH level recovers. The continued synthesis of TXA
2 by platelets from diabetic subjects coincides with the gradual recovery of the GSH level.</description><subject>Arachidonic Acids - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blood Platelets - metabolism</subject><subject>Blood Platelets - physiopathology</subject><subject>Collagen - pharmacology</subject><subject>Diabetes Mellitus, Type 1 - blood</subject><subject>Diabetes Mellitus, Type 1 - physiopathology</subject><subject>Diabetes Mellitus, Type 2 - blood</subject><subject>Diabetes Mellitus, Type 2 - physiopathology</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Female</subject><subject>Glutathione - blood</subject><subject>Glutathione - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Platelet Aggregation - drug effects</subject><subject>Thromboxane A2 - biosynthesis</subject><subject>Time Factors</subject><issn>0262-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxXNQdF39Bgo9iOih2qRpml4EFf-B4EXxGKbpdDfSbdYkXfTbm7rLHj3NMO_N8OZHyDHNLmlGxVXGBEtpycW5FBdVljGZ8h0y2Y73yYH3n-OcC7FH9nJeVLQSE_Jxi3NYGesS2yamDw40dt3QgUtm3RAgzI3tMWkGZ_pZsuwgYIchCXNnF7X9hqj5nz7M0Rsf95PGQI0B_SHZbaHzeLSpU_L-cP9295S-vD4-3928pDqXIqQV0BylyHlVFlJqVrZlRQtaVgVrNYOCIUrErG401YBMtsAlsjp2wGKl-ZScre8unf0a0Ae1MH58ISazg1elkCXnWR6NfG3UznrvsFVLZxbgfhTN1MhQjbDUCEtJof4YKh7XTjb3h3qBzXZpAzDqpxsdvIauddBr47e28TVWjDGv1zaMLFYGnfLaYK-xMQ51UI01_-f4BTIRkOY</recordid><startdate>19860401</startdate><enddate>19860401</enddate><creator>Thomas, George</creator><creator>Lucas, Fred V.</creator><creator>Peter Schumacher, O.</creator><creator>Skrinska, Victor</creator><general>Elsevier Ltd</general><general>Churchill Livingstone</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860401</creationdate><title>Behavior of intracellular glutathione during platelet thromboxane synthesis in diabetes</title><author>Thomas, George ; Lucas, Fred V. ; Peter Schumacher, O. ; Skrinska, Victor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-9a13e863497588c27f791517952fc2a52ee8ee0bdc1cae28fa48e2be28a2e2b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Arachidonic Acids - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blood Platelets - metabolism</topic><topic>Blood Platelets - physiopathology</topic><topic>Collagen - pharmacology</topic><topic>Diabetes Mellitus, Type 1 - blood</topic><topic>Diabetes Mellitus, Type 1 - physiopathology</topic><topic>Diabetes Mellitus, Type 2 - blood</topic><topic>Diabetes Mellitus, Type 2 - physiopathology</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Female</topic><topic>Glutathione - blood</topic><topic>Glutathione - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Platelet Aggregation - drug effects</topic><topic>Thromboxane A2 - biosynthesis</topic><topic>Time Factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Thomas, George</creatorcontrib><creatorcontrib>Lucas, Fred V.</creatorcontrib><creatorcontrib>Peter Schumacher, O.</creatorcontrib><creatorcontrib>Skrinska, Victor</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Prostaglandins leukotrienes and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomas, George</au><au>Lucas, Fred V.</au><au>Peter Schumacher, O.</au><au>Skrinska, Victor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Behavior of intracellular glutathione during platelet thromboxane synthesis in diabetes</atitle><jtitle>Prostaglandins leukotrienes and medicine</jtitle><addtitle>Prostaglandins Leukot Med</addtitle><date>1986-04-01</date><risdate>1986</risdate><volume>22</volume><issue>1</issue><spage>117</spage><epage>128</epage><pages>117-128</pages><issn>0262-1746</issn><abstract>The time-dependent relationship between the levels of the reduced form of glutathione (GSH) and thromboxane A
2 (TXA
2) synthesis, as measured by the accumulation of TXB
2, in platelets from human diabetic and control subjects was investigated during aggregation. In platelets from control subjects, the GSH level decreased to 21% of the initial level within 30 sec in response to arachidonic acid (1.65 mm) and rapidly recovered to 91% by 1 min. In platelets from diabetic subjects, the GSH level decreased to 3% of the initial level within 30 sec and recovered to only 41% by 1 min. During collagen (20 ug/ml) aggregation, platelets from control subjects had a 15 sec lag phase which was followed by a decrease in the GSH level to 21% of the initial level within 1 min and a recovery to 74% by 2 min. Platelets from diabetic subjects in response to collagen showed no lag phase and decreased to 10% of the initial level within 1 min which was followed by a recovery to 34% by 2 min. In all aggregations, the initial GSH level was significantly (p<.001) lower in platelets from diabetic subjects and remained significantly (p<.01) lower than GSH in platelets from control subjects throughout the aggregation. The amount of TXB
2 formed by platelets from control subjects reached a maximum in response to arachidonic acid and collagen by 1 min and 2 min, respectively, whereas, the TXB
2 continued to increase up to 4 min when platelets from diabetic subjects were aggregated. These data indicate that TXA
2 synthesis occurs during the decrease in GSH and ceases when the GSH level recovers. The continued synthesis of TXA
2 by platelets from diabetic subjects coincides with the gradual recovery of the GSH level.</abstract><cop>Edinburgh</cop><cop>New York, NY</cop><pub>Elsevier Ltd</pub><pmid>3459196</pmid><doi>10.1016/0262-1746(86)90028-4</doi><tpages>12</tpages></addata></record> |
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| subjects | Arachidonic Acids - pharmacology Biological and medical sciences Blood Platelets - metabolism Blood Platelets - physiopathology Collagen - pharmacology Diabetes Mellitus, Type 1 - blood Diabetes Mellitus, Type 1 - physiopathology Diabetes Mellitus, Type 2 - blood Diabetes Mellitus, Type 2 - physiopathology Diabetes. Impaired glucose tolerance Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Female Glutathione - blood Glutathione - metabolism Humans Male Medical sciences Middle Aged Platelet Aggregation - drug effects Thromboxane A2 - biosynthesis Time Factors |
| title | Behavior of intracellular glutathione during platelet thromboxane synthesis in diabetes |
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