The unlabeled antibody method: comparison of peroxidase-antiperoxidase with avidin-biotin complex by a new method of quantification

Upon plotting of areas against optical densities in immunocytochemically stained tissue sections, hyperbolic curves were obtained which could be reduced to two straight lines, one representing variations in stained structures, and the other variations in background. The slopes of the stained structu...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 1986-05, Vol.34 (5), p.599-605
Hauptverfasser:	Sternberger, LA, Sternberger, NH
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Avidin Biological and medical sciences Biotin Brain - cytology Diverse techniques Fundamental and applied biological sciences. Psychology Histocytochemistry - methods Horseradish Peroxidase - metabolism Immunoenzyme Techniques Male Molecular and cellular biology Rats Rats, Inbred Strains
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