Efficient Neutralization of Primary Isolates of HIV-1 by a Recombinant Human Monoclonal Antibody
The ability of antibodies to neutralize diverse primary isolates of human immunodeficiency virus-type 1 in vitro has been questioned, with implications for the likely efficacy of vaccines. A recombinant human antibody to envelope glycoprotein gp120 was generated and used to show that primary isolate...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1994-11, Vol.266 (5187), p.1024-1027 |
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| creator | Burton, Dennis R. Pyati, Jayashree Koduri, Raju Sharp, Stephen J. Thornton, George B. Paul W. H. I. Parren Lynette S. W. Sawyer Hendry, R. Michael Dunlop, Nancy Nara, Peter L. Lamacchia, Michael Garratty, Eileen Stiehm, E. Richard Bryson, Yvonne J. Cao, Yunzhen Moore, John P. Ho, David D. Barbas, Carlos F. |
| description | The ability of antibodies to neutralize diverse primary isolates of human immunodeficiency virus-type 1 in vitro has been questioned, with implications for the likely efficacy of vaccines. A recombinant human antibody to envelope glycoprotein gp120 was generated and used to show that primary isolates are not refractory to antibody neutralization. The recombinant antibody neutralized more than 75 percent of the primary isolates tested at concentrations that could be achieved by passive immunization, for example, to interrupt maternal-fetal transmission of virus. The broad specificity and efficacy of the antibody implies the conservation of a structural feature on gp120, which could be important in vaccine design. |
| doi_str_mv | 10.1126/science.7973652 |
| format | Article |
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H. I. Parren ; Lynette S. W. Sawyer ; Hendry, R. Michael ; Dunlop, Nancy ; Nara, Peter L. ; Lamacchia, Michael ; Garratty, Eileen ; Stiehm, E. Richard ; Bryson, Yvonne J. ; Cao, Yunzhen ; Moore, John P. ; Ho, David D. ; Barbas, Carlos F.</creator><creatorcontrib>Burton, Dennis R. ; Pyati, Jayashree ; Koduri, Raju ; Sharp, Stephen J. ; Thornton, George B. ; Paul W. H. I. Parren ; Lynette S. W. Sawyer ; Hendry, R. Michael ; Dunlop, Nancy ; Nara, Peter L. ; Lamacchia, Michael ; Garratty, Eileen ; Stiehm, E. Richard ; Bryson, Yvonne J. ; Cao, Yunzhen ; Moore, John P. ; Ho, David D. ; Barbas, Carlos F.</creatorcontrib><description>The ability of antibodies to neutralize diverse primary isolates of human immunodeficiency virus-type 1 in vitro has been questioned, with implications for the likely efficacy of vaccines. A recombinant human antibody to envelope glycoprotein gp120 was generated and used to show that primary isolates are not refractory to antibody neutralization. The recombinant antibody neutralized more than 75 percent of the primary isolates tested at concentrations that could be achieved by passive immunization, for example, to interrupt maternal-fetal transmission of virus. The broad specificity and efficacy of the antibody implies the conservation of a structural feature on gp120, which could be important in vaccine design.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.7973652</identifier><identifier>PMID: 7973652</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Acquired Immunodeficiency Syndrome - virology ; AIDS ; AIDS vaccines ; AIDS Vaccines - immunology ; AIDS/HIV ; Amino Acid Sequence ; Antibodies ; Antibodies, immunoglobulins ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Biological and medical sciences ; Enzyme linked immunosorbent assay ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; HIV 1 ; HIV Antibodies - immunology ; HIV Core Protein p24 - analysis ; HIV Envelope Protein gp120 - immunology ; HIV-1 - immunology ; HIV-1 - isolation & purification ; human immunodeficiency virus 1 ; Humans ; Immunization, Passive ; Immunoglobulin Fab Fragments - immunology ; Immunoglobulin G - immunology ; Infant ; Infant, Newborn ; Infants ; Libraries ; Male ; Molecular immunology ; Molecular Sequence Data ; Monoclonal antibodies ; Neutralization Tests ; Reactivity ; Recombinant Proteins - immunology ; Vaccination ; Viruses</subject><ispartof>Science (American Association for the Advancement of Science), 1994-11, Vol.266 (5187), p.1024-1027</ispartof><rights>Copyright 1994 American Association for the Advancement of Science</rights><rights>1995 INIST-CNRS</rights><rights>COPYRIGHT 1994 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1994 American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c714t-43afe783fb3308998248e16df370314323da239b99ca1dfe8e711eabcab84db43</citedby><cites>FETCH-LOGICAL-c714t-43afe783fb3308998248e16df370314323da239b99ca1dfe8e711eabcab84db43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2885490$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2885490$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3399025$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7973652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burton, Dennis R.</creatorcontrib><creatorcontrib>Pyati, Jayashree</creatorcontrib><creatorcontrib>Koduri, Raju</creatorcontrib><creatorcontrib>Sharp, Stephen J.</creatorcontrib><creatorcontrib>Thornton, George B.</creatorcontrib><creatorcontrib>Paul W. H. I. Parren</creatorcontrib><creatorcontrib>Lynette S. W. Sawyer</creatorcontrib><creatorcontrib>Hendry, R. Michael</creatorcontrib><creatorcontrib>Dunlop, Nancy</creatorcontrib><creatorcontrib>Nara, Peter L.</creatorcontrib><creatorcontrib>Lamacchia, Michael</creatorcontrib><creatorcontrib>Garratty, Eileen</creatorcontrib><creatorcontrib>Stiehm, E. Richard</creatorcontrib><creatorcontrib>Bryson, Yvonne J.</creatorcontrib><creatorcontrib>Cao, Yunzhen</creatorcontrib><creatorcontrib>Moore, John P.</creatorcontrib><creatorcontrib>Ho, David D.</creatorcontrib><creatorcontrib>Barbas, Carlos F.</creatorcontrib><title>Efficient Neutralization of Primary Isolates of HIV-1 by a Recombinant Human Monoclonal Antibody</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The ability of antibodies to neutralize diverse primary isolates of human immunodeficiency virus-type 1 in vitro has been questioned, with implications for the likely efficacy of vaccines. A recombinant human antibody to envelope glycoprotein gp120 was generated and used to show that primary isolates are not refractory to antibody neutralization. The recombinant antibody neutralized more than 75 percent of the primary isolates tested at concentrations that could be achieved by passive immunization, for example, to interrupt maternal-fetal transmission of virus. The broad specificity and efficacy of the antibody implies the conservation of a structural feature on gp120, which could be important in vaccine design.</description><subject>Acquired Immunodeficiency Syndrome - virology</subject><subject>AIDS</subject><subject>AIDS vaccines</subject><subject>AIDS Vaccines - immunology</subject><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>Antibodies</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>HIV 1</subject><subject>HIV Antibodies - immunology</subject><subject>HIV Core Protein p24 - analysis</subject><subject>HIV Envelope Protein gp120 - immunology</subject><subject>HIV-1 - immunology</subject><subject>HIV-1 - isolation & purification</subject><subject>human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Immunization, Passive</subject><subject>Immunoglobulin Fab Fragments - immunology</subject><subject>Immunoglobulin G - immunology</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infants</subject><subject>Libraries</subject><subject>Male</subject><subject>Molecular immunology</subject><subject>Molecular Sequence Data</subject><subject>Monoclonal antibodies</subject><subject>Neutralization Tests</subject><subject>Reactivity</subject><subject>Recombinant Proteins - immunology</subject><subject>Vaccination</subject><subject>Viruses</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0s2LEzEUAPBBlLWunr0ozEHEw85uPuYjOdaytoW6FT_2OmYyLyVLJlknGbD-9aZ0qBQKlhwCeb_3CO-9JHmN0TXGpLzxUoOVcF3xipYFeZJMMOJFxgmiT5MJQrTMGKqK58kL7x8QijFOL5KLkU-Sn7dK6V2NkN7BEHph9B8RtLOpU-mXXnei36ZL74wI4Hdvi-V9htNmm4r0K0jXNdqKmLwYOmHTz846aZwVJp3aoBvXbl8mz5QwHl6N92Xy49Pt99kiW63ny9l0lckK5yHLqVBQMaoaShHjnJGcAS5bRStEcU4JbQWhvOFcCtwqYFBhDKKRomF52-T0Mnm_r_vYu18D-FB32kswRlhwg6-rkuUoR-S_EJclzjHjEV7t4UYYqLVVLrZHbsBC7JKzoHR8nuLYUIKK3QeyEzyeFjotT_kPRz6SAL_DRgze18tvd2fT9f3Z9OP8XMrmqyN6dYpKZwxsoI6DnK2P-M2ey95534OqH_ebVGNU79a2Hte2HvcwZrwdhzI0HbQH_y_-bowLL4VRvbBS-wOjlHNEisje7NmDD64_hAljRc4R_Qs5OvqO</recordid><startdate>19941111</startdate><enddate>19941111</enddate><creator>Burton, Dennis R.</creator><creator>Pyati, Jayashree</creator><creator>Koduri, Raju</creator><creator>Sharp, Stephen J.</creator><creator>Thornton, George B.</creator><creator>Paul W. 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Parren ; Lynette S. W. Sawyer ; Hendry, R. Michael ; Dunlop, Nancy ; Nara, Peter L. ; Lamacchia, Michael ; Garratty, Eileen ; Stiehm, E. 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Psychology</topic><topic>Fundamental immunology</topic><topic>HIV 1</topic><topic>HIV Antibodies - immunology</topic><topic>HIV Core Protein p24 - analysis</topic><topic>HIV Envelope Protein gp120 - immunology</topic><topic>HIV-1 - immunology</topic><topic>HIV-1 - isolation & purification</topic><topic>human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Immunization, Passive</topic><topic>Immunoglobulin Fab Fragments - immunology</topic><topic>Immunoglobulin G - immunology</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infants</topic><topic>Libraries</topic><topic>Male</topic><topic>Molecular immunology</topic><topic>Molecular Sequence Data</topic><topic>Monoclonal antibodies</topic><topic>Neutralization Tests</topic><topic>Reactivity</topic><topic>Recombinant Proteins - immunology</topic><topic>Vaccination</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burton, Dennis R.</creatorcontrib><creatorcontrib>Pyati, Jayashree</creatorcontrib><creatorcontrib>Koduri, Raju</creatorcontrib><creatorcontrib>Sharp, Stephen J.</creatorcontrib><creatorcontrib>Thornton, George B.</creatorcontrib><creatorcontrib>Paul W. 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H. I. Parren</au><au>Lynette S. W. Sawyer</au><au>Hendry, R. Michael</au><au>Dunlop, Nancy</au><au>Nara, Peter L.</au><au>Lamacchia, Michael</au><au>Garratty, Eileen</au><au>Stiehm, E. 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The recombinant antibody neutralized more than 75 percent of the primary isolates tested at concentrations that could be achieved by passive immunization, for example, to interrupt maternal-fetal transmission of virus. The broad specificity and efficacy of the antibody implies the conservation of a structural feature on gp120, which could be important in vaccine design.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>7973652</pmid><doi>10.1126/science.7973652</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1994-11, Vol.266 (5187), p.1024-1027 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76840402 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Acquired Immunodeficiency Syndrome - virology AIDS AIDS vaccines AIDS Vaccines - immunology AIDS/HIV Amino Acid Sequence Antibodies Antibodies, immunoglobulins Antibodies, Monoclonal - immunology Antibody Specificity Biological and medical sciences Enzyme linked immunosorbent assay Fundamental and applied biological sciences. Psychology Fundamental immunology HIV 1 HIV Antibodies - immunology HIV Core Protein p24 - analysis HIV Envelope Protein gp120 - immunology HIV-1 - immunology HIV-1 - isolation & purification human immunodeficiency virus 1 Humans Immunization, Passive Immunoglobulin Fab Fragments - immunology Immunoglobulin G - immunology Infant Infant, Newborn Infants Libraries Male Molecular immunology Molecular Sequence Data Monoclonal antibodies Neutralization Tests Reactivity Recombinant Proteins - immunology Vaccination Viruses |
| title | Efficient Neutralization of Primary Isolates of HIV-1 by a Recombinant Human Monoclonal Antibody |
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