The production and characterization of a new monoclonal antibody to the trefoil peptide human spasmolytic polypeptide
Human spasmolytic polypeptide (hSP) is a member of the growing family of trefoil peptides which are expressed in discrete regions of the body, most notably the gastrointestinal tract. Much of the research into the localization of the spasmolytic polypeptide has relied on hybridization in situ to det...
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| Veröffentlicht in: | The Histochemical journal 1994-08, Vol.26 (8), p.644-647 |
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| container_title | The Histochemical journal |
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| creator | ELIA, G CHINERY, R HANBY, A. M POULSOM, R WRIGHT, N. A |
| description | Human spasmolytic polypeptide (hSP) is a member of the growing family of trefoil peptides which are expressed in discrete regions of the body, most notably the gastrointestinal tract. Much of the research into the localization of the spasmolytic polypeptide has relied on hybridization in situ to detect its mRNA, due to the absence of a suitable antibody. The aim of the present study was to develop and characterize a monoclonal antibody against the human spasmolytic polypeptide, using a combination of immunohistochemistry and hybridization in situ. After immunoblotting, the antibody detected a 14 kDa protein in gastrointestinal tissue extracts from the stomach and small intestine only. Using immunohistochemistry, human spasmolytic polypeptide showed a distinctive staining pattern in the duodenum which co-localized with its mRNA. The co-localization of the immunoreactive peptide with its mRNA provides good evidence that the antibody truly recognized human spasmolytic polypeptide. |
| doi_str_mv | 10.1007/bf00158289 |
| format | Article |
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The co-localization of the immunoreactive peptide with its mRNA provides good evidence that the antibody truly recognized human spasmolytic polypeptide.</description><identifier>ISSN: 0018-2214</identifier><identifier>EISSN: 1573-6865</identifier><identifier>DOI: 10.1007/bf00158289</identifier><identifier>PMID: 7982789</identifier><identifier>CODEN: HISJAE</identifier><language>eng</language><publisher>London: Kluwer</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Monoclonal - metabolism ; Antibody Specificity ; Biological and medical sciences ; Blotting, Western ; Female ; Fundamental and applied biological sciences. Psychology ; General aspects ; Growth Substances - genetics ; Growth Substances - immunology ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Mice ; Mice, Inbred BALB C ; Mucins ; Muscle Proteins ; Neuropeptides ; Peptides - genetics ; Peptides - immunology ; Rats ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Trefoil Factor-2 ; Trefoil Factor-3 ; Vertebrates: digestive system</subject><ispartof>The Histochemical journal, 1994-08, Vol.26 (8), p.644-647</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-4ab96dc9699144c46ddca1cb2d93e0dc9e526deb24434d78154b1e9310f6864d3</citedby><cites>FETCH-LOGICAL-c377t-4ab96dc9699144c46ddca1cb2d93e0dc9e526deb24434d78154b1e9310f6864d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4215209$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7982789$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ELIA, G</creatorcontrib><creatorcontrib>CHINERY, R</creatorcontrib><creatorcontrib>HANBY, A. M</creatorcontrib><creatorcontrib>POULSOM, R</creatorcontrib><creatorcontrib>WRIGHT, N. A</creatorcontrib><title>The production and characterization of a new monoclonal antibody to the trefoil peptide human spasmolytic polypeptide</title><title>The Histochemical journal</title><addtitle>Histochem J</addtitle><description>Human spasmolytic polypeptide (hSP) is a member of the growing family of trefoil peptides which are expressed in discrete regions of the body, most notably the gastrointestinal tract. Much of the research into the localization of the spasmolytic polypeptide has relied on hybridization in situ to detect its mRNA, due to the absence of a suitable antibody. The aim of the present study was to develop and characterize a monoclonal antibody against the human spasmolytic polypeptide, using a combination of immunohistochemistry and hybridization in situ. After immunoblotting, the antibody detected a 14 kDa protein in gastrointestinal tissue extracts from the stomach and small intestine only. Using immunohistochemistry, human spasmolytic polypeptide showed a distinctive staining pattern in the duodenum which co-localized with its mRNA. The co-localization of the immunoreactive peptide with its mRNA provides good evidence that the antibody truly recognized human spasmolytic polypeptide.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Growth Substances - genetics</subject><subject>Growth Substances - immunology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mucins</subject><subject>Muscle Proteins</subject><subject>Neuropeptides</subject><subject>Peptides - genetics</subject><subject>Peptides - immunology</subject><subject>Rats</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Trefoil Factor-2</subject><subject>Trefoil Factor-3</subject><subject>Vertebrates: digestive system</subject><issn>0018-2214</issn><issn>1573-6865</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEFLwzAAhYMoc04v3oUcxINQTdK0aY46nAoDL_Nc0iRlkTSpTYrMX290dacH7308eA-AS4zuMELsvmkRwkVFKn4E5rhgeVZWZXEM5smuMkIwPQVnIXwghDhj5QzMGK8Iq_gcjJuthv3g1Sij8Q4Kp6DcikHIqAfzLf5M30IBnf6CnXdeWu-ETWA0jVc7GD2MqSMOuvXGwl730SgNt2MnHAy9CJ23u2gk7JNO6Tk4aYUN-mLSBXhfPW2WL9n67fl1-bDOZM5YzKhoeKkkLznHlEpaKiUFlg1RPNcoBbogpdINoTSnilW4oA3WPMeoTQdQlS_Azb43LfwcdYh1Z4LU1gqn_RhqljDMK57A2z0oBx9CmlL3g-nEsKsxqn8_rh9X_x8n-GpqHZtOqwM6nZry6ykXQQrbDsJJEw4YJbggiOc_zPKFzg</recordid><startdate>19940801</startdate><enddate>19940801</enddate><creator>ELIA, G</creator><creator>CHINERY, R</creator><creator>HANBY, A. 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A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-4ab96dc9699144c46ddca1cb2d93e0dc9e526deb24434d78154b1e9310f6864d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Monoclonal - metabolism</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The production and characterization of a new monoclonal antibody to the trefoil peptide human spasmolytic polypeptide</atitle><jtitle>The Histochemical journal</jtitle><addtitle>Histochem J</addtitle><date>1994-08-01</date><risdate>1994</risdate><volume>26</volume><issue>8</issue><spage>644</spage><epage>647</epage><pages>644-647</pages><issn>0018-2214</issn><eissn>1573-6865</eissn><coden>HISJAE</coden><abstract>Human spasmolytic polypeptide (hSP) is a member of the growing family of trefoil peptides which are expressed in discrete regions of the body, most notably the gastrointestinal tract. Much of the research into the localization of the spasmolytic polypeptide has relied on hybridization in situ to detect its mRNA, due to the absence of a suitable antibody. The aim of the present study was to develop and characterize a monoclonal antibody against the human spasmolytic polypeptide, using a combination of immunohistochemistry and hybridization in situ. After immunoblotting, the antibody detected a 14 kDa protein in gastrointestinal tissue extracts from the stomach and small intestine only. Using immunohistochemistry, human spasmolytic polypeptide showed a distinctive staining pattern in the duodenum which co-localized with its mRNA. The co-localization of the immunoreactive peptide with its mRNA provides good evidence that the antibody truly recognized human spasmolytic polypeptide.</abstract><cop>London</cop><pub>Kluwer</pub><pmid>7982789</pmid><doi>10.1007/bf00158289</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0018-2214 |
| ispartof | The Histochemical journal, 1994-08, Vol.26 (8), p.644-647 |
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| language | eng |
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| subjects | Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - metabolism Antibody Specificity Biological and medical sciences Blotting, Western Female Fundamental and applied biological sciences. Psychology General aspects Growth Substances - genetics Growth Substances - immunology Humans Immunohistochemistry In Situ Hybridization Mice Mice, Inbred BALB C Mucins Muscle Proteins Neuropeptides Peptides - genetics Peptides - immunology Rats RNA, Messenger - analysis RNA, Messenger - genetics Trefoil Factor-2 Trefoil Factor-3 Vertebrates: digestive system |
| title | The production and characterization of a new monoclonal antibody to the trefoil peptide human spasmolytic polypeptide |
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