Suppression of Cyclin D1 mRNA Expression by the Angiogenesis Inhibitor TNP-470 (AGM-1470) in Vascular Endothelial Cells
The effects of TNP-470 on DNA synthesis and the expression of c-myc and cyclin D1 mRNAs were investigated in human umbilical vein endothelial (HUVE) cells synchronized by serum depletion and stimulated with bFGF and serum. DNA synthesis occurring 16 h after stimulation was inhibited when TNP-470 was...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-11, Vol.204 (3), p.1067-1073 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Hori, A. Ikeyama, S. Sudo, K. |
| description | The effects of TNP-470 on DNA synthesis and the expression of c-myc and cyclin D1 mRNAs were investigated in human umbilical vein endothelial (HUVE) cells synchronized by serum depletion and stimulated with bFGF and serum. DNA synthesis occurring 16 h after stimulation was inhibited when TNP-470 was present from 2 to 6 h after stimulation. C-myc mRNA expression occurring 2 h after stimulation was not inhibited by the addition of TNP-470. Cyclin D1 mRNA expression occurring 6 h after stimulation was suppressed in the presence of TNP-470 from 2 to 6 h after stimulation. On the other hand, cyclin D1 expression was not suppressed in the TNP-470-insensitive human tumor cell line WiDr. These results suggest that the inhibition of HUVE cells by TNP-470 is due to the suppression of cyclin D1 expression in mid G1 phase. |
| doi_str_mv | 10.1006/bbrc.1994.2571 |
| format | Article |
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DNA synthesis occurring 16 h after stimulation was inhibited when TNP-470 was present from 2 to 6 h after stimulation. C-myc mRNA expression occurring 2 h after stimulation was not inhibited by the addition of TNP-470. Cyclin D1 mRNA expression occurring 6 h after stimulation was suppressed in the presence of TNP-470 from 2 to 6 h after stimulation. On the other hand, cyclin D1 expression was not suppressed in the TNP-470-insensitive human tumor cell line WiDr. These results suggest that the inhibition of HUVE cells by TNP-470 is due to the suppression of cyclin D1 expression in mid G1 phase.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1994.2571</identifier><identifier>PMID: 7980579</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antibiotics, Antineoplastic - pharmacology ; Base Sequence ; Cells, Cultured ; Culture Media, Serum-Free ; Cyclin D1 ; Cyclins - biosynthesis ; Cyclohexanes ; DNA Primers ; DNA Replication - drug effects ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Fibroblast Growth Factor 2 - pharmacology ; Gene Expression - drug effects ; Genes, myc - drug effects ; Humans ; Kinetics ; Molecular Sequence Data ; Oligonucleotide Probes ; Oncogene Proteins - biosynthesis ; Polymerase Chain Reaction ; Proto-Oncogene Proteins c-myc - biosynthesis ; RNA, Messenger - biosynthesis ; Sesquiterpenes - pharmacology ; Suppression, Genetic ; Time Factors ; Umbilical Veins</subject><ispartof>Biochemical and biophysical research communications, 1994-11, Vol.204 (3), p.1067-1073</ispartof><rights>1994 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c320t-11aad7572d709aa6ad6b2724ace336808028ce98a0ae1866043635c5d9fdeef83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1994.2571$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7980579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hori, A.</creatorcontrib><creatorcontrib>Ikeyama, S.</creatorcontrib><creatorcontrib>Sudo, K.</creatorcontrib><title>Suppression of Cyclin D1 mRNA Expression by the Angiogenesis Inhibitor TNP-470 (AGM-1470) in Vascular Endothelial Cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The effects of TNP-470 on DNA synthesis and the expression of c-myc and cyclin D1 mRNAs were investigated in human umbilical vein endothelial (HUVE) cells synchronized by serum depletion and stimulated with bFGF and serum. DNA synthesis occurring 16 h after stimulation was inhibited when TNP-470 was present from 2 to 6 h after stimulation. C-myc mRNA expression occurring 2 h after stimulation was not inhibited by the addition of TNP-470. Cyclin D1 mRNA expression occurring 6 h after stimulation was suppressed in the presence of TNP-470 from 2 to 6 h after stimulation. On the other hand, cyclin D1 expression was not suppressed in the TNP-470-insensitive human tumor cell line WiDr. These results suggest that the inhibition of HUVE cells by TNP-470 is due to the suppression of cyclin D1 expression in mid G1 phase.</description><subject>Antibiotics, Antineoplastic - pharmacology</subject><subject>Base Sequence</subject><subject>Cells, Cultured</subject><subject>Culture Media, Serum-Free</subject><subject>Cyclin D1</subject><subject>Cyclins - biosynthesis</subject><subject>Cyclohexanes</subject><subject>DNA Primers</subject><subject>DNA Replication - drug effects</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Gene Expression - drug effects</subject><subject>Genes, myc - drug effects</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotide Probes</subject><subject>Oncogene Proteins - biosynthesis</subject><subject>Polymerase Chain Reaction</subject><subject>Proto-Oncogene Proteins c-myc - biosynthesis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Sesquiterpenes - pharmacology</subject><subject>Suppression, Genetic</subject><subject>Time Factors</subject><subject>Umbilical Veins</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1vEzEQhi0EKqFw5YbkE4LDhrF31x_HKIRSqRQEBXGzvPZsa7RZp_YukH-Po0S9cZqR3g_NPIS8ZLBkAOJd1yW3ZFo3S95K9ogsGGioOIPmMVlAcVRcs59PybOcfwEw1gh9Rs6kVtBKvSB_vs27XcKcQxxp7Ol674Yw0veMbr9er-jm74PY7el0h3Q13oZ4iyPmkOnleBe6MMVEb66_VI0E-mZ18aliZXtLS80Pm9082EQ3o48lPQQ70DUOQ35OnvR2yPjiNM_J9w-bm_XH6urzxeV6dVW5msNUMWatl63kXoK2VlgvOi55Yx3WtVCggCuHWlmwyJQQ0NSibl3rde8Re1Wfk9fH3l2K9zPmyWxDduUCO2Kcs5FCFWyCF-PyaHQp5pywN7sUtjbtDQNzIG0OpM2BtDmQLoFXp-a526J_sJ_QFl0ddSzv_Q6YTHYBR4c-JHST8TH8r_ofM8aLJQ</recordid><startdate>19941115</startdate><enddate>19941115</enddate><creator>Hori, A.</creator><creator>Ikeyama, S.</creator><creator>Sudo, K.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941115</creationdate><title>Suppression of Cyclin D1 mRNA Expression by the Angiogenesis Inhibitor TNP-470 (AGM-1470) in Vascular Endothelial Cells</title><author>Hori, A. ; Ikeyama, S. ; Sudo, K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-11aad7572d709aa6ad6b2724ace336808028ce98a0ae1866043635c5d9fdeef83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antibiotics, Antineoplastic - pharmacology</topic><topic>Base Sequence</topic><topic>Cells, Cultured</topic><topic>Culture Media, Serum-Free</topic><topic>Cyclin D1</topic><topic>Cyclins - biosynthesis</topic><topic>Cyclohexanes</topic><topic>DNA Primers</topic><topic>DNA Replication - drug effects</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Gene Expression - drug effects</topic><topic>Genes, myc - drug effects</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotide Probes</topic><topic>Oncogene Proteins - biosynthesis</topic><topic>Polymerase Chain Reaction</topic><topic>Proto-Oncogene Proteins c-myc - biosynthesis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Sesquiterpenes - pharmacology</topic><topic>Suppression, Genetic</topic><topic>Time Factors</topic><topic>Umbilical Veins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hori, A.</creatorcontrib><creatorcontrib>Ikeyama, S.</creatorcontrib><creatorcontrib>Sudo, K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hori, A.</au><au>Ikeyama, S.</au><au>Sudo, K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of Cyclin D1 mRNA Expression by the Angiogenesis Inhibitor TNP-470 (AGM-1470) in Vascular Endothelial Cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-11-15</date><risdate>1994</risdate><volume>204</volume><issue>3</issue><spage>1067</spage><epage>1073</epage><pages>1067-1073</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The effects of TNP-470 on DNA synthesis and the expression of c-myc and cyclin D1 mRNAs were investigated in human umbilical vein endothelial (HUVE) cells synchronized by serum depletion and stimulated with bFGF and serum. DNA synthesis occurring 16 h after stimulation was inhibited when TNP-470 was present from 2 to 6 h after stimulation. C-myc mRNA expression occurring 2 h after stimulation was not inhibited by the addition of TNP-470. Cyclin D1 mRNA expression occurring 6 h after stimulation was suppressed in the presence of TNP-470 from 2 to 6 h after stimulation. On the other hand, cyclin D1 expression was not suppressed in the TNP-470-insensitive human tumor cell line WiDr. These results suggest that the inhibition of HUVE cells by TNP-470 is due to the suppression of cyclin D1 expression in mid G1 phase.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7980579</pmid><doi>10.1006/bbrc.1994.2571</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1994-11, Vol.204 (3), p.1067-1073 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antibiotics, Antineoplastic - pharmacology Base Sequence Cells, Cultured Culture Media, Serum-Free Cyclin D1 Cyclins - biosynthesis Cyclohexanes DNA Primers DNA Replication - drug effects Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Fibroblast Growth Factor 2 - pharmacology Gene Expression - drug effects Genes, myc - drug effects Humans Kinetics Molecular Sequence Data Oligonucleotide Probes Oncogene Proteins - biosynthesis Polymerase Chain Reaction Proto-Oncogene Proteins c-myc - biosynthesis RNA, Messenger - biosynthesis Sesquiterpenes - pharmacology Suppression, Genetic Time Factors Umbilical Veins |
| title | Suppression of Cyclin D1 mRNA Expression by the Angiogenesis Inhibitor TNP-470 (AGM-1470) in Vascular Endothelial Cells |
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