A complex that contains proteins binding to the PSE and TATA sites in a human U6 small nuclear RNA promoter
The proximal promoter of a human U6 small nuclear RNA (snRNA)-encoding gene contains two separate elements, the proximal sequence element ( PSE) and the TATA box. We investigated the interaction of the PSE- and TATA-binding proteins (PBP and TBP) with normal and mutant U6 proximal promoters using an...
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| Veröffentlicht in: | Gene 1994-10, Vol.148 (2), p.269-275 |
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| container_title | Gene |
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| creator | Goomer, Randal S. Urso, Olgui Kunkel, Gary R. |
| description | The proximal promoter of a human
U6 small nuclear RNA (snRNA)-encoding gene contains two separate elements, the proximal sequence element (
PSE) and the TATA box. We investigated the interaction of the
PSE- and TATA-binding proteins (PBP and TBP) with normal and mutant
U6 proximal promoters using an electrophoretic mobility shift assay. We detected a complex containing both PBP and TBP bound to the wild-type
U6 promoter. Efficient formation of the triple complex was dependent on the presence of the
PSE and the TATA box on the template DNA. Mutant U6 promoters containing an increased spacing between the PSE and TATA box of 5 or 10 bp were impaired in the ability to form a complex that includes TBP. We infer from these results that PBP and TBP interact when their binding sites are properly positioned in a
U6 gene promoter. Eukaryotic promoters; RNA polymerase III; snRNA gene transcription factors |
| doi_str_mv | 10.1016/0378-1119(94)90698-X |
| format | Article |
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U6 small nuclear RNA (snRNA)-encoding gene contains two separate elements, the proximal sequence element (
PSE) and the TATA box. We investigated the interaction of the
PSE- and TATA-binding proteins (PBP and TBP) with normal and mutant
U6 proximal promoters using an electrophoretic mobility shift assay. We detected a complex containing both PBP and TBP bound to the wild-type
U6 promoter. Efficient formation of the triple complex was dependent on the presence of the
PSE and the TATA box on the template DNA. Mutant U6 promoters containing an increased spacing between the PSE and TATA box of 5 or 10 bp were impaired in the ability to form a complex that includes TBP. We infer from these results that PBP and TBP interact when their binding sites are properly positioned in a
U6 gene promoter. Eukaryotic promoters; RNA polymerase III; snRNA gene transcription factors</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(94)90698-X</identifier><identifier>PMID: 7958954</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Base Sequence ; DNA Primers ; DNA-Binding Proteins - metabolism ; Humans ; Molecular Sequence Data ; Mutation ; Promoter Regions, Genetic ; RNA, Small Nuclear - genetics ; TATA Box ; TATA-Box Binding Protein ; Transcription Factors - metabolism ; Transcription, Genetic</subject><ispartof>Gene, 1994-10, Vol.148 (2), p.269-275</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-f4aa346628f383dec0272892c22bcd55ee4d155938f35d33e13daf30ae5a46213</citedby><cites>FETCH-LOGICAL-c388t-f4aa346628f383dec0272892c22bcd55ee4d155938f35d33e13daf30ae5a46213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(94)90698-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7958954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goomer, Randal S.</creatorcontrib><creatorcontrib>Urso, Olgui</creatorcontrib><creatorcontrib>Kunkel, Gary R.</creatorcontrib><title>A complex that contains proteins binding to the PSE and TATA sites in a human U6 small nuclear RNA promoter</title><title>Gene</title><addtitle>Gene</addtitle><description>The proximal promoter of a human
U6 small nuclear RNA (snRNA)-encoding gene contains two separate elements, the proximal sequence element (
PSE) and the TATA box. We investigated the interaction of the
PSE- and TATA-binding proteins (PBP and TBP) with normal and mutant
U6 proximal promoters using an electrophoretic mobility shift assay. We detected a complex containing both PBP and TBP bound to the wild-type
U6 promoter. Efficient formation of the triple complex was dependent on the presence of the
PSE and the TATA box on the template DNA. Mutant U6 promoters containing an increased spacing between the PSE and TATA box of 5 or 10 bp were impaired in the ability to form a complex that includes TBP. We infer from these results that PBP and TBP interact when their binding sites are properly positioned in a
U6 gene promoter. Eukaryotic promoters; RNA polymerase III; snRNA gene transcription factors</description><subject>Base Sequence</subject><subject>DNA Primers</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Small Nuclear - genetics</subject><subject>TATA Box</subject><subject>TATA-Box Binding Protein</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKkvhG4DkE4JDwP9jX5CiqrRIFSDYSr1ZXntCDYmztRME3x6HXfUIc_FY780b6TcIPafkDSVUvSW81Q2l1Lwy4rUhyujm5gHaUN2ahhCuH6LNveUxelLKd1JLSnaCTlojtZFig3502E_jfoBfeL51c_2k2cVU8D5PM6zNLqYQ0zc8T9UB-PPXc-xSwNtu2-ESZyg4Juzw7TK6hK8VLqMbBpwWP4DL-MvHbo0aa1h-ih71bijw7Pieouv359uzy-bq08WHs-6q8VzruemFc1woxXTPNQ_gCWuZNswztvNBSgARqJSGV10GzoHy4HpOHEgnFKP8FL085NbFdwuU2Y6xeBgGl2Baim2VpkxURP8zUqW0UC2rRnEw-jyVkqG3-xxHl39bSux6DLuStitpa4T9ewx7U8deHPOX3QjhfuhIv-rvDjpUGj8jZFt8hOQhxAx-tmGK_17wB08Ol8M</recordid><startdate>19941021</startdate><enddate>19941021</enddate><creator>Goomer, Randal S.</creator><creator>Urso, Olgui</creator><creator>Kunkel, Gary R.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19941021</creationdate><title>A complex that contains proteins binding to the PSE and TATA sites in a human U6 small nuclear RNA promoter</title><author>Goomer, Randal S. ; Urso, Olgui ; Kunkel, Gary R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-f4aa346628f383dec0272892c22bcd55ee4d155938f35d33e13daf30ae5a46213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Base Sequence</topic><topic>DNA Primers</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Small Nuclear - genetics</topic><topic>TATA Box</topic><topic>TATA-Box Binding Protein</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goomer, Randal S.</creatorcontrib><creatorcontrib>Urso, Olgui</creatorcontrib><creatorcontrib>Kunkel, Gary R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goomer, Randal S.</au><au>Urso, Olgui</au><au>Kunkel, Gary R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A complex that contains proteins binding to the PSE and TATA sites in a human U6 small nuclear RNA promoter</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1994-10-21</date><risdate>1994</risdate><volume>148</volume><issue>2</issue><spage>269</spage><epage>275</epage><pages>269-275</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The proximal promoter of a human
U6 small nuclear RNA (snRNA)-encoding gene contains two separate elements, the proximal sequence element (
PSE) and the TATA box. We investigated the interaction of the
PSE- and TATA-binding proteins (PBP and TBP) with normal and mutant
U6 proximal promoters using an electrophoretic mobility shift assay. We detected a complex containing both PBP and TBP bound to the wild-type
U6 promoter. Efficient formation of the triple complex was dependent on the presence of the
PSE and the TATA box on the template DNA. Mutant U6 promoters containing an increased spacing between the PSE and TATA box of 5 or 10 bp were impaired in the ability to form a complex that includes TBP. We infer from these results that PBP and TBP interact when their binding sites are properly positioned in a
U6 gene promoter. Eukaryotic promoters; RNA polymerase III; snRNA gene transcription factors</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7958954</pmid><doi>10.1016/0378-1119(94)90698-X</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1994-10, Vol.148 (2), p.269-275 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76812400 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Base Sequence DNA Primers DNA-Binding Proteins - metabolism Humans Molecular Sequence Data Mutation Promoter Regions, Genetic RNA, Small Nuclear - genetics TATA Box TATA-Box Binding Protein Transcription Factors - metabolism Transcription, Genetic |
| title | A complex that contains proteins binding to the PSE and TATA sites in a human U6 small nuclear RNA promoter |
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