The fibronectin isoform containing the ed‐b oncofetal domain: A marker of angiogenesis
Different fibronectin (FN) isoforms are generated by the alternative splicing of 3 regions (ED‐A, ED‐B and IIICS) of the primary transcript. The FN isoform containing the ED‐B sequence, a complete type‐III‐homology repeat, while having extremely restricted distribution in normal adult tissues, revea...
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| Veröffentlicht in: | International journal of cancer 1994-12, Vol.59 (5), p.612-618 |
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| container_title | International journal of cancer |
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| creator | Castellani, Patrizia Viale, Giuseppe Dorcaratto, Alessandra Nicolo, Guido Kaczmarek, Janusz Querze, Germano Zardi, Luciano |
| description | Different fibronectin (FN) isoforms are generated by the alternative splicing of 3 regions (ED‐A, ED‐B and IIICS) of the primary transcript. The FN isoform containing the ED‐B sequence, a complete type‐III‐homology repeat, while having extremely restricted distribution in normal adult tissues, reveals high expression in fetal and tumor tissues. Using the monoclonal antibody (MAb) BC‐1, specific for the FN isoform containing the ED‐B sequence (B+·FN), we demonstrate here, using immunohistochemical techniques, that while this FN isoform is undetectable in mature vessels, it is highly expressed during angiogenesis both in neoplastic and in normal tissues, as in the case of the functional layer of endometrium during the proliferative phase. B+·FN is thus a marker for the formation of new vessels, and the BC‐I MAb may be a useful reagent for evaluating the level of the angiogenetic process in different neoplasms. |
| doi_str_mv | 10.1002/ijc.2910590507 |
| format | Article |
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The FN isoform containing the ED‐B sequence, a complete type‐III‐homology repeat, while having extremely restricted distribution in normal adult tissues, reveals high expression in fetal and tumor tissues. Using the monoclonal antibody (MAb) BC‐1, specific for the FN isoform containing the ED‐B sequence (B+·FN), we demonstrate here, using immunohistochemical techniques, that while this FN isoform is undetectable in mature vessels, it is highly expressed during angiogenesis both in neoplastic and in normal tissues, as in the case of the functional layer of endometrium during the proliferative phase. B+·FN is thus a marker for the formation of new vessels, and the BC‐I MAb may be a useful reagent for evaluating the level of the angiogenetic process in different neoplasms.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910590507</identifier><identifier>PMID: 7525495</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Alternative Splicing ; Antibodies, Monoclonal ; Astrocytoma - blood supply ; Biological and medical sciences ; Endometrium - blood supply ; Endometrium - chemistry ; Endothelium, Vascular - pathology ; Factor VIII - immunology ; Female ; Fetus - metabolism ; Fibronectins - analysis ; Fibronectins - genetics ; Glioblastoma - blood supply ; Host-tumor relations. Immunology. Biological markers ; Humans ; Hyperplasia ; Immunoenzyme Techniques ; Medical sciences ; Meningioma - blood supply ; Neoplasms - blood supply ; Neoplasms - chemistry ; Neoplasms - pathology ; Neovascularization, Pathologic ; Tissue Distribution ; Tumors</subject><ispartof>International journal of cancer, 1994-12, Vol.59 (5), p.612-618</ispartof><rights>Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4007-5fd12bb41c8d6e856848cd48cce1aaeae03f6798043e5e98bc6081392ae3f9a73</citedby><cites>FETCH-LOGICAL-c4007-5fd12bb41c8d6e856848cd48cce1aaeae03f6798043e5e98bc6081392ae3f9a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.2910590507$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.2910590507$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3358275$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7525495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castellani, Patrizia</creatorcontrib><creatorcontrib>Viale, Giuseppe</creatorcontrib><creatorcontrib>Dorcaratto, Alessandra</creatorcontrib><creatorcontrib>Nicolo, Guido</creatorcontrib><creatorcontrib>Kaczmarek, Janusz</creatorcontrib><creatorcontrib>Querze, Germano</creatorcontrib><creatorcontrib>Zardi, Luciano</creatorcontrib><title>The fibronectin isoform containing the ed‐b oncofetal domain: A marker of angiogenesis</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>Different fibronectin (FN) isoforms are generated by the alternative splicing of 3 regions (ED‐A, ED‐B and IIICS) of the primary transcript. The FN isoform containing the ED‐B sequence, a complete type‐III‐homology repeat, while having extremely restricted distribution in normal adult tissues, reveals high expression in fetal and tumor tissues. Using the monoclonal antibody (MAb) BC‐1, specific for the FN isoform containing the ED‐B sequence (B+·FN), we demonstrate here, using immunohistochemical techniques, that while this FN isoform is undetectable in mature vessels, it is highly expressed during angiogenesis both in neoplastic and in normal tissues, as in the case of the functional layer of endometrium during the proliferative phase. B+·FN is thus a marker for the formation of new vessels, and the BC‐I MAb may be a useful reagent for evaluating the level of the angiogenetic process in different neoplasms.</description><subject>Alternative Splicing</subject><subject>Antibodies, Monoclonal</subject><subject>Astrocytoma - blood supply</subject><subject>Biological and medical sciences</subject><subject>Endometrium - blood supply</subject><subject>Endometrium - chemistry</subject><subject>Endothelium, Vascular - pathology</subject><subject>Factor VIII - immunology</subject><subject>Female</subject><subject>Fetus - metabolism</subject><subject>Fibronectins - analysis</subject><subject>Fibronectins - genetics</subject><subject>Glioblastoma - blood supply</subject><subject>Host-tumor relations. Immunology. Biological markers</subject><subject>Humans</subject><subject>Hyperplasia</subject><subject>Immunoenzyme Techniques</subject><subject>Medical sciences</subject><subject>Meningioma - blood supply</subject><subject>Neoplasms - blood supply</subject><subject>Neoplasms - chemistry</subject><subject>Neoplasms - pathology</subject><subject>Neovascularization, Pathologic</subject><subject>Tissue Distribution</subject><subject>Tumors</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLFOHDEQhi0UBMeFlg7JRUS3x3h3vbbToRMJREg0h5Ru5fWOD8OuTew9Ibo8Qp4xTxKjO0E6CmuK_5vxzEfICYMFAyjP3YNZlIoBV8BB7JEZAyUKKBn_RGYZgEKwqjkkRyk9ADDGoT4gB4KXvFZ8Rn6u7pFa18Xg0UzOU5eCDXGkJvhJO-_8mk4Zwf7v7z8dDd4Ei5MeaB_GHH-lF3TU8REjDZZqv3ZhjR6TS5_JvtVDwuNdnZO7b5er5VVxc_v9enlxU5gaQBTc9qzsupoZ2TcoeSNrafr8DDKtUSNUthFKQl0hRyU704BklSo1VlZpUc3J2XbuUwy_NpimdnTJ4DBoj2GTWtFIxvOtH4Isg9kPZHCxBU0MKUW07VN0-ciXlkH76rzNztt357nhdDd5043Yv-E7yTn_sst1MnqwUXvj0htWVVyW4hVTW-zZDfjywaft9Y_lfyv8A_AwmuE</recordid><startdate>19941201</startdate><enddate>19941201</enddate><creator>Castellani, Patrizia</creator><creator>Viale, Giuseppe</creator><creator>Dorcaratto, Alessandra</creator><creator>Nicolo, Guido</creator><creator>Kaczmarek, Janusz</creator><creator>Querze, Germano</creator><creator>Zardi, Luciano</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19941201</creationdate><title>The fibronectin isoform containing the ed‐b oncofetal domain: A marker of angiogenesis</title><author>Castellani, Patrizia ; Viale, Giuseppe ; Dorcaratto, Alessandra ; Nicolo, Guido ; Kaczmarek, Janusz ; Querze, Germano ; Zardi, Luciano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4007-5fd12bb41c8d6e856848cd48cce1aaeae03f6798043e5e98bc6081392ae3f9a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alternative Splicing</topic><topic>Antibodies, Monoclonal</topic><topic>Astrocytoma - blood supply</topic><topic>Biological and medical sciences</topic><topic>Endometrium - blood supply</topic><topic>Endometrium - chemistry</topic><topic>Endothelium, Vascular - pathology</topic><topic>Factor VIII - immunology</topic><topic>Female</topic><topic>Fetus - metabolism</topic><topic>Fibronectins - analysis</topic><topic>Fibronectins - genetics</topic><topic>Glioblastoma - blood supply</topic><topic>Host-tumor relations. Immunology. Biological markers</topic><topic>Humans</topic><topic>Hyperplasia</topic><topic>Immunoenzyme Techniques</topic><topic>Medical sciences</topic><topic>Meningioma - blood supply</topic><topic>Neoplasms - blood supply</topic><topic>Neoplasms - chemistry</topic><topic>Neoplasms - pathology</topic><topic>Neovascularization, Pathologic</topic><topic>Tissue Distribution</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castellani, Patrizia</creatorcontrib><creatorcontrib>Viale, Giuseppe</creatorcontrib><creatorcontrib>Dorcaratto, Alessandra</creatorcontrib><creatorcontrib>Nicolo, Guido</creatorcontrib><creatorcontrib>Kaczmarek, Janusz</creatorcontrib><creatorcontrib>Querze, Germano</creatorcontrib><creatorcontrib>Zardi, Luciano</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castellani, Patrizia</au><au>Viale, Giuseppe</au><au>Dorcaratto, Alessandra</au><au>Nicolo, Guido</au><au>Kaczmarek, Janusz</au><au>Querze, Germano</au><au>Zardi, Luciano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The fibronectin isoform containing the ed‐b oncofetal domain: A marker of angiogenesis</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1994-12-01</date><risdate>1994</risdate><volume>59</volume><issue>5</issue><spage>612</spage><epage>618</epage><pages>612-618</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>Different fibronectin (FN) isoforms are generated by the alternative splicing of 3 regions (ED‐A, ED‐B and IIICS) of the primary transcript. The FN isoform containing the ED‐B sequence, a complete type‐III‐homology repeat, while having extremely restricted distribution in normal adult tissues, reveals high expression in fetal and tumor tissues. Using the monoclonal antibody (MAb) BC‐1, specific for the FN isoform containing the ED‐B sequence (B+·FN), we demonstrate here, using immunohistochemical techniques, that while this FN isoform is undetectable in mature vessels, it is highly expressed during angiogenesis both in neoplastic and in normal tissues, as in the case of the functional layer of endometrium during the proliferative phase. B+·FN is thus a marker for the formation of new vessels, and the BC‐I MAb may be a useful reagent for evaluating the level of the angiogenetic process in different neoplasms.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7525495</pmid><doi>10.1002/ijc.2910590507</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0020-7136 |
| ispartof | International journal of cancer, 1994-12, Vol.59 (5), p.612-618 |
| issn | 0020-7136 1097-0215 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Alternative Splicing Antibodies, Monoclonal Astrocytoma - blood supply Biological and medical sciences Endometrium - blood supply Endometrium - chemistry Endothelium, Vascular - pathology Factor VIII - immunology Female Fetus - metabolism Fibronectins - analysis Fibronectins - genetics Glioblastoma - blood supply Host-tumor relations. Immunology. Biological markers Humans Hyperplasia Immunoenzyme Techniques Medical sciences Meningioma - blood supply Neoplasms - blood supply Neoplasms - chemistry Neoplasms - pathology Neovascularization, Pathologic Tissue Distribution Tumors |
| title | The fibronectin isoform containing the ed‐b oncofetal domain: A marker of angiogenesis |
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