Adaptation of a commercially available indirect fluorescent antibody slide test for measuring measles-specific immunoglobulins
A commercially available indirect fluorescent antibody (IFA) test was evaluated for the determination of measles-specific immunoglobulins G and M (MIgG, MIgM). The IFA test detected fourfold rises of MIgG in 34 of 35 (97%) cases of measles confirmed by complement fixation or hemagglutination inhibit...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 1986-04, Vol.4 (4), p.285-290 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Kleiman, Martin B. Bockhold, Carol A. Zimmerman, Sarah E. Griffin, Charles French, Morris L.V. Barrett, Charles |
| description | A commercially available indirect fluorescent antibody (IFA) test was evaluated for the determination of measles-specific immunoglobulins G and M (MIgG, MIgM). The IFA test detected fourfold rises of MIgG in 34 of 35 (97%) cases of measles confirmed by complement fixation or hemagglutination inhibition. In determining immune status, MIgG-IFA correlated with hemagglutination inhibition in 22 of 23 (96%) cases. The IFA test detected MIgM in only 11 of 34 acute-phase sera collected within 5 days of the reported onset of rash and in the convalescent specimens of another 13 of the 35 specimens. The IFA test is an effective method for the conventional diagnosis of measles and for determining immune status. This IFA test has a limited role as a rapid diagnostic test for measles when used to detect measles-specific MIgM in acute-phase sera obtained from patients with suspected measles. |
| doi_str_mv | 10.1016/0732-8893(86)90068-4 |
| format | Article |
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The IFA test detected fourfold rises of MIgG in 34 of 35 (97%) cases of measles confirmed by complement fixation or hemagglutination inhibition. In determining immune status, MIgG-IFA correlated with hemagglutination inhibition in 22 of 23 (96%) cases. The IFA test detected MIgM in only 11 of 34 acute-phase sera collected within 5 days of the reported onset of rash and in the convalescent specimens of another 13 of the 35 specimens. The IFA test is an effective method for the conventional diagnosis of measles and for determining immune status. 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The IFA test detected fourfold rises of MIgG in 34 of 35 (97%) cases of measles confirmed by complement fixation or hemagglutination inhibition. In determining immune status, MIgG-IFA correlated with hemagglutination inhibition in 22 of 23 (96%) cases. The IFA test detected MIgM in only 11 of 34 acute-phase sera collected within 5 days of the reported onset of rash and in the convalescent specimens of another 13 of the 35 specimens. The IFA test is an effective method for the conventional diagnosis of measles and for determining immune status. 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Bockhold, Carol A. ; Zimmerman, Sarah E. ; Griffin, Charles ; French, Morris L.V. ; Barrett, Charles</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-d57401be6335acd0175431c199a04d5bc25c4ab382e47550720590bccd10c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Agglutination Tests</topic><topic>Antibodies, Viral - analysis</topic><topic>Antigens, Viral</topic><topic>Biological and medical sciences</topic><topic>Complement Fixation Tests</topic><topic>Fluorescent Antibody Technique</topic><topic>Hemagglutination Inhibition Tests</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin M - analysis</topic><topic>Immunoglobulins - analysis</topic><topic>Infectious diseases</topic><topic>Measles - immunology</topic><topic>Measles virus - immunology</topic><topic>Medical sciences</topic><topic>Viral diseases</topic><topic>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kleiman, Martin B.</creatorcontrib><creatorcontrib>Bockhold, Carol A.</creatorcontrib><creatorcontrib>Zimmerman, Sarah E.</creatorcontrib><creatorcontrib>Griffin, Charles</creatorcontrib><creatorcontrib>French, Morris L.V.</creatorcontrib><creatorcontrib>Barrett, Charles</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kleiman, Martin B.</au><au>Bockhold, Carol A.</au><au>Zimmerman, Sarah E.</au><au>Griffin, Charles</au><au>French, Morris L.V.</au><au>Barrett, Charles</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adaptation of a commercially available indirect fluorescent antibody slide test for measuring measles-specific immunoglobulins</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>1986-04-01</date><risdate>1986</risdate><volume>4</volume><issue>4</issue><spage>285</spage><epage>290</epage><pages>285-290</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>A commercially available indirect fluorescent antibody (IFA) test was evaluated for the determination of measles-specific immunoglobulins G and M (MIgG, MIgM). The IFA test detected fourfold rises of MIgG in 34 of 35 (97%) cases of measles confirmed by complement fixation or hemagglutination inhibition. In determining immune status, MIgG-IFA correlated with hemagglutination inhibition in 22 of 23 (96%) cases. The IFA test detected MIgM in only 11 of 34 acute-phase sera collected within 5 days of the reported onset of rash and in the convalescent specimens of another 13 of the 35 specimens. The IFA test is an effective method for the conventional diagnosis of measles and for determining immune status. This IFA test has a limited role as a rapid diagnostic test for measles when used to detect measles-specific MIgM in acute-phase sera obtained from patients with suspected measles.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>3516550</pmid><doi>10.1016/0732-8893(86)90068-4</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0732-8893 |
| ispartof | Diagnostic microbiology and infectious disease, 1986-04, Vol.4 (4), p.285-290 |
| issn | 0732-8893 1879-0070 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Agglutination Tests Antibodies, Viral - analysis Antigens, Viral Biological and medical sciences Complement Fixation Tests Fluorescent Antibody Technique Hemagglutination Inhibition Tests Human viral diseases Humans Immunoglobulin G - analysis Immunoglobulin M - analysis Immunoglobulins - analysis Infectious diseases Measles - immunology Measles virus - immunology Medical sciences Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye |
| title | Adaptation of a commercially available indirect fluorescent antibody slide test for measuring measles-specific immunoglobulins |
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