Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence Application to homogeneous digoxin immunoassay
A laser-induced fluorescence (LIF) immunoassay technique based on capillary electrophoretic (CE) separation is demonstrated. The analysis of digoxin in serum at clinically useful concentration levels of 10 −9 to 10 −10 M is achieved using this technique. The chemistry presented here using digoxigeni...
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| Veröffentlicht in: | Journal of Chromatography A 1994-10, Vol.680 (2), p.425-430 |
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| container_end_page | 430 |
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| container_title | Journal of Chromatography A |
| container_volume | 680 |
| creator | Chen, Fu-Tai A. Pentoney, Stephen L. |
| description | A laser-induced fluorescence (LIF) immunoassay technique based on capillary electrophoretic (CE) separation is demonstrated. The analysis of digoxin in serum at clinically useful concentration levels of 10
−9 to 10
−10
M is achieved using this technique. The chemistry presented here using digoxigenin-labeled B-phycoerythrin was selected as a convenient model for the exploration of CE-LIF-based immunoassays. The LIF system described here exhibits detection limits in the low 10
−11
M range for several common fluorophores. The data presented in this report are one of the first examples of nanomolar quantitative analysis in a human serum matrix by CE. |
| doi_str_mv | 10.1016/0021-9673(94)85139-5 |
| format | Article |
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−9 to 10
−10
M is achieved using this technique. The chemistry presented here using digoxigenin-labeled B-phycoerythrin was selected as a convenient model for the exploration of CE-LIF-based immunoassays. The LIF system described here exhibits detection limits in the low 10
−11
M range for several common fluorophores. The data presented in this report are one of the first examples of nanomolar quantitative analysis in a human serum matrix by CE.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/0021-9673(94)85139-5</identifier><identifier>PMID: 7981824</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Binding, Competitive ; Capillary Action ; Digoxigenin ; Digoxin - blood ; Electrophoresis - methods ; Fluorescence ; Fluorescent Dyes ; Humans ; Immunoassay ; Lasers ; Phycoerythrin - chemistry ; Phycoerythrin - isolation & purification</subject><ispartof>Journal of Chromatography A, 1994-10, Vol.680 (2), p.425-430</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-2305d0b9dcda5bb1d3b183cc8af1610cdf2881f7dfc7bb82e4d79a3e5fab2dbb3</citedby><cites>FETCH-LOGICAL-c357t-2305d0b9dcda5bb1d3b183cc8af1610cdf2881f7dfc7bb82e4d79a3e5fab2dbb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0021967394851395$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7981824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Fu-Tai A.</creatorcontrib><creatorcontrib>Pentoney, Stephen L.</creatorcontrib><title>Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence Application to homogeneous digoxin immunoassay</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A laser-induced fluorescence (LIF) immunoassay technique based on capillary electrophoretic (CE) separation is demonstrated. The analysis of digoxin in serum at clinically useful concentration levels of 10
−9 to 10
−10
M is achieved using this technique. The chemistry presented here using digoxigenin-labeled B-phycoerythrin was selected as a convenient model for the exploration of CE-LIF-based immunoassays. The LIF system described here exhibits detection limits in the low 10
−11
M range for several common fluorophores. The data presented in this report are one of the first examples of nanomolar quantitative analysis in a human serum matrix by CE.</description><subject>Binding, Competitive</subject><subject>Capillary Action</subject><subject>Digoxigenin</subject><subject>Digoxin - blood</subject><subject>Electrophoresis - methods</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Lasers</subject><subject>Phycoerythrin - chemistry</subject><subject>Phycoerythrin - isolation & purification</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu1TAU9AJUSuEPQPIKwSJg52Vng1SueFSqxKZdW34cNwclcbATIP0dfhSn96pLVpY8M2fOmSHkFWfvOePtB8ZKXnStqN529TvZ8Kormifk_PH7GXme0g_GuGCiPCNnopNclvU5-XvoddR2gYj3esEw0eCpw7vwB-9gwqkYtIEBHP1UzP1mA8Rt6SNO1GzU6hmHQceNZoZdYpj7ECFhor9x6emgE8QCJ7farPfDuoMWJgv0cp4HtEe_JdA-jCG7QVjTyXuiOI7rFHRKentBnno9JHh5ei_I7ZfPN4dvxfX3r1eHy-vCVo1YirJijWOmc9bpxhjuKsNlZa3UnrecWedLKbkXzlthjCyhdqLTFTRem9IZU12QN8e5cww_V0iLGjEvnE98WE2JVra8rHkm1keijSGlCF7NEccchOJM7X2oPXi1B6-6Wj30oZose32av5oR3KPoVEbGPx5xyEf-QogqWdzzchhzvsoF_L_BP7Pxo3o</recordid><startdate>19941007</startdate><enddate>19941007</enddate><creator>Chen, Fu-Tai A.</creator><creator>Pentoney, Stephen L.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941007</creationdate><title>Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence Application to homogeneous digoxin immunoassay</title><author>Chen, Fu-Tai A. ; Pentoney, Stephen L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-2305d0b9dcda5bb1d3b183cc8af1610cdf2881f7dfc7bb82e4d79a3e5fab2dbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Binding, Competitive</topic><topic>Capillary Action</topic><topic>Digoxigenin</topic><topic>Digoxin - blood</topic><topic>Electrophoresis - methods</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Lasers</topic><topic>Phycoerythrin - chemistry</topic><topic>Phycoerythrin - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Fu-Tai A.</creatorcontrib><creatorcontrib>Pentoney, Stephen L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Fu-Tai A.</au><au>Pentoney, Stephen L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence Application to homogeneous digoxin immunoassay</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1994-10-07</date><risdate>1994</risdate><volume>680</volume><issue>2</issue><spage>425</spage><epage>430</epage><pages>425-430</pages><issn>0021-9673</issn><abstract>A laser-induced fluorescence (LIF) immunoassay technique based on capillary electrophoretic (CE) separation is demonstrated. The analysis of digoxin in serum at clinically useful concentration levels of 10
−9 to 10
−10
M is achieved using this technique. The chemistry presented here using digoxigenin-labeled B-phycoerythrin was selected as a convenient model for the exploration of CE-LIF-based immunoassays. The LIF system described here exhibits detection limits in the low 10
−11
M range for several common fluorophores. The data presented in this report are one of the first examples of nanomolar quantitative analysis in a human serum matrix by CE.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7981824</pmid><doi>10.1016/0021-9673(94)85139-5</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of Chromatography A, 1994-10, Vol.680 (2), p.425-430 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Binding, Competitive Capillary Action Digoxigenin Digoxin - blood Electrophoresis - methods Fluorescence Fluorescent Dyes Humans Immunoassay Lasers Phycoerythrin - chemistry Phycoerythrin - isolation & purification |
| title | Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence Application to homogeneous digoxin immunoassay |
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