Cloning, Sequence, and Expression of Bovine Interleukin 2

Interleukin 2 (IL-2) cDNA clones have been isolated from both human and murine sources. We report here the isolation of a cDNA clone encoding bovine IL-2. This was accomplished by screening a cDNA library constructed from lectin-stimulated bovine lymph node cells, using a human IL-2 probe. Bovine IL...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1986-05, Vol.83 (10), p.3223-3227
Hauptverfasser:	Cerretti, Douglas Pat, McKereghan, Kate, Larsen, Alf, Cantrell, Michael A., Anderson, Dirk, Gillis, Steven, Cosman, David, Baker, Paul E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Animals Base Sequence Biological and medical sciences Biotechnology Cattle Cloning, Molecular Complementary DNA DNA DNA - genetics Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes Genetic engineering Genetic technics Interleukin-2 - biosynthesis Interleukin-2 - genetics Messenger RNA Methods. Procedures. Technologies Mice Molecular cloning Molecular weight Plasmids Recombinant Proteins - biosynthesis Recombinant Proteins - genetics RNA RNA, Messenger - genetics T lymphocytes Ungulates
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Zusammenfassung:	Interleukin 2 (IL-2) cDNA clones have been isolated from both human and murine sources. We report here the isolation of a cDNA clone encoding bovine IL-2. This was accomplished by screening a cDNA library constructed from lectin-stimulated bovine lymph node cells, using a human IL-2 probe. Bovine IL-2 is composed of 155 amino acids and has a predicted molecular weight of 19,555. Alignment of the amino acid sequence with human IL-2 indicates that mature bovine IL-2 is composed of 135 amino acids and has a predicted molecular weight of 15,452. It has an amino acid homology of 65% with human IL-2 and 50% with murine IL-2. Bovine IL-2 is unique among IL-2 homologs in that it has a single N-linked glycosylation site. Biologically active bovine IL-2 was synthesized in an Escherichia coli expression system.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.83.10.3223