Entamoeba histolytica: Specific antigen recognized by a monoclonal antibody
Specific antigenic determinants on the membrane surface of Entamoeba histolytica that distinguish it from other Entamoeba species were demonstrated. Evidence for these antigenic determinants was obtained with a monoclonal antibody to E. histolytica which showed not only specificity but also sensitiv...
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| Veröffentlicht in: | Experimental parasitology 1986-06, Vol.61 (3), p.390-397 |
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| container_title | Experimental parasitology |
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| creator | Ortiz-Ortiz, Librado Ximénez, Cecilia Mendoza, Felipe Michalak, Colette Melendro, Emma I. Oliva, Angel |
| description | Specific antigenic determinants on the membrane surface of
Entamoeba histolytica that distinguish it from other
Entamoeba species were demonstrated. Evidence for these antigenic determinants was obtained with a monoclonal antibody to
E. histolytica which showed not only specificity but also sensitivity as demonstrated in enzyme linked immunosorbent assay. Immunofluorescence microscopy showed that the monoclonal antibody recognized an epitope present on the membrane surface of
E. histolytica trophozoites. The epitope detected by the monoclonal antibody was present in three components of different molecular weight. These components may have a common precursor or may be the result of enzymatic degradation under the conditions tested. |
| doi_str_mv | 10.1016/0014-4894(86)90195-5 |
| format | Article |
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Entamoeba histolytica that distinguish it from other
Entamoeba species were demonstrated. Evidence for these antigenic determinants was obtained with a monoclonal antibody to
E. histolytica which showed not only specificity but also sensitivity as demonstrated in enzyme linked immunosorbent assay. Immunofluorescence microscopy showed that the monoclonal antibody recognized an epitope present on the membrane surface of
E. histolytica trophozoites. The epitope detected by the monoclonal antibody was present in three components of different molecular weight. These components may have a common precursor or may be the result of enzymatic degradation under the conditions tested.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/0014-4894(86)90195-5</identifier><identifier>PMID: 2423362</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amibiasis ; Animals ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Antigens, Protozoan - immunology ; Antigens, Surface - immunology ; Biological and medical sciences ; Entamoeba histolytica ; Entamoeba histolytica - immunology ; Entamoeba invadens ; Entamoeba moshkouski ; Enzyme-Linked Immunosorbent Assay ; Epitopes - immunology ; Fluorescent Antibody Technique ; Human protozoal diseases ; Infectious diseases ; Medical sciences ; Molecular Weight ; Monoclonal antibody ; Parasitic diseases ; Protozoa, parasitic ; Protozoal diseases ; Species Specificity ; Tropical medicine</subject><ispartof>Experimental parasitology, 1986-06, Vol.61 (3), p.390-397</ispartof><rights>1986</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-fed3384b40366e6adf0dd508dbe83ea1aef7c5653a0141712f9cb42a35517c353</citedby><cites>FETCH-LOGICAL-c386t-fed3384b40366e6adf0dd508dbe83ea1aef7c5653a0141712f9cb42a35517c353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4894(86)90195-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8780521$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2423362$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ortiz-Ortiz, Librado</creatorcontrib><creatorcontrib>Ximénez, Cecilia</creatorcontrib><creatorcontrib>Mendoza, Felipe</creatorcontrib><creatorcontrib>Michalak, Colette</creatorcontrib><creatorcontrib>Melendro, Emma I.</creatorcontrib><creatorcontrib>Oliva, Angel</creatorcontrib><title>Entamoeba histolytica: Specific antigen recognized by a monoclonal antibody</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>Specific antigenic determinants on the membrane surface of
Entamoeba histolytica that distinguish it from other
Entamoeba species were demonstrated. Evidence for these antigenic determinants was obtained with a monoclonal antibody to
E. histolytica which showed not only specificity but also sensitivity as demonstrated in enzyme linked immunosorbent assay. Immunofluorescence microscopy showed that the monoclonal antibody recognized an epitope present on the membrane surface of
E. histolytica trophozoites. The epitope detected by the monoclonal antibody was present in three components of different molecular weight. These components may have a common precursor or may be the result of enzymatic degradation under the conditions tested.</description><subject>Amibiasis</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Antigens, Protozoan - immunology</subject><subject>Antigens, Surface - immunology</subject><subject>Biological and medical sciences</subject><subject>Entamoeba histolytica</subject><subject>Entamoeba histolytica - immunology</subject><subject>Entamoeba invadens</subject><subject>Entamoeba moshkouski</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - immunology</subject><subject>Fluorescent Antibody Technique</subject><subject>Human protozoal diseases</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Molecular Weight</subject><subject>Monoclonal antibody</subject><subject>Parasitic diseases</subject><subject>Protozoa, parasitic</subject><subject>Protozoal diseases</subject><subject>Species Specificity</subject><subject>Tropical medicine</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P3DAQhi1URLfAP6BSDhUqh4C_4_RQCSEKCCQOwNma2BMwSuwlziItv75ZdrVHTnN4n3k18xByxOgpo0yfUcpkKU0tfxt9UlNWq1LtkBmjNS25lPU3Mtsi38mPnF8ppYZxuUf2uORCaD4jt5dxhD5hA8VLyGPqlmNw8Kd4mKMLbXAFxDE8YywGdOk5hg_0RbMsoOhTTK5LEbpPpEl-eUB2W-gyHm7mPnn6d_l4cV3e3V_dXJzflU4YPZYteiGMbCQVWqMG31LvFTW-QSMQGGBbOaWVgOl6VjHe1q6RHIRSrHJCiX1yvO6dD-ltgXm0fcgOuw4ipkW2lTbTe7yeQLkG3ZByHrC18yH0MCwto3bl0K4E2ZUga7T9dGhX_T83_YumR79d2kib8l-bHLKDrh0gupC3mKkMVZxN2N81hpOL94CDzS5gdOjDJHO0PoWv7_gPcbmNLw</recordid><startdate>19860601</startdate><enddate>19860601</enddate><creator>Ortiz-Ortiz, Librado</creator><creator>Ximénez, Cecilia</creator><creator>Mendoza, Felipe</creator><creator>Michalak, Colette</creator><creator>Melendro, Emma I.</creator><creator>Oliva, Angel</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860601</creationdate><title>Entamoeba histolytica: Specific antigen recognized by a monoclonal antibody</title><author>Ortiz-Ortiz, Librado ; Ximénez, Cecilia ; Mendoza, Felipe ; Michalak, Colette ; Melendro, Emma I. ; Oliva, Angel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-fed3384b40366e6adf0dd508dbe83ea1aef7c5653a0141712f9cb42a35517c353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Amibiasis</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>Antigens, Protozoan - immunology</topic><topic>Antigens, Surface - immunology</topic><topic>Biological and medical sciences</topic><topic>Entamoeba histolytica</topic><topic>Entamoeba histolytica - immunology</topic><topic>Entamoeba invadens</topic><topic>Entamoeba moshkouski</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - immunology</topic><topic>Fluorescent Antibody Technique</topic><topic>Human protozoal diseases</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Molecular Weight</topic><topic>Monoclonal antibody</topic><topic>Parasitic diseases</topic><topic>Protozoa, parasitic</topic><topic>Protozoal diseases</topic><topic>Species Specificity</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ortiz-Ortiz, Librado</creatorcontrib><creatorcontrib>Ximénez, Cecilia</creatorcontrib><creatorcontrib>Mendoza, Felipe</creatorcontrib><creatorcontrib>Michalak, Colette</creatorcontrib><creatorcontrib>Melendro, Emma I.</creatorcontrib><creatorcontrib>Oliva, Angel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ortiz-Ortiz, Librado</au><au>Ximénez, Cecilia</au><au>Mendoza, Felipe</au><au>Michalak, Colette</au><au>Melendro, Emma I.</au><au>Oliva, Angel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Entamoeba histolytica: Specific antigen recognized by a monoclonal antibody</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>1986-06-01</date><risdate>1986</risdate><volume>61</volume><issue>3</issue><spage>390</spage><epage>397</epage><pages>390-397</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>Specific antigenic determinants on the membrane surface of
Entamoeba histolytica that distinguish it from other
Entamoeba species were demonstrated. Evidence for these antigenic determinants was obtained with a monoclonal antibody to
E. histolytica which showed not only specificity but also sensitivity as demonstrated in enzyme linked immunosorbent assay. Immunofluorescence microscopy showed that the monoclonal antibody recognized an epitope present on the membrane surface of
E. histolytica trophozoites. The epitope detected by the monoclonal antibody was present in three components of different molecular weight. These components may have a common precursor or may be the result of enzymatic degradation under the conditions tested.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2423362</pmid><doi>10.1016/0014-4894(86)90195-5</doi><tpages>8</tpages></addata></record> |
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| ispartof | Experimental parasitology, 1986-06, Vol.61 (3), p.390-397 |
| issn | 0014-4894 1090-2449 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Amibiasis Animals Antibodies, Monoclonal - immunology Antibody Specificity Antigens, Protozoan - immunology Antigens, Surface - immunology Biological and medical sciences Entamoeba histolytica Entamoeba histolytica - immunology Entamoeba invadens Entamoeba moshkouski Enzyme-Linked Immunosorbent Assay Epitopes - immunology Fluorescent Antibody Technique Human protozoal diseases Infectious diseases Medical sciences Molecular Weight Monoclonal antibody Parasitic diseases Protozoa, parasitic Protozoal diseases Species Specificity Tropical medicine |
| title | Entamoeba histolytica: Specific antigen recognized by a monoclonal antibody |
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