Defective gamma-interferon production in peripheral blood leukocytes of patients with acute tuberculosis
Production of interferon (IFN)-gamma by peripheral blood leukocytes (PBL) was examined in cultures of unseparated fresh whole blood exposed to phytohemagglutinin (PHA), concanavalin A (Con A), or pokeweed mitogen (PWM). The yield of IFN-gamma was measured by a newly developed immunoradiometric assay...
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| Veröffentlicht in: | Journal of clinical immunology 1986-03, Vol.6 (2), p.146-151 |
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| container_title | Journal of clinical immunology |
| container_volume | 6 |
| creator | VILCEK, J KLION, A HENRIKSEN-DESTEFANO, H ZEMTSOV, A DAVIDSON, D. M DAVIDSON, M FRIEDMANKIEN, A. E JUNMING LE |
| description | Production of interferon (IFN)-gamma by peripheral blood leukocytes (PBL) was examined in cultures of unseparated fresh whole blood exposed to phytohemagglutinin (PHA), concanavalin A (Con A), or pokeweed mitogen (PWM). The yield of IFN-gamma was measured by a newly developed immunoradiometric assay. Nine of 14 patients with acute pulmonary tuberculosis (TB) showed a depressed IFN-gamma response to Con A and/or PWM. Only four of these TB patients also showed a depressed IFN-gamma response to PHA. Stimulation of the patients' PBL cultures with PHA in the presence of exogenous interleukin 2 (IL 2) produced normal IFN-gamma yields in all but the most severely depressed patients. PBL cultures of TB patients with defective IFN-gamma production in response to mitogenic lectins also produced less IFN-gamma after stimulation with tuberculin PPD. Although some patients showed a moderate degree of lymphopenia, their OKT4/T8 lymphocyte ratios were mostly normal or close to normal, with the notable exception of one TB patient who has been diagnosed to have the acquired immune deficiency syndrome (AIDS). |
| doi_str_mv | 10.1007/BF00918747 |
| format | Article |
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PBL cultures of TB patients with defective IFN-gamma production in response to mitogenic lectins also produced less IFN-gamma after stimulation with tuberculin PPD. 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M</creatorcontrib><creatorcontrib>DAVIDSON, M</creatorcontrib><creatorcontrib>FRIEDMANKIEN, A. E</creatorcontrib><creatorcontrib>JUNMING LE</creatorcontrib><title>Defective gamma-interferon production in peripheral blood leukocytes of patients with acute tuberculosis</title><title>Journal of clinical immunology</title><addtitle>J Clin Immunol</addtitle><description>Production of interferon (IFN)-gamma by peripheral blood leukocytes (PBL) was examined in cultures of unseparated fresh whole blood exposed to phytohemagglutinin (PHA), concanavalin A (Con A), or pokeweed mitogen (PWM). The yield of IFN-gamma was measured by a newly developed immunoradiometric assay. Nine of 14 patients with acute pulmonary tuberculosis (TB) showed a depressed IFN-gamma response to Con A and/or PWM. Only four of these TB patients also showed a depressed IFN-gamma response to PHA. Stimulation of the patients' PBL cultures with PHA in the presence of exogenous interleukin 2 (IL 2) produced normal IFN-gamma yields in all but the most severely depressed patients. PBL cultures of TB patients with defective IFN-gamma production in response to mitogenic lectins also produced less IFN-gamma after stimulation with tuberculin PPD. Although some patients showed a moderate degree of lymphopenia, their OKT4/T8 lymphocyte ratios were mostly normal or close to normal, with the notable exception of one TB patient who has been diagnosed to have the acquired immune deficiency syndrome (AIDS).</description><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Concanavalin A - pharmacology</subject><subject>Female</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Infectious diseases</subject><subject>Interferon-gamma - blood</subject><subject>Interleukin-2 - immunology</subject><subject>Leukocytes - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Pokeweed Mitogens - pharmacology</subject><subject>T-Lymphocytes - immunology</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Tuberculosis, Pulmonary - immunology</subject><issn>0271-9142</issn><issn>1573-2592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1r3DAQhkVp2G4-Lr0XdCg9BJyMvizrmM-2EMglORtZHnWV2NZGklP238clS3qaGZ6Hl-El5CuDMwagzy9vAQxrtNSfyJopLSquDP9M1sA1qwyT_As5zPkJAETN1YqsBDS1qNWabK7RoyvhFekfO462ClPB5DHFiW5T7OeFLWtYLkxhu8FkB9oNMfZ0wPk5ul3BTKOnW1sCTiXTv6FsqHVzQVrmDpObh5hDPiYH3g4ZT_bziDze3jxc_aru7n_-vrq4q5xgrFQdd4DWoGicVc7rBoEz3xnDuRJ9LRFk45nnXnPnRO-UYGCZlAaUlxIacUR-vOcu37_MmEs7huxwGOyEcc6trhsluDGLePouuhRzTujbbQqjTbuWQfuv1vZ_rYv8bZ86dyP2H-q-x4V_33ObnR18spML-UNrtAApuXgDq6OAeQ</recordid><startdate>19860301</startdate><enddate>19860301</enddate><creator>VILCEK, J</creator><creator>KLION, A</creator><creator>HENRIKSEN-DESTEFANO, H</creator><creator>ZEMTSOV, A</creator><creator>DAVIDSON, D. M</creator><creator>DAVIDSON, M</creator><creator>FRIEDMANKIEN, A. E</creator><creator>JUNMING LE</creator><general>Kluwer/Plenum</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860301</creationdate><title>Defective gamma-interferon production in peripheral blood leukocytes of patients with acute tuberculosis</title><author>VILCEK, J ; KLION, A ; HENRIKSEN-DESTEFANO, H ; ZEMTSOV, A ; DAVIDSON, D. M ; DAVIDSON, M ; FRIEDMANKIEN, A. E ; JUNMING LE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-b2c0ea9e38ca5cf78e021fb992253d64e048f1f2f72cc3dc5310a144905f44083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adult</topic><topic>AIDS/HIV</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Concanavalin A - pharmacology</topic><topic>Female</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Infectious diseases</topic><topic>Interferon-gamma - blood</topic><topic>Interleukin-2 - immunology</topic><topic>Leukocytes - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Pokeweed Mitogens - pharmacology</topic><topic>T-Lymphocytes - immunology</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Tuberculosis, Pulmonary - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VILCEK, J</creatorcontrib><creatorcontrib>KLION, A</creatorcontrib><creatorcontrib>HENRIKSEN-DESTEFANO, H</creatorcontrib><creatorcontrib>ZEMTSOV, A</creatorcontrib><creatorcontrib>DAVIDSON, D. 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E</au><au>JUNMING LE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Defective gamma-interferon production in peripheral blood leukocytes of patients with acute tuberculosis</atitle><jtitle>Journal of clinical immunology</jtitle><addtitle>J Clin Immunol</addtitle><date>1986-03-01</date><risdate>1986</risdate><volume>6</volume><issue>2</issue><spage>146</spage><epage>151</epage><pages>146-151</pages><issn>0271-9142</issn><eissn>1573-2592</eissn><coden>JCIMDO</coden><abstract>Production of interferon (IFN)-gamma by peripheral blood leukocytes (PBL) was examined in cultures of unseparated fresh whole blood exposed to phytohemagglutinin (PHA), concanavalin A (Con A), or pokeweed mitogen (PWM). The yield of IFN-gamma was measured by a newly developed immunoradiometric assay. Nine of 14 patients with acute pulmonary tuberculosis (TB) showed a depressed IFN-gamma response to Con A and/or PWM. Only four of these TB patients also showed a depressed IFN-gamma response to PHA. Stimulation of the patients' PBL cultures with PHA in the presence of exogenous interleukin 2 (IL 2) produced normal IFN-gamma yields in all but the most severely depressed patients. PBL cultures of TB patients with defective IFN-gamma production in response to mitogenic lectins also produced less IFN-gamma after stimulation with tuberculin PPD. Although some patients showed a moderate degree of lymphopenia, their OKT4/T8 lymphocyte ratios were mostly normal or close to normal, with the notable exception of one TB patient who has been diagnosed to have the acquired immune deficiency syndrome (AIDS).</abstract><cop>New York, NY</cop><pub>Kluwer/Plenum</pub><pmid>3086365</pmid><doi>10.1007/BF00918747</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0271-9142 |
| ispartof | Journal of clinical immunology, 1986-03, Vol.6 (2), p.146-151 |
| issn | 0271-9142 1573-2592 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult AIDS/HIV Bacterial diseases Biological and medical sciences Concanavalin A - pharmacology Female Human bacterial diseases Humans In Vitro Techniques Infectious diseases Interferon-gamma - blood Interleukin-2 - immunology Leukocytes - immunology Male Medical sciences Middle Aged Phytohemagglutinins - pharmacology Pokeweed Mitogens - pharmacology T-Lymphocytes - immunology Tuberculosis and atypical mycobacterial infections Tuberculosis, Pulmonary - immunology |
| title | Defective gamma-interferon production in peripheral blood leukocytes of patients with acute tuberculosis |
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