Cyclic GMP-sensitive conductance of retinal rods consists of aqueous pores
The surface membrane of retinal rod and cone outer segments contains a cation-selective conductance which is activated by 3′,5′-cyclic guanosine monophosphate (cGMP) 1–4 . Reduction of this conductance by a light-induced decrease in the cytoplasmic concentration of cGMP appears to generate the elect...
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| Veröffentlicht in: | Nature (London) 1986-05, Vol.321 (6065), p.70-72 |
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| creator | Zimmerman, A. L Baylor, D. A |
| description | The surface membrane of retinal rod and cone outer segments contains a cation-selective conductance which is activated by 3′,5′-cyclic guanosine monophosphate (cGMP)
1–4
. Reduction of this conductance by a light-induced decrease in the cytoplasmic concentration of cGMP appears to generate the electrical response to light (reviewed in refs 5, 6), but little is known about the molecular nature of the conductance. The estimated unitary conductance is so small
1,7–9
that ion transport might occur via either a carrier or a pore mechanism. Here we report recordings of cGMP-activated single-channel currents from excised rod outer segment patches bathed in solutions low in divalent cations. Two elementary conductances, of ∼24 and 8 pS, were observed. These conductances are too large to be accounted for by carrier transport, indicating that the cGMP-activated conductance consists of aqueous pores. The dependence of the channel activation on the concentration of cGMP suggests that opening of the pore is triggered by cooperative binding of at least three cGMP molecules. |
| doi_str_mv | 10.1038/321070a0 |
| format | Article |
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1–4
. Reduction of this conductance by a light-induced decrease in the cytoplasmic concentration of cGMP appears to generate the electrical response to light (reviewed in refs 5, 6), but little is known about the molecular nature of the conductance. The estimated unitary conductance is so small
1,7–9
that ion transport might occur via either a carrier or a pore mechanism. Here we report recordings of cGMP-activated single-channel currents from excised rod outer segment patches bathed in solutions low in divalent cations. Two elementary conductances, of ∼24 and 8 pS, were observed. These conductances are too large to be accounted for by carrier transport, indicating that the cGMP-activated conductance consists of aqueous pores. The dependence of the channel activation on the concentration of cGMP suggests that opening of the pore is triggered by cooperative binding of at least three cGMP molecules.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/321070a0</identifier><identifier>PMID: 2422559</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biological and medical sciences ; Cations ; Cell physiology ; Conductance ; cyclic GMP ; Cyclic GMP - pharmacology ; Electric Conductivity ; Eyes & eyesight ; Fundamental and applied biological sciences. Psychology ; Humanities and Social Sciences ; In Vitro Techniques ; Ion Channels - drug effects ; Ion Channels - physiology ; Ion transport ; letter ; membrane currents ; Membrane Potentials - drug effects ; Molecular and cellular biology ; multidisciplinary ; Necturus ; Photoreceptor Cells - physiology ; Pores ; retina ; Rod Cell Outer Segment - drug effects ; Rod Cell Outer Segment - physiology ; rod outer segment membranes ; Science ; Science (multidisciplinary) ; Urodela ; Vision, photoreception</subject><ispartof>Nature (London), 1986-05, Vol.321 (6065), p.70-72</ispartof><rights>Springer Nature Limited 1986</rights><rights>1987 INIST-CNRS</rights><rights>Copyright Macmillan Journals Ltd. May 1, 1986</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-3ffbb5a8cdf2111a6a20b54fe8fe9d8ac6d0e4ca0dd6f209b5be7ec71991bc723</citedby><cites>FETCH-LOGICAL-c453t-3ffbb5a8cdf2111a6a20b54fe8fe9d8ac6d0e4ca0dd6f209b5be7ec71991bc723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/321070a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/321070a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,2727,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7909301$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2422559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zimmerman, A. L</creatorcontrib><creatorcontrib>Baylor, D. A</creatorcontrib><title>Cyclic GMP-sensitive conductance of retinal rods consists of aqueous pores</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>The surface membrane of retinal rod and cone outer segments contains a cation-selective conductance which is activated by 3′,5′-cyclic guanosine monophosphate (cGMP)
1–4
. Reduction of this conductance by a light-induced decrease in the cytoplasmic concentration of cGMP appears to generate the electrical response to light (reviewed in refs 5, 6), but little is known about the molecular nature of the conductance. The estimated unitary conductance is so small
1,7–9
that ion transport might occur via either a carrier or a pore mechanism. Here we report recordings of cGMP-activated single-channel currents from excised rod outer segment patches bathed in solutions low in divalent cations. Two elementary conductances, of ∼24 and 8 pS, were observed. These conductances are too large to be accounted for by carrier transport, indicating that the cGMP-activated conductance consists of aqueous pores. The dependence of the channel activation on the concentration of cGMP suggests that opening of the pore is triggered by cooperative binding of at least three cGMP molecules.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cations</subject><subject>Cell physiology</subject><subject>Conductance</subject><subject>cyclic GMP</subject><subject>Cyclic GMP - pharmacology</subject><subject>Electric Conductivity</subject><subject>Eyes & eyesight</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humanities and Social Sciences</subject><subject>In Vitro Techniques</subject><subject>Ion Channels - drug effects</subject><subject>Ion Channels - physiology</subject><subject>Ion transport</subject><subject>letter</subject><subject>membrane currents</subject><subject>Membrane Potentials - drug effects</subject><subject>Molecular and cellular biology</subject><subject>multidisciplinary</subject><subject>Necturus</subject><subject>Photoreceptor Cells - physiology</subject><subject>Pores</subject><subject>retina</subject><subject>Rod Cell Outer Segment - drug effects</subject><subject>Rod Cell Outer Segment - physiology</subject><subject>rod outer segment membranes</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Urodela</subject><subject>Vision, photoreception</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1r3DAQBmARGtLNB_QPtJhQSnpwMvqy5GNY0jQhJTm0ZyPLo-Lgtbcau7D_vjK72UIp5KTD-zAz6GXsHYdLDtJeScHBgIMDtuDKFLkqrHnDFgDC5mBl8ZYdEz0DgOZGHbEjoYTQulyw--XGd63Pbr895YQ9tWP7GzM_9M3kR9d7zIaQRRzb3nVZHBqaM2pppDlwvyYcJsrWQ0Q6ZYfBdYRnu_eE_fhy8335NX94vL1bXj_kXmk55jKEutbO-iYIzrkrnIBaq4A2YNlY54sGUHkHTVMEAWWtazToDS9LXnsj5An7tJ27jkPaT2O1aslj17l-PqYyheVWcPkqlDohI_mrkCtppTaQ4Pk_8HmYYvoaqgQoBenXVUIXW-TjQBQxVOvYrlzcVByqua3qpa1E3-_mTfUKmz3c1ZPyj7vckXddiKmRlvbMlFBKmO__vGWUkv4nxr9n_Wflh63t3ThF3M_agz9YxrI4</recordid><startdate>19860501</startdate><enddate>19860501</enddate><creator>Zimmerman, A. 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L</au><au>Baylor, D. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cyclic GMP-sensitive conductance of retinal rods consists of aqueous pores</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1986-05-01</date><risdate>1986</risdate><volume>321</volume><issue>6065</issue><spage>70</spage><epage>72</epage><pages>70-72</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>The surface membrane of retinal rod and cone outer segments contains a cation-selective conductance which is activated by 3′,5′-cyclic guanosine monophosphate (cGMP)
1–4
. Reduction of this conductance by a light-induced decrease in the cytoplasmic concentration of cGMP appears to generate the electrical response to light (reviewed in refs 5, 6), but little is known about the molecular nature of the conductance. The estimated unitary conductance is so small
1,7–9
that ion transport might occur via either a carrier or a pore mechanism. Here we report recordings of cGMP-activated single-channel currents from excised rod outer segment patches bathed in solutions low in divalent cations. Two elementary conductances, of ∼24 and 8 pS, were observed. These conductances are too large to be accounted for by carrier transport, indicating that the cGMP-activated conductance consists of aqueous pores. The dependence of the channel activation on the concentration of cGMP suggests that opening of the pore is triggered by cooperative binding of at least three cGMP molecules.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>2422559</pmid><doi>10.1038/321070a0</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1986-05, Vol.321 (6065), p.70-72 |
| issn | 0028-0836 1476-4687 |
| language | eng |
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| source | MEDLINE; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological and medical sciences Cations Cell physiology Conductance cyclic GMP Cyclic GMP - pharmacology Electric Conductivity Eyes & eyesight Fundamental and applied biological sciences. Psychology Humanities and Social Sciences In Vitro Techniques Ion Channels - drug effects Ion Channels - physiology Ion transport letter membrane currents Membrane Potentials - drug effects Molecular and cellular biology multidisciplinary Necturus Photoreceptor Cells - physiology Pores retina Rod Cell Outer Segment - drug effects Rod Cell Outer Segment - physiology rod outer segment membranes Science Science (multidisciplinary) Urodela Vision, photoreception |
| title | Cyclic GMP-sensitive conductance of retinal rods consists of aqueous pores |
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