Effect of Periodate-Oxidized ATP and Other Nucleotides on Firefly Luciferase

Effect of Periodate-Oxidized ATP and Other Nucleotides on Firefly Luciferase

Addition of periodate-oxidized ATP (oATP) to firefly luciferase-containing reaction mixtures enhanced light production when the reaction mixture contained >∼8 μM ATP. The time course of light production was changed from a flash pattern to a constant light output during incubations of 8 μM) were u...

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Veröffentlicht in:Archives of biochemistry and biophysics 1994-11, Vol.314 (2), p.261-267
Hauptverfasser: Ford, S.R., Chenault, K.D., Hall, M.S., Pangburn, S.J., Leach, F.R.
Format: Artikel
Sprache:eng
Schlagworte:
Adenine Nucleotides - pharmacology
Adenosine - analogs & derivatives
Adenosine - pharmacology
Adenosine Triphosphate - analogs & derivatives
Adenosine Triphosphate - metabolism
Adenosine Triphosphate - pharmacology
Affinity Labels - pharmacology
Animals
Coleoptera - enzymology
Enzyme Activation
Kinetics
Light
Luciferases - metabolism
Luminescent Measurements
Time Factors
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Zusammenfassung:Addition of periodate-oxidized ATP (oATP) to firefly luciferase-containing reaction mixtures enhanced light production when the reaction mixture contained >∼8 μM ATP. The time course of light production was changed from a flash pattern to a constant light output during incubations of 8 μM) were used. There is little effect of oATP on firefly luciferase activity at low ATP concentrations (
ISSN:0003-9861
1096-0384
DOI:10.1006/abbi.1994.1440