Inhibition of Radiation-Enhanced Expression of Integrin and Metastatic Potential in B16 Melanoma Cells by a Lipoxygenase Inhibitor
Low-dose γ radiation stimulates expression of phenotypic characteristics in B16 melanoma cells which regulate metastatic potential. A transient increase in the expression of an integrin receptor (α IIbβ 3) was observed after exposure of B16 melanoma cells to 0.25 to 2.0 Gy of γ radiation. This incre...
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| Veröffentlicht in: | Radiation Research 1994-12, Vol.140 (3), p.410-418 |
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| creator | Onoda, J. M. Kantak, S. S. Piechocki, M. P. Awad, W. Chea, R. Liu, B. Honn, K. V. |
| description | Low-dose γ radiation stimulates expression of phenotypic characteristics in B16 melanoma cells which regulate metastatic potential. A transient increase in the expression of an integrin receptor (α IIbβ 3) was observed after exposure of B16 melanoma cells to 0.25 to 2.0 Gy of γ radiation. This increased receptor expression resulted in enhanced adhesion of tumor cells to fibronectin in vitro and increased experimentally induced metastasis in vivo. In this report, we determined a role for the 12-lipoxygenase metabolite, 12-HETE, in radiation-enhanced metastasis. A significant increase in biosynthesis of 12-HETE in B16 melanoma cells was detected |
| doi_str_mv | 10.2307/3579120 |
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M. ; Kantak, S. S. ; Piechocki, M. P. ; Awad, W. ; Chea, R. ; Liu, B. ; Honn, K. V.</creator><creatorcontrib>Onoda, J. M. ; Kantak, S. S. ; Piechocki, M. P. ; Awad, W. ; Chea, R. ; Liu, B. ; Honn, K. V.</creatorcontrib><description>Low-dose γ radiation stimulates expression of phenotypic characteristics in B16 melanoma cells which regulate metastatic potential. A transient increase in the expression of an integrin receptor (α IIbβ 3) was observed after exposure of B16 melanoma cells to 0.25 to 2.0 Gy of γ radiation. This increased receptor expression resulted in enhanced adhesion of tumor cells to fibronectin in vitro and increased experimentally induced metastasis in vivo. In this report, we determined a role for the 12-lipoxygenase metabolite, 12-HETE, in radiation-enhanced metastasis. A significant increase in biosynthesis of 12-HETE in B16 melanoma cells was detected <5 min after exposure to 0.5 Gy γ radiation. We then determined that radiation-enhanced expression of α IIbβ 3 integrin and adhesion of B16 melanoma cells to fibronectin in vitro and metastasis in vivo were reduced by treatment of the cells with the lipoxygenase inhibitor NDGA prior to irradiation. These findings suggest that low-dose radiation, at levels comparable to those used in fractionated or hyperfractionated radiotherapy, increases the metastatic potential of surviving tumor cells via a rapid and transient alteration in lipoxygenase metabolism of arachidonic acid and surface expression of an integrin receptor.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3579120</identifier><identifier>PMID: 7972695</identifier><identifier>CODEN: RAREAE</identifier><language>eng</language><publisher>Oak Brook, Il: Radiation Research Society</publisher><subject>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid ; ADHESION ; Animals ; ARACHIDONIC ACID ; Biological and medical sciences ; Biological effects of radiation ; BIOLOGY AND MEDICINE, APPLIED STUDIES ; BIOLOGY AND MEDICINE, BASIC STUDIES ; BIOSYNTHESIS ; Cell adhesion ; Cell Adhesion - radiation effects ; Cellular metabolism ; DOSE-RESPONSE RELATIONSHIPS ; Endothelial cells ; Fibronectins - metabolism ; Fibrosis ; Fundamental and applied biological sciences. Psychology ; GAMMA RADIATION ; Gamma Rays ; Hydroxyeicosatetraenoic Acids - metabolism ; INHIBITION ; Integrins ; Integrins - metabolism ; Ionizing radiations ; Irradiation ; Lipoxygenase Inhibitors - pharmacology ; Male ; Masoprocol - pharmacology ; Melanoma, Experimental - metabolism ; Melanoma, Experimental - pathology ; MELANOMAS ; METABOLISM ; METASTABLE STATES ; Metastasis ; Mice ; Mice, Inbred C57BL ; Neoplasm Metastasis ; OXYGENASES ; RADIOTHERAPY ; RECEPTORS ; Signal Transduction - radiation effects ; Space life sciences ; STIMULATION ; Tissues, organs and organisms biophysics ; TUMOR CELLS ; Tumor Cells, Cultured - radiation effects ; Tumors</subject><ispartof>Radiation Research, 1994-12, Vol.140 (3), p.410-418</ispartof><rights>Copyright 1994 The Radiation Research Society</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-8a88ccceb9007c71b3c60177fd60b5151463fdfa630ec78e7f747f271d817cdd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3579120$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3579120$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,885,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3348966$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7972695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/50633$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Onoda, J. M.</creatorcontrib><creatorcontrib>Kantak, S. S.</creatorcontrib><creatorcontrib>Piechocki, M. P.</creatorcontrib><creatorcontrib>Awad, W.</creatorcontrib><creatorcontrib>Chea, R.</creatorcontrib><creatorcontrib>Liu, B.</creatorcontrib><creatorcontrib>Honn, K. V.</creatorcontrib><title>Inhibition of Radiation-Enhanced Expression of Integrin and Metastatic Potential in B16 Melanoma Cells by a Lipoxygenase Inhibitor</title><title>Radiation Research</title><addtitle>Radiat Res</addtitle><description>Low-dose γ radiation stimulates expression of phenotypic characteristics in B16 melanoma cells which regulate metastatic potential. A transient increase in the expression of an integrin receptor (α IIbβ 3) was observed after exposure of B16 melanoma cells to 0.25 to 2.0 Gy of γ radiation. This increased receptor expression resulted in enhanced adhesion of tumor cells to fibronectin in vitro and increased experimentally induced metastasis in vivo. In this report, we determined a role for the 12-lipoxygenase metabolite, 12-HETE, in radiation-enhanced metastasis. A significant increase in biosynthesis of 12-HETE in B16 melanoma cells was detected <5 min after exposure to 0.5 Gy γ radiation. We then determined that radiation-enhanced expression of α IIbβ 3 integrin and adhesion of B16 melanoma cells to fibronectin in vitro and metastasis in vivo were reduced by treatment of the cells with the lipoxygenase inhibitor NDGA prior to irradiation. These findings suggest that low-dose radiation, at levels comparable to those used in fractionated or hyperfractionated radiotherapy, increases the metastatic potential of surviving tumor cells via a rapid and transient alteration in lipoxygenase metabolism of arachidonic acid and surface expression of an integrin receptor.</description><subject>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid</subject><subject>ADHESION</subject><subject>Animals</subject><subject>ARACHIDONIC ACID</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>BIOLOGY AND MEDICINE, APPLIED STUDIES</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>BIOSYNTHESIS</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - radiation effects</subject><subject>Cellular metabolism</subject><subject>DOSE-RESPONSE RELATIONSHIPS</subject><subject>Endothelial cells</subject><subject>Fibronectins - metabolism</subject><subject>Fibrosis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAMMA RADIATION</subject><subject>Gamma Rays</subject><subject>Hydroxyeicosatetraenoic Acids - metabolism</subject><subject>INHIBITION</subject><subject>Integrins</subject><subject>Integrins - metabolism</subject><subject>Ionizing radiations</subject><subject>Irradiation</subject><subject>Lipoxygenase Inhibitors - pharmacology</subject><subject>Male</subject><subject>Masoprocol - pharmacology</subject><subject>Melanoma, Experimental - metabolism</subject><subject>Melanoma, Experimental - pathology</subject><subject>MELANOMAS</subject><subject>METABOLISM</subject><subject>METASTABLE STATES</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neoplasm Metastasis</subject><subject>OXYGENASES</subject><subject>RADIOTHERAPY</subject><subject>RECEPTORS</subject><subject>Signal Transduction - radiation effects</subject><subject>Space life sciences</subject><subject>STIMULATION</subject><subject>Tissues, organs and organisms biophysics</subject><subject>TUMOR CELLS</subject><subject>Tumor Cells, Cultured - radiation effects</subject><subject>Tumors</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10cGKFDEQBuAgyjqu4hMIOYieWpNOd5I-6jDqwIgiem7SSWUnS08ypjKwc_XJzTLNevIUiv-jilQR8pKzd61g6r3o1cBb9ois-CB003ese0xWjAnRqF6rp-QZ4i2rNZfDFblSg2rl0K_In23chymUkCJNnv4wLpj7otnEvYkWHN3cHTMgLmAbC9zkEKmJjn6FYrBUb-n3VCCWYGZas49c1mw2MR0MXcM8I53O1NBdOKa78w1Eg0CXwSk_J0-8mRFeLO81-fVp83P9pdl9-7xdf9g1tmv70mijtbUWpoExZRWfhJWMK-WdZFPPe95J4Z03UjCwSoPyqlO-Vdxprqxz4prQS9-EJYxoQwG7tylGsGXsmRSikjcXcszp9wmwjIeAtn7AREgnHJXUndCSVfj2Am1OiBn8eMzhYPJ55Gy8P8i4HKTKV0vL03QA9-CWC9T89ZIbtGb2uW494AMTotODlP_YLdaV_XfaX52ZnYE</recordid><startdate>19941201</startdate><enddate>19941201</enddate><creator>Onoda, J. M.</creator><creator>Kantak, S. S.</creator><creator>Piechocki, M. P.</creator><creator>Awad, W.</creator><creator>Chea, R.</creator><creator>Liu, B.</creator><creator>Honn, K. V.</creator><general>Radiation Research Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19941201</creationdate><title>Inhibition of Radiation-Enhanced Expression of Integrin and Metastatic Potential in B16 Melanoma Cells by a Lipoxygenase Inhibitor</title><author>Onoda, J. M. ; Kantak, S. S. ; Piechocki, M. P. ; Awad, W. ; Chea, R. ; Liu, B. ; Honn, K. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-8a88ccceb9007c71b3c60177fd60b5151463fdfa630ec78e7f747f271d817cdd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid</topic><topic>ADHESION</topic><topic>Animals</topic><topic>ARACHIDONIC ACID</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>BIOLOGY AND MEDICINE, APPLIED STUDIES</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>BIOSYNTHESIS</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - radiation effects</topic><topic>Cellular metabolism</topic><topic>DOSE-RESPONSE RELATIONSHIPS</topic><topic>Endothelial cells</topic><topic>Fibronectins - metabolism</topic><topic>Fibrosis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAMMA RADIATION</topic><topic>Gamma Rays</topic><topic>Hydroxyeicosatetraenoic Acids - metabolism</topic><topic>INHIBITION</topic><topic>Integrins</topic><topic>Integrins - metabolism</topic><topic>Ionizing radiations</topic><topic>Irradiation</topic><topic>Lipoxygenase Inhibitors - pharmacology</topic><topic>Male</topic><topic>Masoprocol - pharmacology</topic><topic>Melanoma, Experimental - metabolism</topic><topic>Melanoma, Experimental - pathology</topic><topic>MELANOMAS</topic><topic>METABOLISM</topic><topic>METASTABLE STATES</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Neoplasm Metastasis</topic><topic>OXYGENASES</topic><topic>RADIOTHERAPY</topic><topic>RECEPTORS</topic><topic>Signal Transduction - radiation effects</topic><topic>Space life sciences</topic><topic>STIMULATION</topic><topic>Tissues, organs and organisms biophysics</topic><topic>TUMOR CELLS</topic><topic>Tumor Cells, Cultured - radiation effects</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Onoda, J. M.</creatorcontrib><creatorcontrib>Kantak, S. S.</creatorcontrib><creatorcontrib>Piechocki, M. P.</creatorcontrib><creatorcontrib>Awad, W.</creatorcontrib><creatorcontrib>Chea, R.</creatorcontrib><creatorcontrib>Liu, B.</creatorcontrib><creatorcontrib>Honn, K. V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Radiation Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Onoda, J. M.</au><au>Kantak, S. S.</au><au>Piechocki, M. P.</au><au>Awad, W.</au><au>Chea, R.</au><au>Liu, B.</au><au>Honn, K. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of Radiation-Enhanced Expression of Integrin and Metastatic Potential in B16 Melanoma Cells by a Lipoxygenase Inhibitor</atitle><jtitle>Radiation Research</jtitle><addtitle>Radiat Res</addtitle><date>1994-12-01</date><risdate>1994</risdate><volume>140</volume><issue>3</issue><spage>410</spage><epage>418</epage><pages>410-418</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><coden>RAREAE</coden><abstract>Low-dose γ radiation stimulates expression of phenotypic characteristics in B16 melanoma cells which regulate metastatic potential. A transient increase in the expression of an integrin receptor (α IIbβ 3) was observed after exposure of B16 melanoma cells to 0.25 to 2.0 Gy of γ radiation. This increased receptor expression resulted in enhanced adhesion of tumor cells to fibronectin in vitro and increased experimentally induced metastasis in vivo. In this report, we determined a role for the 12-lipoxygenase metabolite, 12-HETE, in radiation-enhanced metastasis. A significant increase in biosynthesis of 12-HETE in B16 melanoma cells was detected <5 min after exposure to 0.5 Gy γ radiation. We then determined that radiation-enhanced expression of α IIbβ 3 integrin and adhesion of B16 melanoma cells to fibronectin in vitro and metastasis in vivo were reduced by treatment of the cells with the lipoxygenase inhibitor NDGA prior to irradiation. These findings suggest that low-dose radiation, at levels comparable to those used in fractionated or hyperfractionated radiotherapy, increases the metastatic potential of surviving tumor cells via a rapid and transient alteration in lipoxygenase metabolism of arachidonic acid and surface expression of an integrin receptor.</abstract><cop>Oak Brook, Il</cop><pub>Radiation Research Society</pub><pmid>7972695</pmid><doi>10.2307/3579120</doi><tpages>9</tpages></addata></record> |
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| ispartof | Radiation Research, 1994-12, Vol.140 (3), p.410-418 |
| issn | 0033-7587 1938-5404 |
| language | eng |
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| subjects | 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid ADHESION Animals ARACHIDONIC ACID Biological and medical sciences Biological effects of radiation BIOLOGY AND MEDICINE, APPLIED STUDIES BIOLOGY AND MEDICINE, BASIC STUDIES BIOSYNTHESIS Cell adhesion Cell Adhesion - radiation effects Cellular metabolism DOSE-RESPONSE RELATIONSHIPS Endothelial cells Fibronectins - metabolism Fibrosis Fundamental and applied biological sciences. Psychology GAMMA RADIATION Gamma Rays Hydroxyeicosatetraenoic Acids - metabolism INHIBITION Integrins Integrins - metabolism Ionizing radiations Irradiation Lipoxygenase Inhibitors - pharmacology Male Masoprocol - pharmacology Melanoma, Experimental - metabolism Melanoma, Experimental - pathology MELANOMAS METABOLISM METASTABLE STATES Metastasis Mice Mice, Inbred C57BL Neoplasm Metastasis OXYGENASES RADIOTHERAPY RECEPTORS Signal Transduction - radiation effects Space life sciences STIMULATION Tissues, organs and organisms biophysics TUMOR CELLS Tumor Cells, Cultured - radiation effects Tumors |
| title | Inhibition of Radiation-Enhanced Expression of Integrin and Metastatic Potential in B16 Melanoma Cells by a Lipoxygenase Inhibitor |
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