[35] In Situ cDNA;mRNA hybridization: Development of technique to measure mRNA levels in individual cells

In this article we have described our protocol for in situ cDNA:mRNA hybridization and a variety of methodological considerations we have entertained to optimize the procedure. It should be stressed that each different system will have its own special characteristics and require optimization to obta...

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Veröffentlicht in:	Methods in Enzymology 1986, Vol.124, p.510-533
Hauptverfasser:	Wilcox, Josiah N., Gee, Connie E., Roberts, James L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Autoradiography - methods Brain - metabolism DNA - metabolism Genes Nucleic Acid Hybridization Pituitary Gland - metabolism Pro-Opiomelanocortin - genetics Rats RNA, Messenger - analysis RNA, Messenger - genetics Transcription, Genetic Tritium
Online-Zugang:	Volltext
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