Incorporation and turnover of labeled exogenous tubulin in the mitotic spindles of Chaetopterus oocytes and HeLa cells

The incorporation of tubulin into mitotic spindles in situ was studied by incubating permeabilized mitotic cells in solutions containing [3H]GTP‐labeled or dichlorotriazinylamino fluorescein (DTAF)‐labeled tubulin. Metaphase HeLa cells or spindle‐containing “minicells” from Chaetopterus oocytes were...

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Veröffentlicht in:	Cell motility and the cytoskeleton 1986, Vol.6 (2), p.114-121
Hauptverfasser:	Goode, Dennis, Sarma, Vidya
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Annelida - ultrastructure assembly polarity Cell Membrane Permeability Chaetopterus Detergents Fluoresceins Fluorescent Dyes Guanosine Diphosphate - metabolism Guanosine Triphosphate - metabolism HeLa cells HeLa Cells - ultrastructure Humans Kinetics Microscopy, Electron microtubules Microtubules - metabolism mitosis Oocytes - ultrastructure Polyethylene Glycols Spindle Apparatus - metabolism Tubulin - metabolism tubulin incorporation
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