Further studies on the size and composition of the chick embryo fibroblast cytosolic DNA complex

Further studies on the size and composition of the chick embryo fibroblast cytosolic DNA complex

1. 1. The chick embryo fibroblast cytosolic DNA complex shows anomalous elution behaviour on agarose gel column chromatography. 2. 2. The indicated molecular size varies between 5 × 10 5 dalton (higher exclusion limit gels) and 1.4 × 10 6 dalton (lower exclusion limit gels). 3. 3.Chromatography on l...

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Veröffentlicht in:International journal of biochemistry 1986, Vol.18 (5), p.423-429
Hauptverfasser: Challen, Christine, Adams, David H.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cells, Cultured
Chick Embryo
Chromatography, Agarose
Cytosol - metabolism
DNA - metabolism
Fibroblasts - metabolism
Lysine - metabolism
Methionine - metabolism
Uridine - metabolism
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Zusammenfassung:1. 1. The chick embryo fibroblast cytosolic DNA complex shows anomalous elution behaviour on agarose gel column chromatography. 2. 2. The indicated molecular size varies between 5 × 10 5 dalton (higher exclusion limit gels) and 1.4 × 10 6 dalton (lower exclusion limit gels). 3. 3.Chromatography on lower exclusion limit gels shows the [ 3H]thymidine labelled (DNA) complex as a sharp peak, coincident with a peak of [ 3H]uridine and [ 3H]lysine labelling and similar pulse labelling patterns for the three precursors but with DNA labelling lagging behind RNA and protein. 4. 4. Both cultured and uncultured cell cytosols show an A 260 peak coincident with the [ 3H]precursor labelling peaks.
ISSN:0020-711X
DOI:10.1016/0020-711X(86)90184-9