Increase in bcl-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbil hippocampus
We studied the expression of bcl-2 oncoprotein in the gerbil hippocampus after transient ischemia. Immunostaining using monoclonal antibody raised against bcl-2 oncoprotein revealed intense immunoreactivity in the CA1 area following 2 min of ischemia, which induced tolerance to subsequent ischemia a...
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Veröffentlicht in: | Neuroscience research 1994-07, Vol.20 (1), p.95-99 |
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creator | Shimazaki, Kuniko Ishida, Akihiro Kawai, Nobufumi |
description | We studied the expression of bcl-2 oncoprotein in the gerbil hippocampus after transient ischemia. Immunostaining using monoclonal antibody raised against bcl-2 oncoprotein revealed intense immunoreactivity in the CA1 area following 2 min of ischemia, which induced tolerance to subsequent ischemia and prevented delayed neuronal death (DND). Following ischemia for 5 min, however, bcl-2 oncoprotein immunoreactivity was decreased, reflecting neuronal death in the CA1 area. However, pretreatment with ischemia of 2 min that prevented DND due to subsequent ischemia for 5 min, showed increased immunoreactivity. On the other hand, following 1 min of ischemia which failed to induce tolerance, no increase in the bcl-2 oncoprotein was observed. The results evidenced that expression of bcl-2 oncoprotein in the CA1 area following brief ischemia is closely related to the acquisition of resistance to DND. |
doi_str_mv | 10.1016/0168-0102(94)90026-4 |
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Immunostaining using monoclonal antibody raised against bcl-2 oncoprotein revealed intense immunoreactivity in the CA1 area following 2 min of ischemia, which induced tolerance to subsequent ischemia and prevented delayed neuronal death (DND). Following ischemia for 5 min, however, bcl-2 oncoprotein immunoreactivity was decreased, reflecting neuronal death in the CA1 area. However, pretreatment with ischemia of 2 min that prevented DND due to subsequent ischemia for 5 min, showed increased immunoreactivity. On the other hand, following 1 min of ischemia which failed to induce tolerance, no increase in the bcl-2 oncoprotein was observed. The results evidenced that expression of bcl-2 oncoprotein in the CA1 area following brief ischemia is closely related to the acquisition of resistance to DND.</description><identifier>ISSN: 0168-0102</identifier><identifier>EISSN: 1872-8111</identifier><identifier>DOI: 10.1016/0168-0102(94)90026-4</identifier><identifier>PMID: 7527133</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Antibodies, Monoclonal ; Apoptosis ; bcl-2 ; Cell Death ; Delayed neuronal death ; Gerbil ; Gerbillinae ; Hippocampus ; Hippocampus - metabolism ; Hippocampus - pathology ; Ischemia ; Ischemic Attack, Transient - pathology ; Neurons - pathology ; Oncoprotein ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-bcl-2 ; Staining and Labeling ; Time Factors ; Tolerance for neuronal death</subject><ispartof>Neuroscience research, 1994-07, Vol.20 (1), p.95-99</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-b255194c453f0833794c2ef6a5c9121fe381fc52bf9d837522508c37e111ee0b3</citedby><cites>FETCH-LOGICAL-c357t-b255194c453f0833794c2ef6a5c9121fe381fc52bf9d837522508c37e111ee0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-0102(94)90026-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7527133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimazaki, Kuniko</creatorcontrib><creatorcontrib>Ishida, Akihiro</creatorcontrib><creatorcontrib>Kawai, Nobufumi</creatorcontrib><title>Increase in bcl-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbil hippocampus</title><title>Neuroscience research</title><addtitle>Neurosci Res</addtitle><description>We studied the expression of bcl-2 oncoprotein in the gerbil hippocampus after transient ischemia. Immunostaining using monoclonal antibody raised against bcl-2 oncoprotein revealed intense immunoreactivity in the CA1 area following 2 min of ischemia, which induced tolerance to subsequent ischemia and prevented delayed neuronal death (DND). Following ischemia for 5 min, however, bcl-2 oncoprotein immunoreactivity was decreased, reflecting neuronal death in the CA1 area. However, pretreatment with ischemia of 2 min that prevented DND due to subsequent ischemia for 5 min, showed increased immunoreactivity. On the other hand, following 1 min of ischemia which failed to induce tolerance, no increase in the bcl-2 oncoprotein was observed. The results evidenced that expression of bcl-2 oncoprotein in the CA1 area following brief ischemia is closely related to the acquisition of resistance to DND.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Apoptosis</subject><subject>bcl-2</subject><subject>Cell Death</subject><subject>Delayed neuronal death</subject><subject>Gerbil</subject><subject>Gerbillinae</subject><subject>Hippocampus</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - pathology</subject><subject>Ischemia</subject><subject>Ischemic Attack, Transient - pathology</subject><subject>Neurons - pathology</subject><subject>Oncoprotein</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-bcl-2</subject><subject>Staining and Labeling</subject><subject>Time Factors</subject><subject>Tolerance for neuronal death</subject><issn>0168-0102</issn><issn>1872-8111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtr3DAUhUVJmUyn_QcNaBXShRu9bMmbQBj6CAxkk6yFLF_XCrbkSHYg_z5yZ8gyC6GL7rnn6nwIfafkJyW0us5HFYQSdlWLHzUhrCrEJ7SlSrJCUUrP0PZdco6-pPRECOG14Bu0kSWTlPMtGu68jWASYOdxY4eC4eBtmGKYIb8Y3-K5BzyHAaLxdq2wS7aH0ZnC-Xax0GIPSwzeDLgFM_er0zrzD2LjBty7aQrWjNOSvqLPnRkSfDvdO_T4-9fD_m9xuP9zt789FJaXci4aVpa0FlaUvCOKc5lrBl1lSltTRjvgina2ZE1Xt4rnKKwkynIJOTQAafgOXR59c4znBdKsx_xnGAbjISxJy0pVSkiZheIotDGkFKHTU3Sjia-aEr1C1itBvRLUtdD_IWuRxy5O_kszQvs-dKKa-zfHPuSQLw6iTtZBpte6CHbWbXAfL3gDPs-LTA</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>Shimazaki, Kuniko</creator><creator>Ishida, Akihiro</creator><creator>Kawai, Nobufumi</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940701</creationdate><title>Increase in bcl-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbil hippocampus</title><author>Shimazaki, Kuniko ; Ishida, Akihiro ; Kawai, Nobufumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-b255194c453f0833794c2ef6a5c9121fe381fc52bf9d837522508c37e111ee0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Apoptosis</topic><topic>bcl-2</topic><topic>Cell Death</topic><topic>Delayed neuronal death</topic><topic>Gerbil</topic><topic>Gerbillinae</topic><topic>Hippocampus</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - pathology</topic><topic>Ischemia</topic><topic>Ischemic Attack, Transient - pathology</topic><topic>Neurons - pathology</topic><topic>Oncoprotein</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Proto-Oncogene Proteins c-bcl-2</topic><topic>Staining and Labeling</topic><topic>Time Factors</topic><topic>Tolerance for neuronal death</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimazaki, Kuniko</creatorcontrib><creatorcontrib>Ishida, Akihiro</creatorcontrib><creatorcontrib>Kawai, Nobufumi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimazaki, Kuniko</au><au>Ishida, Akihiro</au><au>Kawai, Nobufumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increase in bcl-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbil hippocampus</atitle><jtitle>Neuroscience research</jtitle><addtitle>Neurosci Res</addtitle><date>1994-07-01</date><risdate>1994</risdate><volume>20</volume><issue>1</issue><spage>95</spage><epage>99</epage><pages>95-99</pages><issn>0168-0102</issn><eissn>1872-8111</eissn><abstract>We studied the expression of bcl-2 oncoprotein in the gerbil hippocampus after transient ischemia. Immunostaining using monoclonal antibody raised against bcl-2 oncoprotein revealed intense immunoreactivity in the CA1 area following 2 min of ischemia, which induced tolerance to subsequent ischemia and prevented delayed neuronal death (DND). Following ischemia for 5 min, however, bcl-2 oncoprotein immunoreactivity was decreased, reflecting neuronal death in the CA1 area. However, pretreatment with ischemia of 2 min that prevented DND due to subsequent ischemia for 5 min, showed increased immunoreactivity. On the other hand, following 1 min of ischemia which failed to induce tolerance, no increase in the bcl-2 oncoprotein was observed. The results evidenced that expression of bcl-2 oncoprotein in the CA1 area following brief ischemia is closely related to the acquisition of resistance to DND.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>7527133</pmid><doi>10.1016/0168-0102(94)90026-4</doi><tpages>5</tpages></addata></record> |
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subjects | Animals Antibodies, Monoclonal Apoptosis bcl-2 Cell Death Delayed neuronal death Gerbil Gerbillinae Hippocampus Hippocampus - metabolism Hippocampus - pathology Ischemia Ischemic Attack, Transient - pathology Neurons - pathology Oncoprotein Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-bcl-2 Staining and Labeling Time Factors Tolerance for neuronal death |
title | Increase in bcl-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbil hippocampus |
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