Biological studies of a nitroso compound that releases nitric oxide upon illumination
2-Methyl-2-nitrosopropane (MNP) has long been known to undergo photochemical and thermal decomposition, generating di-tert-butyl nitroxide, in organic solvent. The present study was undertaken to demonstrate that MNP can be used as a caged-nitric oxide (NO), which can liberate NO upon illumination....
Gespeichert in:
| Veröffentlicht in: | Molecular pharmacology 1994-10, Vol.46 (4), p.709-715 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 715 |
|---|---|
| container_issue | 4 |
| container_start_page | 709 |
| container_title | Molecular pharmacology |
| container_volume | 46 |
| creator | Pou, S J Anderson, D E Surichamorn, W Keaton, L L Tod, M L |
| description | 2-Methyl-2-nitrosopropane (MNP) has long been known to undergo photochemical and thermal decomposition, generating di-tert-butyl
nitroxide, in organic solvent. The present study was undertaken to demonstrate that MNP can be used as a caged-nitric oxide
(NO), which can liberate NO upon illumination. Photolysis of MNP leads to the generation of tert-butyl radical and NO, as
detected by spin-trapping/ESR spectroscopy and by oxyhemoglobin/visible spectroscopy, respectively. Using soluble guanylate
cyclase in neuroblastoma N1E-115 cells as an NO target, we found that MNP in the presence of light caused a dose- and time-dependent
increase in cGMP. Finally, illumination of a solution of MNP was also found to induce relaxation of preconstricted isolated
rat pulmonary artery rings. These studies demonstrated that MNP can be useful biochemical research tool for delivering NO
in a controlled manner, by using light. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_76839365</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76839365</sourcerecordid><originalsourceid>FETCH-LOGICAL-h236t-2bfe9037875cbaeff6712ac61e9ffc4a445053367436c4392e08914fbe4991d63</originalsourceid><addsrcrecordid>eNotkE1LwzAch4Moc04_gpCL3gpJ89LmqMM3GHhx4C2k6T9rJG1qk6J-eze20-_wPPwOzxlaUlHSglBKz9GSkFIWtRKfl-gqpS9CKBc1WaBFpaQigizR9tHHEHfemoBTnlsPCUeHDR58nmKK2MZ-jPPQ4tyZjCcIYNLeOWBvcfz1LeB5jAP2Icy9H0z2cbhGF86EBDenXaHt89PH-rXYvL-8rR82RVcymYuycaAIq-pK2MaAc7KipbGSgnLOcsO5IIIxWXEmLWeqBFIryl0DXCnaSrZC98ffcYrfM6Sse58shGAGiHPSlayZYlLsxduTODc9tHqcfG-mP33qsOd3R975XffjJ9BjZ6be2EOcP82l5roiiv0DWkRoZA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76839365</pqid></control><display><type>article</type><title>Biological studies of a nitroso compound that releases nitric oxide upon illumination</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Pou, S J ; Anderson, D E ; Surichamorn, W ; Keaton, L L ; Tod, M L</creator><creatorcontrib>Pou, S J ; Anderson, D E ; Surichamorn, W ; Keaton, L L ; Tod, M L</creatorcontrib><description>2-Methyl-2-nitrosopropane (MNP) has long been known to undergo photochemical and thermal decomposition, generating di-tert-butyl
nitroxide, in organic solvent. The present study was undertaken to demonstrate that MNP can be used as a caged-nitric oxide
(NO), which can liberate NO upon illumination. Photolysis of MNP leads to the generation of tert-butyl radical and NO, as
detected by spin-trapping/ESR spectroscopy and by oxyhemoglobin/visible spectroscopy, respectively. Using soluble guanylate
cyclase in neuroblastoma N1E-115 cells as an NO target, we found that MNP in the presence of light caused a dose- and time-dependent
increase in cGMP. Finally, illumination of a solution of MNP was also found to induce relaxation of preconstricted isolated
rat pulmonary artery rings. These studies demonstrated that MNP can be useful biochemical research tool for delivering NO
in a controlled manner, by using light.</description><identifier>ISSN: 0026-895X</identifier><identifier>EISSN: 1521-0111</identifier><identifier>PMID: 7969050</identifier><language>eng</language><publisher>United States: American Society for Pharmacology and Experimental Therapeutics</publisher><subject>Animals ; Electron Spin Resonance Spectroscopy ; Guanylate Cyclase - metabolism ; Male ; Mice ; Muscle Relaxation - drug effects ; Neuroblastoma - enzymology ; Neuroblastoma - pathology ; Nitric Oxide - metabolism ; Nitroso Compounds - chemistry ; Nitroso Compounds - pharmacology ; Photochemistry ; Pulmonary Artery - drug effects ; Pulmonary Artery - metabolism ; Rats ; Rats, Sprague-Dawley ; Spin Labels ; Tumor Cells, Cultured</subject><ispartof>Molecular pharmacology, 1994-10, Vol.46 (4), p.709-715</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7969050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pou, S J</creatorcontrib><creatorcontrib>Anderson, D E</creatorcontrib><creatorcontrib>Surichamorn, W</creatorcontrib><creatorcontrib>Keaton, L L</creatorcontrib><creatorcontrib>Tod, M L</creatorcontrib><title>Biological studies of a nitroso compound that releases nitric oxide upon illumination</title><title>Molecular pharmacology</title><addtitle>Mol Pharmacol</addtitle><description>2-Methyl-2-nitrosopropane (MNP) has long been known to undergo photochemical and thermal decomposition, generating di-tert-butyl
nitroxide, in organic solvent. The present study was undertaken to demonstrate that MNP can be used as a caged-nitric oxide
(NO), which can liberate NO upon illumination. Photolysis of MNP leads to the generation of tert-butyl radical and NO, as
detected by spin-trapping/ESR spectroscopy and by oxyhemoglobin/visible spectroscopy, respectively. Using soluble guanylate
cyclase in neuroblastoma N1E-115 cells as an NO target, we found that MNP in the presence of light caused a dose- and time-dependent
increase in cGMP. Finally, illumination of a solution of MNP was also found to induce relaxation of preconstricted isolated
rat pulmonary artery rings. These studies demonstrated that MNP can be useful biochemical research tool for delivering NO
in a controlled manner, by using light.</description><subject>Animals</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Guanylate Cyclase - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Muscle Relaxation - drug effects</subject><subject>Neuroblastoma - enzymology</subject><subject>Neuroblastoma - pathology</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitroso Compounds - chemistry</subject><subject>Nitroso Compounds - pharmacology</subject><subject>Photochemistry</subject><subject>Pulmonary Artery - drug effects</subject><subject>Pulmonary Artery - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spin Labels</subject><subject>Tumor Cells, Cultured</subject><issn>0026-895X</issn><issn>1521-0111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkE1LwzAch4Moc04_gpCL3gpJ89LmqMM3GHhx4C2k6T9rJG1qk6J-eze20-_wPPwOzxlaUlHSglBKz9GSkFIWtRKfl-gqpS9CKBc1WaBFpaQigizR9tHHEHfemoBTnlsPCUeHDR58nmKK2MZ-jPPQ4tyZjCcIYNLeOWBvcfz1LeB5jAP2Icy9H0z2cbhGF86EBDenXaHt89PH-rXYvL-8rR82RVcymYuycaAIq-pK2MaAc7KipbGSgnLOcsO5IIIxWXEmLWeqBFIryl0DXCnaSrZC98ffcYrfM6Sse58shGAGiHPSlayZYlLsxduTODc9tHqcfG-mP33qsOd3R975XffjJ9BjZ6be2EOcP82l5roiiv0DWkRoZA</recordid><startdate>19941001</startdate><enddate>19941001</enddate><creator>Pou, S J</creator><creator>Anderson, D E</creator><creator>Surichamorn, W</creator><creator>Keaton, L L</creator><creator>Tod, M L</creator><general>American Society for Pharmacology and Experimental Therapeutics</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19941001</creationdate><title>Biological studies of a nitroso compound that releases nitric oxide upon illumination</title><author>Pou, S J ; Anderson, D E ; Surichamorn, W ; Keaton, L L ; Tod, M L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h236t-2bfe9037875cbaeff6712ac61e9ffc4a445053367436c4392e08914fbe4991d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Guanylate Cyclase - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Muscle Relaxation - drug effects</topic><topic>Neuroblastoma - enzymology</topic><topic>Neuroblastoma - pathology</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitroso Compounds - chemistry</topic><topic>Nitroso Compounds - pharmacology</topic><topic>Photochemistry</topic><topic>Pulmonary Artery - drug effects</topic><topic>Pulmonary Artery - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spin Labels</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pou, S J</creatorcontrib><creatorcontrib>Anderson, D E</creatorcontrib><creatorcontrib>Surichamorn, W</creatorcontrib><creatorcontrib>Keaton, L L</creatorcontrib><creatorcontrib>Tod, M L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pou, S J</au><au>Anderson, D E</au><au>Surichamorn, W</au><au>Keaton, L L</au><au>Tod, M L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological studies of a nitroso compound that releases nitric oxide upon illumination</atitle><jtitle>Molecular pharmacology</jtitle><addtitle>Mol Pharmacol</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>46</volume><issue>4</issue><spage>709</spage><epage>715</epage><pages>709-715</pages><issn>0026-895X</issn><eissn>1521-0111</eissn><abstract>2-Methyl-2-nitrosopropane (MNP) has long been known to undergo photochemical and thermal decomposition, generating di-tert-butyl
nitroxide, in organic solvent. The present study was undertaken to demonstrate that MNP can be used as a caged-nitric oxide
(NO), which can liberate NO upon illumination. Photolysis of MNP leads to the generation of tert-butyl radical and NO, as
detected by spin-trapping/ESR spectroscopy and by oxyhemoglobin/visible spectroscopy, respectively. Using soluble guanylate
cyclase in neuroblastoma N1E-115 cells as an NO target, we found that MNP in the presence of light caused a dose- and time-dependent
increase in cGMP. Finally, illumination of a solution of MNP was also found to induce relaxation of preconstricted isolated
rat pulmonary artery rings. These studies demonstrated that MNP can be useful biochemical research tool for delivering NO
in a controlled manner, by using light.</abstract><cop>United States</cop><pub>American Society for Pharmacology and Experimental Therapeutics</pub><pmid>7969050</pmid><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-895X |
| ispartof | Molecular pharmacology, 1994-10, Vol.46 (4), p.709-715 |
| issn | 0026-895X 1521-0111 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76839365 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Electron Spin Resonance Spectroscopy Guanylate Cyclase - metabolism Male Mice Muscle Relaxation - drug effects Neuroblastoma - enzymology Neuroblastoma - pathology Nitric Oxide - metabolism Nitroso Compounds - chemistry Nitroso Compounds - pharmacology Photochemistry Pulmonary Artery - drug effects Pulmonary Artery - metabolism Rats Rats, Sprague-Dawley Spin Labels Tumor Cells, Cultured |
| title | Biological studies of a nitroso compound that releases nitric oxide upon illumination |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T03%3A34%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biological%20studies%20of%20a%20nitroso%20compound%20that%20releases%20nitric%20oxide%20upon%20illumination&rft.jtitle=Molecular%20pharmacology&rft.au=Pou,%20S%20J&rft.date=1994-10-01&rft.volume=46&rft.issue=4&rft.spage=709&rft.epage=715&rft.pages=709-715&rft.issn=0026-895X&rft.eissn=1521-0111&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E76839365%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76839365&rft_id=info:pmid/7969050&rfr_iscdi=true |