Increased sensitivity of diagnostic latex agglutination tests in an ultrasonic standing wave field
A technique is described which increases the sensitivity of latex agglutination tests for soluble and particulate antigens. The levels of detection of tests for C-reative protein and E. coli 0157 respectively have been improved by ×256 and ×1024 compared with the standard test procedure of sample ro...
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| Veröffentlicht in: | Journal of immunological methods 1994-12, Vol.176 (2), p.169-177 |
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| container_end_page | 177 |
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| container_issue | 2 |
| container_start_page | 169 |
| container_title | Journal of immunological methods |
| container_volume | 176 |
| creator | Grundy, M.A. Moore, K. Coakley, W.T. |
| description | A technique is described which increases the sensitivity of latex agglutination tests for soluble and particulate antigens. The levels of detection of tests for C-reative protein and
E. coli 0157 respectively have been improved by ×256 and ×1024 compared with the standard test procedure of sample rotation on a test-card. This new method combines dilution of the test latex particles, a 2 min sample treatment in the ultrasonic standing wave field of a tubular piezo-electric transducer and subsequent examination by video-microscopy. Ultrasonic treatment is required to achieve increased localised concentrations of the latex particles in the standing wave field, and dilution of the latex is a critical requirement to allow agglutination to occur at low antigen concentrations. |
| doi_str_mv | 10.1016/0022-1759(94)90311-5 |
| format | Article |
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E. coli 0157 respectively have been improved by ×256 and ×1024 compared with the standard test procedure of sample rotation on a test-card. This new method combines dilution of the test latex particles, a 2 min sample treatment in the ultrasonic standing wave field of a tubular piezo-electric transducer and subsequent examination by video-microscopy. Ultrasonic treatment is required to achieve increased localised concentrations of the latex particles in the standing wave field, and dilution of the latex is a critical requirement to allow agglutination to occur at low antigen concentrations.</description><subject>Antigens, Bacterial - analysis</subject><subject>Biological and medical sciences</subject><subject>C-reactive protein</subject><subject>C-Reactive Protein - analysis</subject><subject>Escherichia coli - immunology</subject><subject>Escherichia coli 0157</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>High sensitivity agglutination test</subject><subject>Latex agglutination test</subject><subject>Latex Fixation Tests - methods</subject><subject>Microscopy - methods</subject><subject>Molecular immunology</subject><subject>Sensitivity and Specificity</subject><subject>Techniques</subject><subject>Tubular transducer</subject><subject>Ultrasonic standing wave field</subject><subject>Ultrasonics</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpSDcf_6AFHUpJD04kW7LsS6CEtAkEcknOYlYaLypeOdXI2-TfR9td9tjTHN5nhncexj5LcSmFbK-EqOtKGt1f9Op7LxopK_2BLWRn6sr0Qn9kiwPyiZ0Q_RZCSNGKY3Zs-q5pTLdgy_voEgKh54SRQg6bkN_4NHAfYBUnysHxETK-clitxjmHCDlMkWekTDxEDpHPY05AUywoZYg-xBX_CxvkQ8DRn7GjAUbC8_08Zc8_b59u7qqHx1_3Nz8eKqd0nSvpO41gFPaAWjo31ChAD1orr7EWsm87pdqlabQ3fim7pgWhG9056aVxSjSn7Nvu7kua_sylnl0HcjiOEHGayZq2a42qZQHVDnRpIko42JcU1pDerBR2q9ZuvdmtN9sr-0-t1WXty_7-vFyjPyztXZb86z4HcjAOCaILdMCaApVnCna9w7C42ARMllzA6NCHhC5bP4X_93gHZ2yV3g</recordid><startdate>19941202</startdate><enddate>19941202</enddate><creator>Grundy, M.A.</creator><creator>Moore, K.</creator><creator>Coakley, W.T.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941202</creationdate><title>Increased sensitivity of diagnostic latex agglutination tests in an ultrasonic standing wave field</title><author>Grundy, M.A. ; Moore, K. ; Coakley, W.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-1d85ea74e9ae51ccf2e0a5f554d5e201968446b735d7db1836a05358c1d17c403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antigens, Bacterial - analysis</topic><topic>Biological and medical sciences</topic><topic>C-reactive protein</topic><topic>C-Reactive Protein - analysis</topic><topic>Escherichia coli - immunology</topic><topic>Escherichia coli 0157</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>High sensitivity agglutination test</topic><topic>Latex agglutination test</topic><topic>Latex Fixation Tests - methods</topic><topic>Microscopy - methods</topic><topic>Molecular immunology</topic><topic>Sensitivity and Specificity</topic><topic>Techniques</topic><topic>Tubular transducer</topic><topic>Ultrasonic standing wave field</topic><topic>Ultrasonics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grundy, M.A.</creatorcontrib><creatorcontrib>Moore, K.</creatorcontrib><creatorcontrib>Coakley, W.T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grundy, M.A.</au><au>Moore, K.</au><au>Coakley, W.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased sensitivity of diagnostic latex agglutination tests in an ultrasonic standing wave field</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1994-12-02</date><risdate>1994</risdate><volume>176</volume><issue>2</issue><spage>169</spage><epage>177</epage><pages>169-177</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>A technique is described which increases the sensitivity of latex agglutination tests for soluble and particulate antigens. The levels of detection of tests for C-reative protein and
E. coli 0157 respectively have been improved by ×256 and ×1024 compared with the standard test procedure of sample rotation on a test-card. This new method combines dilution of the test latex particles, a 2 min sample treatment in the ultrasonic standing wave field of a tubular piezo-electric transducer and subsequent examination by video-microscopy. Ultrasonic treatment is required to achieve increased localised concentrations of the latex particles in the standing wave field, and dilution of the latex is a critical requirement to allow agglutination to occur at low antigen concentrations.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>7983378</pmid><doi>10.1016/0022-1759(94)90311-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of immunological methods, 1994-12, Vol.176 (2), p.169-177 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antigens, Bacterial - analysis Biological and medical sciences C-reactive protein C-Reactive Protein - analysis Escherichia coli - immunology Escherichia coli 0157 Fundamental and applied biological sciences. Psychology Fundamental immunology High sensitivity agglutination test Latex agglutination test Latex Fixation Tests - methods Microscopy - methods Molecular immunology Sensitivity and Specificity Techniques Tubular transducer Ultrasonic standing wave field Ultrasonics |
| title | Increased sensitivity of diagnostic latex agglutination tests in an ultrasonic standing wave field |
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