Use of HPLC to measure circulating adenosine levels in migrainous patients
Adenosine is a powerful natural vasodilator that could be involved in migraine. It is difficult to assay this nucleoside, however, because it has a short half-life. We have used HPLC to compare the concentrations of blood adenosine sampled in crisis-free intervals and during crisis periods in ten pa...
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| Veröffentlicht in: | Clinica chimica acta 1994-06, Vol.227 (1), p.185-194 |
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| container_title | Clinica chimica acta |
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| creator | Guieu, R. Sampiéri, F. Bechis, G. Rochat, H. |
| description | Adenosine is a powerful natural vasodilator that could be involved in migraine. It is difficult to assay this nucleoside, however, because it has a short half-life. We have used HPLC to compare the concentrations of blood adenosine sampled in crisis-free intervals and during crisis periods in ten patients with common migraine and have compared these levels to those noted in a control population. Our sampling technique uses vacuum suction and enables rapid mixing of the blocking solution and whole venous blood. This results in reproducible HPLC assays. We also show that, during a migraine crisis, mean blood adenosine levels increase by 47%. However, the origin of this adenosine release is difficult to define. |
| doi_str_mv | 10.1016/0009-8981(94)90146-5 |
| format | Article |
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It is difficult to assay this nucleoside, however, because it has a short half-life. We have used HPLC to compare the concentrations of blood adenosine sampled in crisis-free intervals and during crisis periods in ten patients with common migraine and have compared these levels to those noted in a control population. Our sampling technique uses vacuum suction and enables rapid mixing of the blocking solution and whole venous blood. This results in reproducible HPLC assays. We also show that, during a migraine crisis, mean blood adenosine levels increase by 47%. 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| ispartof | Clinica chimica acta, 1994-06, Vol.227 (1), p.185-194 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Adenosine - blood Adult Biological and medical sciences Chromatography, High Pressure Liquid - methods Female HPLC Humans Male Medical sciences Middle Aged Migraine Migraine Disorders - blood Neurology Vascular diseases and vascular malformations of the nervous system |
| title | Use of HPLC to measure circulating adenosine levels in migrainous patients |
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