The scanning force microscopy of DNA in air and in n-propanol using new spreading agents
We present recent advances in DNA specimen preparation technique for scanning force microscopy (SFM) based on spreading on mica in the presence of cationic and non-ionic detergents. Reproducible DNA imaging in air and in n-propanol has been achieved in the presence of the non-ionic detergent 2,4,6-t...
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| Veröffentlicht in: | FEBS letters 1994-11, Vol.355 (1), p.91-95 |
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| creator | Schaper, Achim Starink, J.P.Pascual Jovin, Thomas M. |
| description | We present recent advances in DNA specimen preparation technique for scanning force microscopy (SFM) based on spreading on mica in the presence of cationic and non-ionic detergents. Reproducible DNA imaging in air and in
n-propanol has been achieved in the presence of the non-ionic detergent 2,4,6-tris(dimethylaminomethyl) phenol (DMP-30) or the cationic detergent cetylpyridinium chloride (CP) in a microdrop containing nanograms of DNA. In an alternative procedure, a microdrop of detergent is applied to the surface just prior to the DNA. Quantitative image analysis yields as the apparent molecular dimensions of the DNA a width of ∼ 7 nm and a height of ∼ 0.7 nm, and delineates the problems of DNA metrology by SFM. |
| doi_str_mv | 10.1016/0014-5793(94)01166-4 |
| format | Article |
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n-propanol has been achieved in the presence of the non-ionic detergent 2,4,6-tris(dimethylaminomethyl) phenol (DMP-30) or the cationic detergent cetylpyridinium chloride (CP) in a microdrop containing nanograms of DNA. In an alternative procedure, a microdrop of detergent is applied to the surface just prior to the DNA. Quantitative image analysis yields as the apparent molecular dimensions of the DNA a width of ∼ 7 nm and a height of ∼ 0.7 nm, and delineates the problems of DNA metrology by SFM.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(94)01166-4</identifier><identifier>PMID: 7957969</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>1-Propanol ; 2,4,6-Tris(dimethylaminomethyl)phenol ; Atomic force microscopy ; Benzalkonium Compounds ; Benzylalkonium chloride ; Cetylpyridinium ; Cetylpyridinium chloride ; DNA ; DNA - chemistry ; DNA - ultrastructure ; Image Processing, Computer-Assisted ; Microscopy - methods ; Microscopy, Atomic Force - methods ; Nucleic Acid Conformation ; Phenols ; Scanning tunneling microscopy ; Software ; Specimen Handling - methods</subject><ispartof>FEBS letters, 1994-11, Vol.355 (1), p.91-95</ispartof><rights>1994</rights><rights>FEBS Letters 355 (1994) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4864-f200607c19f612eb3ac5ddf44b9a440347b9d3d2b020321a527d17b7d102e9ac3</citedby><cites>FETCH-LOGICAL-c4864-f200607c19f612eb3ac5ddf44b9a440347b9d3d2b020321a527d17b7d102e9ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(94)01166-4$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7957969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schaper, Achim</creatorcontrib><creatorcontrib>Starink, J.P.Pascual</creatorcontrib><creatorcontrib>Jovin, Thomas M.</creatorcontrib><title>The scanning force microscopy of DNA in air and in n-propanol using new spreading agents</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We present recent advances in DNA specimen preparation technique for scanning force microscopy (SFM) based on spreading on mica in the presence of cationic and non-ionic detergents. Reproducible DNA imaging in air and in
n-propanol has been achieved in the presence of the non-ionic detergent 2,4,6-tris(dimethylaminomethyl) phenol (DMP-30) or the cationic detergent cetylpyridinium chloride (CP) in a microdrop containing nanograms of DNA. In an alternative procedure, a microdrop of detergent is applied to the surface just prior to the DNA. Quantitative image analysis yields as the apparent molecular dimensions of the DNA a width of ∼ 7 nm and a height of ∼ 0.7 nm, and delineates the problems of DNA metrology by SFM.</description><subject>1-Propanol</subject><subject>2,4,6-Tris(dimethylaminomethyl)phenol</subject><subject>Atomic force microscopy</subject><subject>Benzalkonium Compounds</subject><subject>Benzylalkonium chloride</subject><subject>Cetylpyridinium</subject><subject>Cetylpyridinium chloride</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA - ultrastructure</subject><subject>Image Processing, Computer-Assisted</subject><subject>Microscopy - methods</subject><subject>Microscopy, Atomic Force - methods</subject><subject>Nucleic Acid Conformation</subject><subject>Phenols</subject><subject>Scanning tunneling microscopy</subject><subject>Software</subject><subject>Specimen Handling - methods</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUMFO3DAQtVARXSh_QCWfKnoI2M6sHV8qAWUpEqIXKvVmOfYEXGWd1N4F7d8TsyuOVS-25715b8aPkBPOzjjj8pwxDtVc6fpUw1fGuZQV7JEZb1Rd1SCbD2T23vKRHOb8h011w_UBOVB6QqWekd8PT0izszGG-Ei7ITmky-DSkN0wbujQ0e_3FzREakOiNvryjNWYhtHGoafrXGQRX2geE1pfKvuIcZU_kf3O9hmPd_cR-bW4frj6Ud39vLm9urirHDQSqk4wJplyXHeSC2xr6-bedwCttgCsBtVqX3vRMsFqwe1cKM9VOx1MoLauPiJftr7TTn_XmFdmGbLDvrcRh3U2SjYCBKipEbaN5XM5YWfGFJY2bQxnpgRqSlqmpGU0mLdADUyyzzv_dbtE_y7aJTjxiy3_Enrc_JenWVxfikIUXMMbWgZ92xrhlNZzwGSyCxgd-pDQrYwfwr83fQUzbZb1</recordid><startdate>19941121</startdate><enddate>19941121</enddate><creator>Schaper, Achim</creator><creator>Starink, J.P.Pascual</creator><creator>Jovin, Thomas M.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941121</creationdate><title>The scanning force microscopy of DNA in air and in n-propanol using new spreading agents</title><author>Schaper, Achim ; Starink, J.P.Pascual ; Jovin, Thomas M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4864-f200607c19f612eb3ac5ddf44b9a440347b9d3d2b020321a527d17b7d102e9ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>1-Propanol</topic><topic>2,4,6-Tris(dimethylaminomethyl)phenol</topic><topic>Atomic force microscopy</topic><topic>Benzalkonium Compounds</topic><topic>Benzylalkonium chloride</topic><topic>Cetylpyridinium</topic><topic>Cetylpyridinium chloride</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>DNA - ultrastructure</topic><topic>Image Processing, Computer-Assisted</topic><topic>Microscopy - methods</topic><topic>Microscopy, Atomic Force - methods</topic><topic>Nucleic Acid Conformation</topic><topic>Phenols</topic><topic>Scanning tunneling microscopy</topic><topic>Software</topic><topic>Specimen Handling - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schaper, Achim</creatorcontrib><creatorcontrib>Starink, J.P.Pascual</creatorcontrib><creatorcontrib>Jovin, Thomas M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schaper, Achim</au><au>Starink, J.P.Pascual</au><au>Jovin, Thomas M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The scanning force microscopy of DNA in air and in n-propanol using new spreading agents</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1994-11-21</date><risdate>1994</risdate><volume>355</volume><issue>1</issue><spage>91</spage><epage>95</epage><pages>91-95</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We present recent advances in DNA specimen preparation technique for scanning force microscopy (SFM) based on spreading on mica in the presence of cationic and non-ionic detergents. Reproducible DNA imaging in air and in
n-propanol has been achieved in the presence of the non-ionic detergent 2,4,6-tris(dimethylaminomethyl) phenol (DMP-30) or the cationic detergent cetylpyridinium chloride (CP) in a microdrop containing nanograms of DNA. In an alternative procedure, a microdrop of detergent is applied to the surface just prior to the DNA. Quantitative image analysis yields as the apparent molecular dimensions of the DNA a width of ∼ 7 nm and a height of ∼ 0.7 nm, and delineates the problems of DNA metrology by SFM.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>7957969</pmid><doi>10.1016/0014-5793(94)01166-4</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1994-11, Vol.355 (1), p.91-95 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 1-Propanol 2,4,6-Tris(dimethylaminomethyl)phenol Atomic force microscopy Benzalkonium Compounds Benzylalkonium chloride Cetylpyridinium Cetylpyridinium chloride DNA DNA - chemistry DNA - ultrastructure Image Processing, Computer-Assisted Microscopy - methods Microscopy, Atomic Force - methods Nucleic Acid Conformation Phenols Scanning tunneling microscopy Software Specimen Handling - methods |
| title | The scanning force microscopy of DNA in air and in n-propanol using new spreading agents |
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