Hypertriglyceridemic Serum, Very Low Density Lipoprotein, And Iron Enhance Mycobacterium Avium Replication In Human Macrophages
The growth of Mycobacterium avium 7497, serovar 4, in cultured human macrophages is enhanced by Fe3+ and serum lipids over 7 days. Iron (1–80 µg/mL) added to macrophages cultured in normal serum resulted in 10-fold increases in growth. If iron-supplemented macrophages were cultured in serum from hyp...
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| Veröffentlicht in: | The Journal of infectious diseases 1994-11, Vol.170 (5), p.1248-1255 |
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| creator | Douvas, George S. May, Mary H. Richard Pearson, J. Lam, Elaine Miller, Leann Tsuchida, Norma |
| description | The growth of Mycobacterium avium 7497, serovar 4, in cultured human macrophages is enhanced by Fe3+ and serum lipids over 7 days. Iron (1–80 µg/mL) added to macrophages cultured in normal serum resulted in 10-fold increases in growth. If iron-supplemented macrophages were cultured in serum from hypertriglyceridemic donors after infection, M. avium growth increased 103 - to 104 -fold. Without macrophages, differences in bacterial growth between sera were not seen. Removal of very low density lipoprotein (VLDL) eliminated the differences between sera. Isolated VLDL from hyperlipidemic serum resulted in 105 -fold increases in growth over that seen with VLDL from normal sera. Accelerated M. avium growth in macrophages cultured with hyperlipidemic serum was partly inhibited by the addition of superoxide dismutase (1000 IU/mL). Results suggest that iron stimulates O2 -induced oxidation of VLDL and its subsequent accumulation in macrophages. The resultant iron- and lipid-laden cells become excellent hosts for mycobacterial growth. |
| doi_str_mv | 10.1093/infdis/170.5.1248 |
| format | Article |
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Iron (1–80 µg/mL) added to macrophages cultured in normal serum resulted in 10-fold increases in growth. If iron-supplemented macrophages were cultured in serum from hypertriglyceridemic donors after infection, M. avium growth increased 103 - to 104 -fold. Without macrophages, differences in bacterial growth between sera were not seen. Removal of very low density lipoprotein (VLDL) eliminated the differences between sera. Isolated VLDL from hyperlipidemic serum resulted in 105 -fold increases in growth over that seen with VLDL from normal sera. Accelerated M. avium growth in macrophages cultured with hyperlipidemic serum was partly inhibited by the addition of superoxide dismutase (1000 IU/mL). Results suggest that iron stimulates O2 -induced oxidation of VLDL and its subsequent accumulation in macrophages. 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Iron (1–80 µg/mL) added to macrophages cultured in normal serum resulted in 10-fold increases in growth. If iron-supplemented macrophages were cultured in serum from hypertriglyceridemic donors after infection, M. avium growth increased 103 - to 104 -fold. Without macrophages, differences in bacterial growth between sera were not seen. Removal of very low density lipoprotein (VLDL) eliminated the differences between sera. Isolated VLDL from hyperlipidemic serum resulted in 105 -fold increases in growth over that seen with VLDL from normal sera. Accelerated M. avium growth in macrophages cultured with hyperlipidemic serum was partly inhibited by the addition of superoxide dismutase (1000 IU/mL). Results suggest that iron stimulates O2 -induced oxidation of VLDL and its subsequent accumulation in macrophages. The resultant iron- and lipid-laden cells become excellent hosts for mycobacterial growth.</description><subject>Acquired Immunodeficiency Syndrome - blood</subject><subject>Adult</subject><subject>AIDS</subject><subject>AIDS/HIV</subject><subject>Cholesterols</subject><subject>Female</subject><subject>Humans</subject><subject>Hypertriglyceridemia - blood</subject><subject>Infections</subject><subject>Iron</subject><subject>Iron - pharmacology</subject><subject>Lipids</subject><subject>Lipoproteins</subject><subject>Lipoproteins, VLDL - blood</subject><subject>Lipoproteins, VLDL - physiology</subject><subject>Macrophages</subject><subject>Macrophages - microbiology</subject><subject>Major Articles</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium avium - growth & development</subject><subject>Superoxide Dismutase - pharmacology</subject><subject>Superoxides</subject><subject>Triglycerides</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE9v0zAYhy0EGt3gA3BA8onT0vlPbMfHagxSqR2iDIS4WI7jbB6JE-xkkBNfHVetysW29Pzen14_ALzBaImRpFfON7WLV1igJVtikhfPwAIzKjLOMX0OFggRkuFCypfgPMZHhFBOuTgDZ0JyKghagL_lPNgwBnffzsYGV9vOGfjFhqm7hN9smOGm_w3fWx_dmN5u6IfQj9b5S7jyNVyH3sMb_6C9sXA7m77SZkw1UwdXT_tzZ4fWGT26lFt7WE6d9nCrTeiHB31v4yvwotFttK-P9wX4-uHm7rrMNp8-rq9Xm8xQxsesMA1pbFMYXuTGMMIrXTQ0x1giLqjR1phaIJojw5uqLnTNBKm5lrUUjElU0Qvw7tCb1v812TiqzkVj21Z7209RCV5gSYhMQXwIphVjDLZRQ3CdDrPCSO2lq4N0laQrpvbS08zbY_lUdbY-TRwt_-ePcezDCVOEKWU5Tzw7cBdH--fEdfip0u8EU-X3H6q8-3y7297uFKL_AHKOmkQ</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Douvas, George S.</creator><creator>May, Mary H.</creator><creator>Richard Pearson, J.</creator><creator>Lam, Elaine</creator><creator>Miller, Leann</creator><creator>Tsuchida, Norma</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941101</creationdate><title>Hypertriglyceridemic Serum, Very Low Density Lipoprotein, And Iron Enhance Mycobacterium Avium Replication In Human Macrophages</title><author>Douvas, George S. ; May, Mary H. ; Richard Pearson, J. ; Lam, Elaine ; Miller, Leann ; Tsuchida, Norma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-8cf2fef8c684cc526ba8f341190673caeccd70340c6fbd8ad572d6a9d975590b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acquired Immunodeficiency Syndrome - blood</topic><topic>Adult</topic><topic>AIDS</topic><topic>AIDS/HIV</topic><topic>Cholesterols</topic><topic>Female</topic><topic>Humans</topic><topic>Hypertriglyceridemia - blood</topic><topic>Infections</topic><topic>Iron</topic><topic>Iron - pharmacology</topic><topic>Lipids</topic><topic>Lipoproteins</topic><topic>Lipoproteins, VLDL - blood</topic><topic>Lipoproteins, VLDL - physiology</topic><topic>Macrophages</topic><topic>Macrophages - microbiology</topic><topic>Major Articles</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium avium - growth & development</topic><topic>Superoxide Dismutase - pharmacology</topic><topic>Superoxides</topic><topic>Triglycerides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Douvas, George S.</creatorcontrib><creatorcontrib>May, Mary H.</creatorcontrib><creatorcontrib>Richard Pearson, J.</creatorcontrib><creatorcontrib>Lam, Elaine</creatorcontrib><creatorcontrib>Miller, Leann</creatorcontrib><creatorcontrib>Tsuchida, Norma</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Douvas, George S.</au><au>May, Mary H.</au><au>Richard Pearson, J.</au><au>Lam, Elaine</au><au>Miller, Leann</au><au>Tsuchida, Norma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypertriglyceridemic Serum, Very Low Density Lipoprotein, And Iron Enhance Mycobacterium Avium Replication In Human Macrophages</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>170</volume><issue>5</issue><spage>1248</spage><epage>1255</epage><pages>1248-1255</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><abstract>The growth of Mycobacterium avium 7497, serovar 4, in cultured human macrophages is enhanced by Fe3+ and serum lipids over 7 days. Iron (1–80 µg/mL) added to macrophages cultured in normal serum resulted in 10-fold increases in growth. If iron-supplemented macrophages were cultured in serum from hypertriglyceridemic donors after infection, M. avium growth increased 103 - to 104 -fold. Without macrophages, differences in bacterial growth between sera were not seen. Removal of very low density lipoprotein (VLDL) eliminated the differences between sera. Isolated VLDL from hyperlipidemic serum resulted in 105 -fold increases in growth over that seen with VLDL from normal sera. Accelerated M. avium growth in macrophages cultured with hyperlipidemic serum was partly inhibited by the addition of superoxide dismutase (1000 IU/mL). Results suggest that iron stimulates O2 -induced oxidation of VLDL and its subsequent accumulation in macrophages. The resultant iron- and lipid-laden cells become excellent hosts for mycobacterial growth.</abstract><cop>United States</cop><pub>The University of Chicago Press</pub><pmid>7963720</pmid><doi>10.1093/infdis/170.5.1248</doi><tpages>8</tpages></addata></record> |
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| subjects | Acquired Immunodeficiency Syndrome - blood Adult AIDS AIDS/HIV Cholesterols Female Humans Hypertriglyceridemia - blood Infections Iron Iron - pharmacology Lipids Lipoproteins Lipoproteins, VLDL - blood Lipoproteins, VLDL - physiology Macrophages Macrophages - microbiology Major Articles Male Middle Aged Mycobacterium avium Mycobacterium avium - growth & development Superoxide Dismutase - pharmacology Superoxides Triglycerides |
| title | Hypertriglyceridemic Serum, Very Low Density Lipoprotein, And Iron Enhance Mycobacterium Avium Replication In Human Macrophages |
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