A new precipitation method with magnetic separation for high-density-lipoprotein cholesterol assay
We describe a new precipitation method for high-density-lipoprotein cholesterol quantitation. The new method uses magnetic force instead of centrifugal force to separate highdensity-lipoprotein from other lipoproteins that are fractioned with a precipitating reagent. The reagents used for the new me...
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| Veröffentlicht in: | Clinica chimica acta 1994-08, Vol.228 (2), p.133-142 |
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| container_title | Clinica chimica acta |
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| creator | Iida, Shinji Osawa, Susumu Yonemitsu, Hiroshi |
| description | We describe a new precipitation method for high-density-lipoprotein cholesterol quantitation. The new method uses magnetic force instead of centrifugal force to separate highdensity-lipoprotein from other lipoproteins that are fractioned with a precipitating reagent. The reagents used for the new method are the same as those for the conventional method except that magnetizable particles are included in the former. The magnetizable particles are used without any modifications. The correlation between the new and the centrifuge methods with dextran sulfate-magnesium chloride, sodium phosphotungstate-magnesium chloride and polyethylene glycol 6,000 were satisfactory (
r = 0.990, 0.997 and 0.997, respectively). The new method, which can be combined with any precipitating reagents used in conventional methods, is very simple to perform and does not need any special equipment. |
| doi_str_mv | 10.1016/0009-8981(94)90284-4 |
| format | Article |
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r = 0.990, 0.997 and 0.997, respectively). The new method, which can be combined with any precipitating reagents used in conventional methods, is very simple to perform and does not need any special equipment.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chemical Precipitation</subject><subject>Cholesterol, HDL - blood</subject><subject>Dextran Sulfate</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>High-density-lipoprotein</subject><subject>Humans</subject><subject>Indicators and Reagents</subject><subject>Lipoproteins, myelin</subject><subject>Magnesium Chloride</subject><subject>Magnetics</subject><subject>Magnetizable particles</subject><subject>Microspheres</subject><subject>Non-centrifugal separation</subject><subject>Phosphotungstic Acid</subject><subject>Polyethylene Glycols</subject><subject>Precipitation method</subject><subject>Proteins</subject><subject>Sensitivity and Specificity</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFLHDEYhoO06Gr7DxRykGIPY5NJnCSXgkhbBaEXew5J5osTmZmMSbay_75Zd9ljTyG8z_fx8nwInVNyTQntvhFCVCOVpFeKf1WklbzhR2hFpWAN46r9gFYH5ASd5vxSv5x09BgdCyUlYWSF7C2e4Q0vCVxYQjElxBlPUIbY47dQBjyZ5xlKcDjDYtIu9zHhITwPTQ9zDmXTjGGJS4oFwozdEEfIBVIcscnZbD6hj96MGT7v3zP05-ePp7v75vH3r4e728fGMdmVxlgrmbK9s52_6TsBTnAiufWkJ7aVwJRXTgkmvAJmlDPG3nBuO0md545Qdoa-7PbWJq_rWkFPITsYRzNDXGctOtnJlogK8h3oUsw5gddLCpNJG02J3qrVW296600rrt_Val7HLvb713aC_jC0d1nzy31usjOjT2Z2IR8w3jKhhKrY9x0G1cXfAElnF2B20Id6hKL7GP7f4x8Zdpel</recordid><startdate>19940801</startdate><enddate>19940801</enddate><creator>Iida, Shinji</creator><creator>Osawa, Susumu</creator><creator>Yonemitsu, Hiroshi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940801</creationdate><title>A new precipitation method with magnetic separation for high-density-lipoprotein cholesterol assay</title><author>Iida, Shinji ; Osawa, Susumu ; Yonemitsu, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-abb839bdcb6f5d67ec74084bf0d0b28e39f9c9737f9e3a9caab544b681cf4c013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chemical Precipitation</topic><topic>Cholesterol, HDL - blood</topic><topic>Dextran Sulfate</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>High-density-lipoprotein</topic><topic>Humans</topic><topic>Indicators and Reagents</topic><topic>Lipoproteins, myelin</topic><topic>Magnesium Chloride</topic><topic>Magnetics</topic><topic>Magnetizable particles</topic><topic>Microspheres</topic><topic>Non-centrifugal separation</topic><topic>Phosphotungstic Acid</topic><topic>Polyethylene Glycols</topic><topic>Precipitation method</topic><topic>Proteins</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iida, Shinji</creatorcontrib><creatorcontrib>Osawa, Susumu</creatorcontrib><creatorcontrib>Yonemitsu, Hiroshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iida, Shinji</au><au>Osawa, Susumu</au><au>Yonemitsu, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new precipitation method with magnetic separation for high-density-lipoprotein cholesterol assay</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1994-08-01</date><risdate>1994</risdate><volume>228</volume><issue>2</issue><spage>133</spage><epage>142</epage><pages>133-142</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>We describe a new precipitation method for high-density-lipoprotein cholesterol quantitation. The new method uses magnetic force instead of centrifugal force to separate highdensity-lipoprotein from other lipoproteins that are fractioned with a precipitating reagent. The reagents used for the new method are the same as those for the conventional method except that magnetizable particles are included in the former. The magnetizable particles are used without any modifications. The correlation between the new and the centrifuge methods with dextran sulfate-magnesium chloride, sodium phosphotungstate-magnesium chloride and polyethylene glycol 6,000 were satisfactory (
r = 0.990, 0.997 and 0.997, respectively). The new method, which can be combined with any precipitating reagents used in conventional methods, is very simple to perform and does not need any special equipment.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>7988030</pmid><doi>10.1016/0009-8981(94)90284-4</doi><tpages>10</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1994-08, Vol.228 (2), p.133-142 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Chemical Precipitation Cholesterol, HDL - blood Dextran Sulfate Fundamental and applied biological sciences. Psychology High-density-lipoprotein Humans Indicators and Reagents Lipoproteins, myelin Magnesium Chloride Magnetics Magnetizable particles Microspheres Non-centrifugal separation Phosphotungstic Acid Polyethylene Glycols Precipitation method Proteins Sensitivity and Specificity |
| title | A new precipitation method with magnetic separation for high-density-lipoprotein cholesterol assay |
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