Characterization of DNA sequences associated with residual nuclei of Saccharomyces cerevisiae
We have used two different approaches to determine whether particular DNA sequences are specifically associated with high-salt-treated residual nuclei of Saccharomyces cerevisiae. First, libraries of yeast DNA in phage λ were probed with nick-translated total nuclear or residual nuclear DNA from uns...
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| Veröffentlicht in: | Experimental cell research 1986-07, Vol.165 (1), p.29-40 |
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| creator | Potashkin, Judith A. Huberman, Joel A. |
| description | We have used two different approaches to determine whether particular DNA sequences are specifically associated with high-salt-treated residual nuclei of
Saccharomyces cerevisiae. First, libraries of yeast DNA in phage λ were probed with nick-translated total nuclear or residual nuclear DNA from unsynchronized yeast cells. None of the plaques gave a significantly stronger or weaker signal with the residual nuclear probe than with the total nuclear probe. Second, DNA was purified from whole nuclei or residual nuclei which had been isolated from cells in G1, G1/S, early S, or nuclear division. This DNA was “dot-blotted” and then probed with specific yeast DNA sequences. Ribosomal DNA was 2- to 3-fold enriched in residual nuclei in late G1, G1/S, and early S, and 2 μm plasmid DNA sequences were 3- to 5-fold depleted during nuclear division and early G1. However,
ARS1, TRP1, CEN6, and a telomere sequence were neither enriched nor depleted at any time during the cell cycle. |
| doi_str_mv | 10.1016/0014-4827(86)90530-6 |
| format | Article |
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Saccharomyces cerevisiae. First, libraries of yeast DNA in phage λ were probed with nick-translated total nuclear or residual nuclear DNA from unsynchronized yeast cells. None of the plaques gave a significantly stronger or weaker signal with the residual nuclear probe than with the total nuclear probe. Second, DNA was purified from whole nuclei or residual nuclei which had been isolated from cells in G1, G1/S, early S, or nuclear division. This DNA was “dot-blotted” and then probed with specific yeast DNA sequences. Ribosomal DNA was 2- to 3-fold enriched in residual nuclei in late G1, G1/S, and early S, and 2 μm plasmid DNA sequences were 3- to 5-fold depleted during nuclear division and early G1. However,
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Saccharomyces cerevisiae. First, libraries of yeast DNA in phage λ were probed with nick-translated total nuclear or residual nuclear DNA from unsynchronized yeast cells. None of the plaques gave a significantly stronger or weaker signal with the residual nuclear probe than with the total nuclear probe. Second, DNA was purified from whole nuclei or residual nuclei which had been isolated from cells in G1, G1/S, early S, or nuclear division. This DNA was “dot-blotted” and then probed with specific yeast DNA sequences. Ribosomal DNA was 2- to 3-fold enriched in residual nuclei in late G1, G1/S, and early S, and 2 μm plasmid DNA sequences were 3- to 5-fold depleted during nuclear division and early G1. However,
ARS1, TRP1, CEN6, and a telomere sequence were neither enriched nor depleted at any time during the cell cycle.</description><subject>Action of physical and chemical agents</subject><subject>ADN</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle</subject><subject>Cell Nucleus - physiology</subject><subject>DNA</subject><subject>DNA, Fungal - genetics</subject><subject>Drosophila - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>NOYAU CELLULAIRE</subject><subject>Nucleic Acid Hybridization</subject><subject>NUCLEO</subject><subject>NUCLEUS</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - genetics</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhS0EKtPCH0AgZYEQLALXb2eDVE15SRUsSpfIcm5uqFFm0tpJUfn1OMxolsDKi_Odo3N9GHvK4TUHbt4AcFUrJ-xLZ141oCXU5h5bcWigFkqI-2x1QB6y45x_AIBz3ByxI6l5I7RbsW_rq5ACTpTirzDFcVuNfXX2-bTKdDPTFilXIecRY5ioq37G6apKlGM3h6HazjhQXAwXAbHkjJu7xYCU6DbmGOgRe9CHIdPj_XvCLt-_-7r-WJ9_-fBpfXpeozJ2qqW1ukXTNqi1pr605NhJ20GLopxDvQmGo2qbruUgW6cDl8Iq07agABsrT9iLXe51GkvtPPlNzEjDELY0ztlb45SSXPwT5MoI0zj4D1CDslwWUO1ATGPOiXp_neImpDvPwS87-WUEv4zgnfF_dvKm2J7t8-d2Q93BtB-m6M_3esgYhj6FLcZ8wGzjhNO6YE92WB9GH76nglxeOAtcy-XYtzuRytffRko-Y1w27WIinHw3xr-X_A0EnrY8</recordid><startdate>19860701</startdate><enddate>19860701</enddate><creator>Potashkin, Judith A.</creator><creator>Huberman, Joel A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19860701</creationdate><title>Characterization of DNA sequences associated with residual nuclei of Saccharomyces cerevisiae</title><author>Potashkin, Judith A. ; Huberman, Joel A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-3775bc6b9c555ef0081cd37d0bc2109ef6a61c4b9db103b85a132746bb040c973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Action of physical and chemical agents</topic><topic>ADN</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle</topic><topic>Cell Nucleus - physiology</topic><topic>DNA</topic><topic>DNA, Fungal - genetics</topic><topic>Drosophila - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>NOYAU CELLULAIRE</topic><topic>Nucleic Acid Hybridization</topic><topic>NUCLEO</topic><topic>NUCLEUS</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Potashkin, Judith A.</creatorcontrib><creatorcontrib>Huberman, Joel A.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Potashkin, Judith A.</au><au>Huberman, Joel A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of DNA sequences associated with residual nuclei of Saccharomyces cerevisiae</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1986-07-01</date><risdate>1986</risdate><volume>165</volume><issue>1</issue><spage>29</spage><epage>40</epage><pages>29-40</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>We have used two different approaches to determine whether particular DNA sequences are specifically associated with high-salt-treated residual nuclei of
Saccharomyces cerevisiae. First, libraries of yeast DNA in phage λ were probed with nick-translated total nuclear or residual nuclear DNA from unsynchronized yeast cells. None of the plaques gave a significantly stronger or weaker signal with the residual nuclear probe than with the total nuclear probe. Second, DNA was purified from whole nuclei or residual nuclei which had been isolated from cells in G1, G1/S, early S, or nuclear division. This DNA was “dot-blotted” and then probed with specific yeast DNA sequences. Ribosomal DNA was 2- to 3-fold enriched in residual nuclei in late G1, G1/S, and early S, and 2 μm plasmid DNA sequences were 3- to 5-fold depleted during nuclear division and early G1. However,
ARS1, TRP1, CEN6, and a telomere sequence were neither enriched nor depleted at any time during the cell cycle.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>3519258</pmid><doi>10.1016/0014-4827(86)90530-6</doi><tpages>12</tpages></addata></record> |
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| ispartof | Experimental cell research, 1986-07, Vol.165 (1), p.29-40 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Action of physical and chemical agents ADN Base Sequence Biological and medical sciences Cell Cycle Cell Nucleus - physiology DNA DNA, Fungal - genetics Drosophila - genetics Fundamental and applied biological sciences. Psychology Microbiology Mycology NOYAU CELLULAIRE Nucleic Acid Hybridization NUCLEO NUCLEUS Repetitive Sequences, Nucleic Acid SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics |
| title | Characterization of DNA sequences associated with residual nuclei of Saccharomyces cerevisiae |
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