Detection and characterization of pangola stunt Fijivirus from Australia using cloned cDNA probes
Four cDNA clones were generated from the genomic dsRNA of an Australian isolate of pangola stunt Fijivirus (PaSV). Each clone hybridized with nucleic acid extracts from PaSV infected plants but not healthy plants. Further, each clone hybridized with more than one segment of the PaSV dsRNA genome. On...
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| Veröffentlicht in: | Archives of virology 1994-01, Vol.135 (3/4), p.397-404 |
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| container_end_page | 404 |
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| container_issue | 3/4 |
| container_start_page | 397 |
| container_title | Archives of virology |
| container_volume | 135 |
| creator | Karan, M Dale, J.L Bateson, M.F Harding, R.M Teakle, D.S |
| description | Four cDNA clones were generated from the genomic dsRNA of an Australian isolate of pangola stunt Fijivirus (PaSV). Each clone hybridized with nucleic acid extracts from PaSV infected plants but not healthy plants. Further, each clone hybridized with more than one segment of the PaSV dsRNA genome. One clone was used to demonstrate that homology existed between the Australian isolate of PaSV and a South American isolate of PaSV although the isolates differed in the sizes of the genomic dsRNAs and in the vector species. The clone also hybridized with some segments of the maize rough dwarf Fijivirus genome. |
| doi_str_mv | 10.1007/BF01310023 |
| format | Article |
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Each clone hybridized with nucleic acid extracts from PaSV infected plants but not healthy plants. Further, each clone hybridized with more than one segment of the PaSV dsRNA genome. One clone was used to demonstrate that homology existed between the Australian isolate of PaSV and a South American isolate of PaSV although the isolates differed in the sizes of the genomic dsRNAs and in the vector species. The clone also hybridized with some segments of the maize rough dwarf Fijivirus genome.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/BF01310023</identifier><identifier>PMID: 7979975</identifier><language>eng</language><publisher>Austria</publisher><subject>arboviruses ; Australia ; Autoradiography ; Cloning, Molecular ; Deoxycytosine Nucleotides - metabolism ; DNA Probes ; DNA, Complementary ; Genome, Viral ; maize rough dwarf virus ; pangola stunt fijivirus ; Pangola stunt virus ; Phosphorus Radioisotopes ; Poaceae - virology ; Reoviridae - genetics ; Reoviridae - isolation & purification ; RNA ; RNA, Double-Stranded - analysis ; RNA, Double-Stranded - biosynthesis ; RNA, Double-Stranded - genetics ; Sequence Homology, Nucleic Acid ; South America ; strain differences</subject><ispartof>Archives of virology, 1994-01, Vol.135 (3/4), p.397-404</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-201fe7c6b3c85ed1cf8456eb89327745ff494166744dc0cc6e4ac6082dce0913</citedby><cites>FETCH-LOGICAL-c337t-201fe7c6b3c85ed1cf8456eb89327745ff494166744dc0cc6e4ac6082dce0913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7979975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karan, M</creatorcontrib><creatorcontrib>Dale, J.L</creatorcontrib><creatorcontrib>Bateson, M.F</creatorcontrib><creatorcontrib>Harding, R.M</creatorcontrib><creatorcontrib>Teakle, D.S</creatorcontrib><title>Detection and characterization of pangola stunt Fijivirus from Australia using cloned cDNA probes</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>Four cDNA clones were generated from the genomic dsRNA of an Australian isolate of pangola stunt Fijivirus (PaSV). 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The clone also hybridized with some segments of the maize rough dwarf Fijivirus genome.</description><subject>arboviruses</subject><subject>Australia</subject><subject>Autoradiography</subject><subject>Cloning, Molecular</subject><subject>Deoxycytosine Nucleotides - metabolism</subject><subject>DNA Probes</subject><subject>DNA, Complementary</subject><subject>Genome, Viral</subject><subject>maize rough dwarf virus</subject><subject>pangola stunt fijivirus</subject><subject>Pangola stunt virus</subject><subject>Phosphorus Radioisotopes</subject><subject>Poaceae - virology</subject><subject>Reoviridae - genetics</subject><subject>Reoviridae - isolation & purification</subject><subject>RNA</subject><subject>RNA, Double-Stranded - analysis</subject><subject>RNA, Double-Stranded - biosynthesis</subject><subject>RNA, Double-Stranded - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>South America</subject><subject>strain differences</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDFPwzAQhS0EKqWwsCM8MSAF7Nix47G0FJAqGCiz5Th2cZXGxU6Q4NdjaAUj053ufXqn9wA4xegKI8Svb2YIk7TlZA8MMSV5VnJR7oMhIohmJUPlITiKcYVQOpBiAAZccCF4MQRqajqjO-dbqNoa6lcVlO5McJ_q5-gt3Kh26RsFY9e3HZy5lXt3oY_QBr-G4z52QTVOwT66dgl141uTfKaPY7gJvjLxGBxY1URzspsjsJjdLib32fzp7mEynmeaEN5lOcLWcM0qosvC1FjbkhbMVKUgOee0sJYKihnjlNYaac0MVToly2ttkMBkBC62tunrW29iJ9cuatM0qjW-j5KzsigEof-CmCNOCCkSeLkFdfAxBmPlJri1Ch8SI_ndu_zrPcFnO9e-Wpv6F90VnfTzrW6Vl2oZXJQvzykzQZgKznBOvgCB8IZp</recordid><startdate>19940101</startdate><enddate>19940101</enddate><creator>Karan, M</creator><creator>Dale, J.L</creator><creator>Bateson, M.F</creator><creator>Harding, R.M</creator><creator>Teakle, D.S</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940101</creationdate><title>Detection and characterization of pangola stunt Fijivirus from Australia using cloned cDNA probes</title><author>Karan, M ; Dale, J.L ; Bateson, M.F ; Harding, R.M ; Teakle, D.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-201fe7c6b3c85ed1cf8456eb89327745ff494166744dc0cc6e4ac6082dce0913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>arboviruses</topic><topic>Australia</topic><topic>Autoradiography</topic><topic>Cloning, Molecular</topic><topic>Deoxycytosine Nucleotides - metabolism</topic><topic>DNA Probes</topic><topic>DNA, Complementary</topic><topic>Genome, Viral</topic><topic>maize rough dwarf virus</topic><topic>pangola stunt fijivirus</topic><topic>Pangola stunt virus</topic><topic>Phosphorus Radioisotopes</topic><topic>Poaceae - virology</topic><topic>Reoviridae - genetics</topic><topic>Reoviridae - isolation & purification</topic><topic>RNA</topic><topic>RNA, Double-Stranded - analysis</topic><topic>RNA, Double-Stranded - biosynthesis</topic><topic>RNA, Double-Stranded - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>South America</topic><topic>strain differences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karan, M</creatorcontrib><creatorcontrib>Dale, J.L</creatorcontrib><creatorcontrib>Bateson, M.F</creatorcontrib><creatorcontrib>Harding, R.M</creatorcontrib><creatorcontrib>Teakle, D.S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karan, M</au><au>Dale, J.L</au><au>Bateson, M.F</au><au>Harding, R.M</au><au>Teakle, D.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and characterization of pangola stunt Fijivirus from Australia using cloned cDNA probes</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1994-01-01</date><risdate>1994</risdate><volume>135</volume><issue>3/4</issue><spage>397</spage><epage>404</epage><pages>397-404</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Four cDNA clones were generated from the genomic dsRNA of an Australian isolate of pangola stunt Fijivirus (PaSV). Each clone hybridized with nucleic acid extracts from PaSV infected plants but not healthy plants. Further, each clone hybridized with more than one segment of the PaSV dsRNA genome. One clone was used to demonstrate that homology existed between the Australian isolate of PaSV and a South American isolate of PaSV although the isolates differed in the sizes of the genomic dsRNAs and in the vector species. The clone also hybridized with some segments of the maize rough dwarf Fijivirus genome.</abstract><cop>Austria</cop><pmid>7979975</pmid><doi>10.1007/BF01310023</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-8608 |
| ispartof | Archives of virology, 1994-01, Vol.135 (3/4), p.397-404 |
| issn | 0304-8608 1432-8798 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76855934 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | arboviruses Australia Autoradiography Cloning, Molecular Deoxycytosine Nucleotides - metabolism DNA Probes DNA, Complementary Genome, Viral maize rough dwarf virus pangola stunt fijivirus Pangola stunt virus Phosphorus Radioisotopes Poaceae - virology Reoviridae - genetics Reoviridae - isolation & purification RNA RNA, Double-Stranded - analysis RNA, Double-Stranded - biosynthesis RNA, Double-Stranded - genetics Sequence Homology, Nucleic Acid South America strain differences |
| title | Detection and characterization of pangola stunt Fijivirus from Australia using cloned cDNA probes |
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