Successful Donor Cell Engraftment in a Recipient of Bone Marrow from a Cadaveric Donor
A 12-year-old male with acute lymphocytic leukemia received donor bone marrow from his histocompatible father whose marrow was harvested 40 minutes postmortem after he suffered a myocardial infarction. The marrow was stored in liquid nitrogen for 17 days prior to infusion into the recipient. Trypan...
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| Veröffentlicht in: | Blood 1986-06, Vol.67 (6), p.1655-1660 |
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| container_issue | 6 |
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| container_title | Blood |
| container_volume | 67 |
| creator | Blazar, Bruce R. Lasky, Larry C. Perentesis, John P. Watson, Kathleen V. Steinberg, Stephen E. Filipovich, Alexandra H. Orr, Harry T. Ramsay, Norma K.C. |
| description | A 12-year-old male with acute lymphocytic leukemia received donor bone marrow from his histocompatible father whose marrow was harvested 40 minutes postmortem after he suffered a myocardial infarction. The marrow was stored in liquid nitrogen for 17 days prior to infusion into the recipient. Trypan blue viability was >99% for the fresh marrow. Progenitor cell assays revealed that 20% of the CFU-MIX, 16% of the BFU-E, 10% of the CFU-E, and 17% of the CFU-GM were spared during the cryopreserva-tion period. Posttransplantation, the recipient had a leuko- cyte count >103/µL by day 26. Southern blotting analysis documented the donor origin of the peripheral blood mononuclear cells and granulocytes isolated 46 days posttrans-plantation. Unfortunately, the patient died of complications relating to graft-v-host disease 67 days following transplantation. This case demonstrates the feasibility of cadaveric marrow as a source of donor cells and is the first reported case of documented leukocyte engraftment in a recipient of cadaveric marrow. |
| doi_str_mv | 10.1182/blood.V67.6.1655.1655 |
| format | Article |
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The marrow was stored in liquid nitrogen for 17 days prior to infusion into the recipient. Trypan blue viability was >99% for the fresh marrow. Progenitor cell assays revealed that 20% of the CFU-MIX, 16% of the BFU-E, 10% of the CFU-E, and 17% of the CFU-GM were spared during the cryopreserva-tion period. Posttransplantation, the recipient had a leuko- cyte count >103/µL by day 26. Southern blotting analysis documented the donor origin of the peripheral blood mononuclear cells and granulocytes isolated 46 days posttrans-plantation. Unfortunately, the patient died of complications relating to graft-v-host disease 67 days following transplantation. This case demonstrates the feasibility of cadaveric marrow as a source of donor cells and is the first reported case of documented leukocyte engraftment in a recipient of cadaveric marrow.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V67.6.1655.1655</identifier><identifier>PMID: 3518833</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Bone Marrow Transplantation ; Bone marrow, stem cells transplantation. Graft versus host reaction ; Cadaver ; Child ; Erythrocytes - analysis ; Genotype ; Graft vs Host Disease ; Humans ; Leukemia, Lymphoid - therapy ; Male ; Medical sciences ; Phenotype ; Polymorphism, Genetic ; Stem Cells - cytology ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Blood, 1986-06, Vol.67 (6), p.1655-1660</ispartof><rights>1986 American Society of Hematology</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-486e471addddfda4d75fba4f83120f645b44ce906e4072cb01df88fde31b99f33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8768861$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3518833$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blazar, Bruce R.</creatorcontrib><creatorcontrib>Lasky, Larry C.</creatorcontrib><creatorcontrib>Perentesis, John P.</creatorcontrib><creatorcontrib>Watson, Kathleen V.</creatorcontrib><creatorcontrib>Steinberg, Stephen E.</creatorcontrib><creatorcontrib>Filipovich, Alexandra H.</creatorcontrib><creatorcontrib>Orr, Harry T.</creatorcontrib><creatorcontrib>Ramsay, Norma K.C.</creatorcontrib><title>Successful Donor Cell Engraftment in a Recipient of Bone Marrow from a Cadaveric Donor</title><title>Blood</title><addtitle>Blood</addtitle><description>A 12-year-old male with acute lymphocytic leukemia received donor bone marrow from his histocompatible father whose marrow was harvested 40 minutes postmortem after he suffered a myocardial infarction. The marrow was stored in liquid nitrogen for 17 days prior to infusion into the recipient. Trypan blue viability was >99% for the fresh marrow. Progenitor cell assays revealed that 20% of the CFU-MIX, 16% of the BFU-E, 10% of the CFU-E, and 17% of the CFU-GM were spared during the cryopreserva-tion period. Posttransplantation, the recipient had a leuko- cyte count >103/µL by day 26. Southern blotting analysis documented the donor origin of the peripheral blood mononuclear cells and granulocytes isolated 46 days posttrans-plantation. Unfortunately, the patient died of complications relating to graft-v-host disease 67 days following transplantation. This case demonstrates the feasibility of cadaveric marrow as a source of donor cells and is the first reported case of documented leukocyte engraftment in a recipient of cadaveric marrow.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Transplantation</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>Cadaver</subject><subject>Child</subject><subject>Erythrocytes - analysis</subject><subject>Genotype</subject><subject>Graft vs Host Disease</subject><subject>Humans</subject><subject>Leukemia, Lymphoid - therapy</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Phenotype</subject><subject>Polymorphism, Genetic</subject><subject>Stem Cells - cytology</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v3CAQhlHUKN1s8hMicah68xYMxvhUtU7SRkoUKV9XhGGoqGyzBXuj_vuyH9pr5zAj9L4zzDwIXVGyolSWX7o-BLt6E_VKrKioql06QQtalbIgpCQf0IIQIgre1PQjOk_pNyGUs7I6Q2esolIytkBvz7MxkJKbe3wdxhBxC32Pb8ZfUbtpgHHCfsQaP4Hxa799Boe_hxHwg44xvGMXw5D1Vlu9gejNfsoFOnW6T3B5qEv0envz0v4s7h9_3LXf7gvDhJwKLgXwmmqbw1nNbV25TnMnGS2JE7zqODfQkOwidWk6Qq2T0llgtGsax9gSfd7PXcfwZ4Y0qcEnky_QI4Q5qVpIJpt89RJVe6OJIaUITq2jH3T8qyhRW55qx1NlnkqoLcpdyn1Xhw_mbgB77DoAzPqng66T0b2LejQ-HW0yLyAFzbavextkGBsPUSWTaRqwPoKZlA3-P4v8A-1ElMw</recordid><startdate>19860601</startdate><enddate>19860601</enddate><creator>Blazar, Bruce R.</creator><creator>Lasky, Larry C.</creator><creator>Perentesis, John P.</creator><creator>Watson, Kathleen V.</creator><creator>Steinberg, Stephen E.</creator><creator>Filipovich, Alexandra H.</creator><creator>Orr, Harry T.</creator><creator>Ramsay, Norma K.C.</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860601</creationdate><title>Successful Donor Cell Engraftment in a Recipient of Bone Marrow from a Cadaveric Donor</title><author>Blazar, Bruce R. ; Lasky, Larry C. ; Perentesis, John P. ; Watson, Kathleen V. ; Steinberg, Stephen E. ; Filipovich, Alexandra H. ; Orr, Harry T. ; Ramsay, Norma K.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-486e471addddfda4d75fba4f83120f645b44ce906e4072cb01df88fde31b99f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Transplantation</topic><topic>Bone marrow, stem cells transplantation. Graft versus host reaction</topic><topic>Cadaver</topic><topic>Child</topic><topic>Erythrocytes - analysis</topic><topic>Genotype</topic><topic>Graft vs Host Disease</topic><topic>Humans</topic><topic>Leukemia, Lymphoid - therapy</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Phenotype</topic><topic>Polymorphism, Genetic</topic><topic>Stem Cells - cytology</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blazar, Bruce R.</creatorcontrib><creatorcontrib>Lasky, Larry C.</creatorcontrib><creatorcontrib>Perentesis, John P.</creatorcontrib><creatorcontrib>Watson, Kathleen V.</creatorcontrib><creatorcontrib>Steinberg, Stephen E.</creatorcontrib><creatorcontrib>Filipovich, Alexandra H.</creatorcontrib><creatorcontrib>Orr, Harry T.</creatorcontrib><creatorcontrib>Ramsay, Norma K.C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blazar, Bruce R.</au><au>Lasky, Larry C.</au><au>Perentesis, John P.</au><au>Watson, Kathleen V.</au><au>Steinberg, Stephen E.</au><au>Filipovich, Alexandra H.</au><au>Orr, Harry T.</au><au>Ramsay, Norma K.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Successful Donor Cell Engraftment in a Recipient of Bone Marrow from a Cadaveric Donor</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1986-06-01</date><risdate>1986</risdate><volume>67</volume><issue>6</issue><spage>1655</spage><epage>1660</epage><pages>1655-1660</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>A 12-year-old male with acute lymphocytic leukemia received donor bone marrow from his histocompatible father whose marrow was harvested 40 minutes postmortem after he suffered a myocardial infarction. The marrow was stored in liquid nitrogen for 17 days prior to infusion into the recipient. Trypan blue viability was >99% for the fresh marrow. Progenitor cell assays revealed that 20% of the CFU-MIX, 16% of the BFU-E, 10% of the CFU-E, and 17% of the CFU-GM were spared during the cryopreserva-tion period. Posttransplantation, the recipient had a leuko- cyte count >103/µL by day 26. Southern blotting analysis documented the donor origin of the peripheral blood mononuclear cells and granulocytes isolated 46 days posttrans-plantation. Unfortunately, the patient died of complications relating to graft-v-host disease 67 days following transplantation. This case demonstrates the feasibility of cadaveric marrow as a source of donor cells and is the first reported case of documented leukocyte engraftment in a recipient of cadaveric marrow.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>3518833</pmid><doi>10.1182/blood.V67.6.1655.1655</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0006-4971 |
| ispartof | Blood, 1986-06, Vol.67 (6), p.1655-1660 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_76838914 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Bone Marrow Transplantation Bone marrow, stem cells transplantation. Graft versus host reaction Cadaver Child Erythrocytes - analysis Genotype Graft vs Host Disease Humans Leukemia, Lymphoid - therapy Male Medical sciences Phenotype Polymorphism, Genetic Stem Cells - cytology Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Successful Donor Cell Engraftment in a Recipient of Bone Marrow from a Cadaveric Donor |
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